... selection marker such as thymidine kinase, a researcher may subsequently kill off any cells that still...later restores the target sequence. This allows researchers to avoid making unnecessary base pair changes...powerful technology that may be used for a variety of research applications, including making transgenic cell...molecular and cellular biology and brings 10 years of research experience with stem cells to Transposagen's cell...Mathew applies his specialized knowledge to help researchers identify the right technologies and strategies...

...on the right-hand side of the search page to narrow your keyword search to one of these organisms. Help...plasmid kits and other resources for microbial research. Kits, guides, plasmids, and more We have Plasmid...resources that we created filters just for them on our search page. Use the Expression and Insert Species filters...

...continuation of our efforts to provide resources to researchers in developing countries or that work in under-funded...continued opportunity to get plasmids to the researchers who need them the most. Check back here to learn...more about future awardees and their inspiring research. Additional Resources on the Addgene Blog Learn...Resources Learn About Our Grants to Rare Disease Research Resources on Addgene.org Check out our Cancer...

...you’re new to the topic. Or, try the search bar Now, you can search for the articles you want to find without...on the search bar at the top of the screen and type in what you’re looking for. After you search, you’ll...

... us to calculate how much time we have saved researchers. In a recent blog post, we looked at how long... plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time to make each...are always on the lookout for new ways to help researchers facilitate and accelerate scientific discovery...discovery. How can Addgene help accelerate your research? Let us know! ...

... Mentor Learn How the Future of Research Looks to Improve the Research Process  Browse our Career Posts...Plasmids Find Plasmids for Your Research ... research project...

...important. Antibodies are a critical component of the researcher toolkit due to their ability to bind to molecular...culprits in the reproducibility crisis of biomedical research (Baker, 2015; Bradbury & Plükthorn, 2015).  To...which represent five experimental strategies researchers can use to show that their antibody performs ...Reproducibility: Standardize antibodies used in research. Nature 518:27–9. https://doi.org/10.1038/518027a...finding and validating suitable antibodies for research. Mabs 8:27–36. https://doi.org/10.1080/19420862.2015.1100787...

...progress their research. *2015 total reflects the number of distributed plasmids as of March 26, 2015. How...information in our lab software, labeling stabs with barcodes as they go. Stabs are organized by shipment and...and moved to our packing area.Using the barcode system, the correct plasmids are packed into their boxes...

... and cloned by Neville Sanjana. This fantastic research tool has been requested from the repository nearly...over a year and a half, a small sampling of the research it has already been used for includes work probing...plasmids being distributed to and accelerating research in labs all around the world. We thank our many...depositors for making these reagents available to the research community through Addgene and look forward to ...

...fluorescent proteins like GFP came to be used as research tools is particularly interesting. Although humans...light and glows green. In the late 1980s, another researcher, Douglas Prasher, got the idea that this new ...of GFP, and began to realize its potential as a research tool. Soon, the number of people studying it grew...proteins with new colors Around the same time, researcher Sergey Lukyanov realized that, although they ...

...Permission to use materials for research or teaching purposes Rights to all research results, modifications,...understand - you’re a busy scientist working hard on research, and you need the right experimental materials... receiving institutions and their respective researchers. Check out the lists below for more details. ...

... novel aminoacyl tRNA synthetase/tRNA pair, a researcher can lure the ribosome into inserting a fluorophore-tagged...beyond the FRET range of the donor (i.e. >100 Å). Researchers have developed an extensive array of chemical...currently an Assistant Professor in the Anesthesia Research department at Brigham and Women’s Hospital in ...378-383. PubMed PMID: 12605205. 13. Yan, Qi, and Marcel P. Bruchez. "Advances in chemical labeling of proteins... proteins in living cells." Cell and tissue research 360.1 (2015): 179-194. PubMed PMID: 25743694. PubMed...

...through the use of invasive methods, limiting researchers to accessible, spatially constrained regions.... it’s always helpful to start with a literature search to see what others have done, you’ll need to experimentally...precursor. Considering systemic capsids for your research? While systemic capsids mostly function like your...depicting the phylogenetic relationship of common research species. Among these species, Old World Monkeys...to the nonhuman primate nervous system. Current Research in Neurobiology, 4, 100086. https://doi.org/10.1016... central nervous system. Nature Cardiovascular Research, 1(4), Article 4. https://doi.org/10.1038/s44161... Pelkey, K. A., Chittajallu, R., Guo, B., Arias-Garcia, M. A., Allaway, K., Sakopoulos, S., Schneider,...

...Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project Advice... to build and lead a productive and successful research lab. Once goals are reached, how do science labs...day trip each year and as we do a lot of yeast research, a few of those have been trips to breweries who...

...goals of accelerating research and discovery by improving access to useful research materials and information...impact made possible by the sharing of tools for research: discovered and developed by our depositors, used...ways to support a global community where every researcher feels both welcomed and resourced for success...

...collaborative endeavor providing plasmids to researchers in developing countries. We are proud to introduce...this important agricultural plant. Her current research focuses on endophytes, bacteria, and fungi that...applying gene technology to bacteria and plants. Her research involves cloning cry genes from the soil bacterium...groups of scientists working in a wide range of research fields and geographical locations. We are excited...

...for plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...found in bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature... Produce synthetic viruses that can be used in research or for therapeutics Addgene has compiled various... series, please let us know in the comments. Note:Marcy Patrick contributed to the writing of this article...

...changes in DA concentration. To address this, researchers from some labs have developed fluorescent-protein... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...protocols and videos Find biosensor AAVs for your research ...

...opsins) to manipulate cell activity, enables researchers to silence or incite neuronal firing and study...Identifying neurons that trigger spontaneous swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish... distinguish between swimming behaviors, the researchers measured the tail movements of 5 day old fish... viral service Find optogenetics AAVs for your research Find all AAVs from the viral service ...

...conference. It was inspiring to see how microbiology research is improving lives worldwide and the presentation...the booth. We were happy to chat with lots of researchers who weren’t aware of Addgene’s plasmid sharing... plasmid tools on the horizon for microbiology research and we’d love to help more microbiologists share...being the Director of the Great Lakes Bioenergy Research Center and a fellow Rhodobacter enthusiast, Tim...