We narrowed to 525 results for: ATO

...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...

...academic publishing goals and make you a better communicator overall. Don’t Write in a Vacuum Your research...through many iterations read by your PI, your collaborators, others you know in the field, and even people...

...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would...

...group focuses on gene regulation in nematode molting and spermatogenesis. His group also builds tools to ...

...reliably detect single-base differences. The original creators of this technique visualized the ligation product... the Thermostable Ligase Reaction Temperature Calculator can be used to estimate an appropriate incubation...

...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector...

...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...

...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...

... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...contexts. Most of the immune cells we study are migratory, and their exact localization is important for...

...absorbed by matter, they promote electrons within atoms to a higher energy state. This process is called.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...and purification is a snap using thin layer chromatography (Fessenden, 2009). However, these reagents ...

... electrophysiology data too but that’s what collaborators are for). For example, if you study neural pathways...projection-specific neuronal populations using combinatorial viral approaches. Current protocols in neuroscience...

...any applications. DNA quantity is fairly self explanatory and achieved by performing different sized preps...about!) are a significant source of waste in laboratories. To help combat this, some manufacturers have...

... as law, medical/technical writing, clinical, regulatory, product development, business development, consulting...roles in areas including: research, clinical, regulatory, project management, alliance management, business...

...the the ability to generate teratomas is the gold standard instead. Teratoma formation requires injecting...

...essential to build up our current understanding of neuroanatomy and neural connectivity, providing valuable ...conditional transgenic mice, to reveal not only the anatomy of neuronal connections, but to visualize neuron...

...Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video to Share Your Science...

...the Addgene Blog 5 Science Rap Videos and Their Creator: Tom McFadden SciComm with the Experts at Science...

...lab manager, research technician, or Principal Investigator. How-to guides for troubleshooting your experiments...