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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 573 results for: CHI;

Showing: 281 - 300 of 573 results

  1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification by...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can ...

  3. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...(MAGIC). With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ...

  4. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther...

  6. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...approximately the length of your average PhD to achieve!) Each field has its own framework: a particular...images, and links to pay attention to. If you’re watching a video, you now have moving images, noises and...

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased sensitivity...

  9. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ... of its biggest advantages. Specificity can be achieved using specialized promoters, Flp or Cre dependent...expression AAVs have to infect cells, hijack host cell machinery, and synthesize a second strand of DNA before ...

  10. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...the University of Illinois and the University of Michigan - initially cancelled their plans, but have since...plans and budgets working around issues such as childcare using money from their increased salaries. In ...

  11. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...to your antibody. This chemical coupling can be achieved through several different mechanisms. Most take...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous...

  12. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein...pulldown of a protein of interest, much like using ChIP for studying protein and DNA interactions. Unfortunately...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...Biology at Baylor College of Medicine and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...of Molecular and Cellular Biology, and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...

  14. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...While some of the statements may be slightly far reaching, remember that you are writing a proposal to test...secondary milestones to meet along the way to achieving each aim. A funded proposal typically lists the...

  15. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...inducible promoters, light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas...Mayo-Muñoz D, He F, Jørgensen J, Madsen P, Bhoobalan-Chitty Y, Peng X (2018) Anti-CRISPR-Based and CRISPR-Based...

  18. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...efficiency so that the greatest effect possible will be achieved. On the other hand, if you are trying to knock-in...PAM-flexible genome editing with an engineered chimeric Cas9. Nat Commun 14, 6175 (2023). DOI:10.1038/...

  19. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...musculus (mouse), R. norvegicus (rat), G. gallus (chicken), B. taurus (bovine), X. laevis (frog), D. rerio...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format...

  20. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ... in order to turn Twitter into a paper-finding-machine. There are four general types of accounts you can...Scholar analyzes publications and figures using machine learning to extract important features such as ...
Showing: 281 - 300 of 573 results