We narrowed to 578 results for: CHI;

...tool for labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting... which also provides a useful yet low-pressure teaching opportunity for lab members or students new to...

... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...

...non-interacting with) the cells’ own natural translation machinery; these are often incorporated into the cells via...of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc...

...approaches. However, with each new technical achievement, the complexity of the device increases, as does...with the community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation...

... Those two things, in my opinion, had not been achieved.” That is, until now. Enter the SAM pooled library...demonstrated in a paper in Cell that they could typically achieve 90-99% knockdown with minimal off-target effects...

...(MAGIC). With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ...

... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther...

...DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein...pulldown of a protein of interest, much like using ChIP for studying protein and DNA interactions. Unfortunately...

...including difficult-to-manipulate primary cells, achieving knockdown within 1-2 hr of reagent delivery. TRIM-Away... gene expression regimes that cannot be easily achieved with classical gene overexpression and knockout...

...the University of Illinois and the University of Michigan - initially cancelled their plans, but have since...plans and budgets working around issues such as childcare using money from their increased salaries. In ...

...approximately the length of your average PhD to achieve!) Each field has its own framework: a particular...images, and links to pay attention to. If you’re watching a video, you now have moving images, noises and...

...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased sensitivity...

...to your antibody. This chemical coupling can be achieved through several different mechanisms. Most take...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous...

...design. We all want tools that expand what we can achieve, but are simple to pick up and work well the first...components. Nearly all of us played with LEGO as a child (some of us still do), so we are used to the idea...

... of its biggest advantages. Specificity can be achieved using specialized promoters, Flp or Cre dependent...expression AAVs have to infect cells, hijack host cell machinery, and synthesize a second strand of DNA before ...

... making fluorescent protein fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent...Galvan A, Raper J, Hu X, Paré J, Bonaventura J, Richie CT, Michaelides M, Mueller SAL, Roseboom PH, Oler...

...Biology at Baylor College of Medicine and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...of Molecular and Cellular Biology, and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...

...While some of the statements may be slightly far reaching, remember that you are writing a proposal to test...secondary milestones to meet along the way to achieving each aim. A funded proposal typically lists the...

...expression Generally speaking, one may want to achieve either inhibition or activation of neurons in a...Stoica et al., 2013). Vector delivery may also be achieved via injection into the liquor of the cerebral ...

... expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of...Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui Zeng Additional Resrouces...