Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 659 results for: Clu

Showing: 281 - 300 of 659 results

  2. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...understand and grow the scientific ecosystem, including understanding how shared materials are used to...ll be confident in what to expect. No system, including MTAs, is perfect, but it does provide consistency...

  3. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...libraries. NEB also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated...

  4. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ... browse our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for...best serotype for their particular study, which includes comparing the transduction patterns of different...

  5. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...pivotal role in an array of biological processes including development, the immune system, and cell turnover...been difficult until now (Zhang et al., 2019). Conclusion FlipGFP is a novel fluorogenic reporter that ...

  7. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...are multiple methods of DNA assembly available, including: Gibson Assembly®, BioBrick® Assembly, Golden ...applications of the NEBuilder HiFi DNA Assembly Mix include:  Generation of constructs with multiple point...

  9. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ... new contact to connect on LinkedIn and always include a personal note about where you met and what you...can be as simple as “it was nice meeting you.” Include follow-up questions or requests for advice based...

  10. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...that can influence the HDR/NHEJ decision. Our conclusions reinforce a key scientific principle: that understanding...frequencies of genome cutting and, when donor DNA is included in the nucleofection reaction, extremely high ...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is...in the cell, as many of these experiments are concluded within 48 h of transfection. Of course, it is ...

  13. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...backbone to generate R-mAbs of different subclasses, including subclasses that were not produced by the original...expanded their recombinant affinity reagent tools to include nanobodies. At Addgene, we are excited to be part...

  14. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...Others ran experiments at odd hours, used more secluded workspaces, or politely asked to not be disturbed... of making an error. Try a variety of things, including (and maybe especially) suggestions you are initially...

  15. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...generate antibodies against many types of molecules including proteins, lipids, and carbohydrates, in this blog...2005). This is reflected in the antibody’s cost. Conclusion Whether you choose to use a monoclonal antibody...

  16. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...be activated by an array of different ligands, including hormones, neurotransmitters, photons or odorants... new receptors were termed Designer Receptor Exclusively Activated by Designer Drugs (DREADDs) (Roth, ...

  17. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...we rolled out multiple changes to our website, including the recent homepage and search tool updates. Now...

  18. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...shares many common features with gamma-retrovirus including genome architecture, host integration, and its...its cargo, in fact its viral life cycle does not include genomic integration. While the expression of adenovirus...

  19. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... tools with many applications. These libraries include sgRNAs targeting the entire genome, overexpression...several advantages over traditional vaccines, including 1) cellular responses in addition to antibody ...

  20. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ... is not fluorescence! Chemiluminescence, which includes all bioluminescence, is the production of light...Promega to offer their Promega Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which...
Showing: 281 - 300 of 659 results