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  7. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...you are negotiating. Then take a moment before, during, or after a negotiation to think about the situation...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... inserted. B) Protein expression and labeling procedure. Adapted from Smith et al. 2024 under a CC-BY ...

  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...more gRNAs must be used along the same chromosome. During DNA repair, there is a chance that the two breakpoints...

  10. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ... you can always change your compensation values during the analysis portion of your experiment. So, as...

  11. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...the usefulness of popular CRISPR tools is both enduring and rapidly recognized.   Where is the CRISPR ...

  12. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ... subtypes, thanks to viral serotype specificity during delivery (more on this later). Chemigenetic sensors...

  13. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...their mental model of the protein. At some point during this interrogation process, they will almost invariably...

  15. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...favorite in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than...

  18. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988...

  19. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...various mammalian promoters), the same cloning procedure can be used to incorporate each element into the...

  20. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...al., 2006. Tips: Don’t plate seeds too densely during selection: this will increase background growth...
Showing: 281 - 300 of 356 results