We narrowed to 364 results for: DUR

1. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...plasmid from being cut and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and...

2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

   Type

   Blog Post

   Published

   March 8, 2017, 3:15 p.m.

   ...scientists will contact you if any issues arise during the QC process. As the depositing scientist, you...

3. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...and silences its expression. More specifically, during the process of RNAi, an enzyme dubbed Dicer cuts...

4. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   Published

   Oct. 6, 2020, 1:15 p.m.

   ...regulation. This can be used to activate transcription during either initiation or elongation, depending on which...

5. 6 Tips for Grant Writing

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   Blog Post

   Published

   Oct. 18, 2016, 2:30 p.m.

   ...’s a good idea to move around to different labs during your career; you learn new techniques and interact...

6. Tips for Titering Your Lentiviral Preps

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   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...subcloning, expansion, and confirmation. This procedure can take several months but should be considered...

7. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   Published

   Nov. 19, 2015, 3:30 p.m.

   ... and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period."...

8. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   Blog Post

   Published

   June 5, 2014, 3:32 p.m.

   ...molded the whole thing into a marketable product. During this process, we had an idea that survived the ...

9. Hot Plasmids: Winter 2025

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   Blog Post

   Published

   March 11, 2025, 1:15 p.m.

   ... inserted. B) Protein expression and labeling procedure. Adapted from Smith et al. 2024 under a CC-BY ...

10. Degrading DNA with Cascade-Cas3

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    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...more gRNAs must be used along the same chromosome. During DNA repair, there is a chance that the two breakpoints...

11. Antibodies 101: Flow Compensation

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    Blog Post

    Published

    May 16, 2024, 1:15 p.m.

    ... you can always change your compensation values during the analysis portion of your experiment. So, as...

12. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ...the usefulness of popular CRISPR tools is both enduring and rapidly recognized.   Where is the CRISPR ...

13. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    Published

    Jan. 30, 2024, 2:15 p.m.

    ... subtypes, thanks to viral serotype specificity during delivery (more on this later). Chemigenetic sensors...

14. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...you are negotiating. Then take a moment before, during, or after a negotiation to think about the situation...

15. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...translated protein (sybody), ribosome, and mRNA. During the screening process, displayed sybodies that ...

16. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...mass bleaching, the loss of their symbiotic algae, during heat stress. For other organisms, like the sea ...

17. Transferable Skills Guide: Managing a Team

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    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...mine you didn’t think much about management skills during your scientific training. I never thought about...

18. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...their mental model of the protein. At some point during this interrogation process, they will almost invariably...

19. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...layered and then the viral suspension generated during AAV production is overlaid. Following ultracentrifugation...

20. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    Published

    Aug. 4, 2016, 2:30 p.m.

    ...protein to load an ssDNA guide. It is possible that, during translation, only a small fraction of nascent NgAgo...