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We narrowed to 694 results for: EED

Showing: 281 - 300 of 694 results

  1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...Across these methods, the following components are needed: γ-Retroviral transfer plasmid encoding a transgene...stably expresses Gag-Pol and/or Env, eliminating the need to deliver these genes in trans by specific plasmids...definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus. 3...

  2. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ... design phase of your experimental planning, you need more nitty-gritty details — how can I multiplex ...researchers, the previous information may be all you need to start your targeting experiment. However, many...many situations could arise where you still need more information. If you are performing a viral delivery...

  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...demystify all components of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types ...infiltrate and integrate into the host genome. First, it needs to be virally enveloped which is achieved by expression...matrix, capsid, and nucleocapsid viral components needed for viral packaging, assembly, and display. Pol...

  4. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...component, which we will refer to as the analyte, needs to be specifically recognized by another factor,...viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the tools). It’s...targetability. As mentioned prior, many biosensors need to be in specific tissues or cells to monitor their...

  5. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...capacity of ~ 8 kb, ample room for most experimental needs, and can transduce (infect) a wide range of cell...), an alternative virus, or other strategies may need to be considered if size constraints are an issue... cargo! Adenovirus is an effective option if you need high, transient expression of a genetic cargo or...

  6. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ... the individual variants found in tumors are exceedingly rare. As a result, how most of these “variants...But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit, the Broad...developing these assays, we figured that they would need to be generalizable and capable of measuring a variety...

  7. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...focused ... to understand cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile...tumor progression (Ref 2). Future treatments may need to target this immunosuppressive reaction in order...aspirin may give our immune system the boost it needs to help rid us of cancer in combination with chemotherapy...

  8. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... make it easier for you to find the plasmids you need. We'll be monitoring and improving the maps further...ability to find the plasmid with the features you need quickly. SnapGene’s Feature Library - SnapGene’...and quickly identify the plasmids and features you need for your next experiment. We would like to extend...

  9. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid... recently, the approach has been hampered by the need to tag each protein of interest and, for in vivo... the approach more accessible, as it reduces the need to generate “single-use” transgenic lines. Instead...

  10. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...recombineering strategies, only 3-4 phage proteins are needed and these can be provided via a separate plasmid...into stable circular plasmids - no nick repair is needed. Since the bacteria are subsequently grown at 37...Recombination efficiency is high enough to negate the need for selection usually required in recombineering...

  11. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...full-time and abandons all safety nets.” Raising seed money. “Before seeking funding, ask yourself if ...solid, and “the pitch is packaged to perfection”, seed money will hopefully be raised and the company may...Work fast. Be flexible. Be quick at failing and succeeding. Take home Starting a company requires dedication...

  12. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...selection markers in yeast and provides the element needed to overcome the auxotrophy as well as additional...means of selection: A specific selection marker needs to be used with a yeast strain deficient in that...element pairs must be utilized or a new combination needs to be created in advance of the experiment. The ...

  13. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...kb, meaning that your gene of interest (or genes) need to be 4.7 kb or less to fit in a single rAAV. For...gene of interest is truly small (<2.5Kb) and you need expression in a hurry, self-complementary AAV (scAAV...dsDNA in the nucleus and is a good option if you need rapid transgene expression, since you don’t have...

  14. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...your experiment? What about all the reagents you need to order? Sometimes, it feels like an eternity of... order or try to make it in your lab (but if you need a lot of plasmids, it’s almost always faster to ...right one can save you time if you’re working at speed or money if budget is a concern. The faster methods...

  15. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ... its normal neuronal context. In other words, I needed a method for visualizing protein turnover in situ...incorporation of puromycin into peptide chains." Proceedings of the National Academy of Sciences 51.4 (1964...puromycin of amino acid incorporation into protein." Proceedings of the National Academy of Sciences 45.12 (1959...

  16. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...channelrhodopsins in one region of the brain, which is needed since current channelrhodopsins tools have low ... tissue (~1 mm3). Current channelrhodopsins also need high-intensity light for activation which requires...transduction of the nervous system which eliminates the need for intracranial injections. When packaged in PHP.eB...

  17. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...CombiGEM-CRISPR experiments The first thing you will need is to get the list of effective gRNA sequences targeting... massively parallel combinatorial genetics." Proceedings of the National Academy of Sciences 111.34 (2014... CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the National Academy of Sciences113.9 (2016...

  18. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...underpinnings of neurological disease, neuroscientists need to be able to probe cells and circuits without disrupting...chemogenetic system To set up a chemogenetic system, you’ll need to choose a method for targeting receptor expression...cell types, or even subcellular regions of a neuron need to be targeted. Targeting is also important in the...

  19. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...mechanisms and, eventually, develop treatments, we need tools to specifically edit methylation sites.  Step... DNMT1 even through replication. The dCas9 also needs to be removeable, otherwise it will continue to ...nuclear environment. One good system to balance these needs is the lentiviral-based expression of floxed dCas9...
Showing: 281 - 300 of 694 results