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We narrowed to 924 results for: Gene

Showing: 281 - 300 of 924 results

  1. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...-based genome engineering has revolutionized the gene editing field by making experimental workflows considerably... tools. As a consequence, a critical step in the gene editing workflow - analyzing the data - is often...likely to result in a functional KO of the targeted gene.   Analyze Complex Edits   Traditional Sanger sequencing-based...result in a functional Knockout (KO) of the targeted gene. The analysis can be sorted by any of the parameters...validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please contact us at [email protected]... more efficient than previous methods. Still, generating reliable results from CRISPR edit data requires...detect or analyze complex edits, such as those generated by multiplexed delivery of multiple sgRNAs. Since...

  2. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...Additional resources on the Addgene blog Find out what makes AAV a versatile tool for gene expression in mammals...provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase, but... the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication; and 3)...a higher number of viral particles for efficient gene transfer. I figure it’s better to have a bit extra...derived inverted terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: 22428977...Resources on Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols...3) the transfer plasmid which encodes a transgene of interest that’s packaged into the virus. Two to five...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...insertions, deletions, and point mutagenesis to complex assembly of multiple genes. Primer design for each type...amplified, restriction digested or even a synthesized gene. PCR is the primary method to produce the linear...included in a single PCR, for example for subcloning genes from different templates. All PCRs are run as an... 5’ end of either primer. Subcloning - To move a gene from one vector to another simply amplify the vector...protein-coding sequence, or a mutation can be introduced in a gene, while adding a GFP-tag (Figure 3). Achieving multiple... to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis is performed by designing primers astride...using the same three step protocol:  Fragment generation - Any linear DNA fragments can be assembled in...

  4. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...provide an increased understanding of DNA binding and gene regulation. For additional information on CRISPR-mediated...technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed PMID: 26848818...interactions between genomic regions by enCh IP‐Seq." Genes to Cells22.6 (2017): 506-520. PubMed PMID: 28474362...the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells 23.4 (2018): 318-325. PubMed PMID: 29480524...RNA-guided platform for sequence-specific control of gene expression." Cell 152.5 (2013): 1173-1183. PubMed...Fujita et al., 2018). Furthermore, the Fujii Lab generated a transgenic mouse line and retroviral expression...specific gRNA-dCas9 complex. When combined with next-generation sequencing, this method allows for the recovery...

  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...where his group focuses on gene regulation in nematode molting and spermatogenesis. His group also builds ... one of the model organisms which I use to study gene regulation during developmental processes, CRISPR...Craig Mello’s lab – used inactivation of the unc-22 gene as their selection marker (Kim et al., 2014). Meanwhile... I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency (0.13%...yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi/CRISPRa), visualize specific...Gaul U, Forstemann K (2014) Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous...CRISPR toolbox for C. elegans: Cpf1 as an alternative gene editing system for AT-rich sequences https://doi.org...

  6. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2024, 1:15 p.m.
    ...Addgene is very excited to share the very first edition of our Antibodies 101 eBook! The latest addition... always, don’t hesitate to reach out to help@addgene.org with any thoughts, questions, or feedback. Happy...

  7. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...number of CRISPR libraries Addgene has distributed (1C), from 2012 to 2023. Addgene’s collection of CRISPR ...As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on...community. They did this by depositing them in Addgene’s repository. Other researchers, eager to try this... tools of their own to share. Over the years, Addgene’s repository has grown to include many CRISPR tools...This growth is supported by new labs joining the Addgene CRISPR community, with approximately 100 new CRISPR...CRISPR depositors each year.    Figure 1: Addgene CRISPR distribution and deposits. (A) Cumulative... (B) Cumulative CRISPR plasmids deposited with Addgene by year. (C) Cumulative number of laboratories ...

  8. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...media, you can find us on Bluesky: @addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and...we will not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated...an official Addgene account. You can reach out to us directly via email at help@addgene.org, and we invite...Addgene is announcing we have chosen to stop all activity on X, formerly Twitter. If you are interested...mainstay of conversation in the scientific community. Addgene has been considering a gradual move off X as a ...to paid content regardless of user preference. Addgene is a values-driven nonprofit. Recent remarks and...posting, monitoring, and resharing content on X.  Addgene deeply values our community and recognizes the ...

  9. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ... Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter their...hunting and interviews, I accepted a position at Addgene as a marketing and social media co-op. There are...realized I had made the right decision to choose Addgene for my co-op, but I know it didn’t take long. It... It may have been at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached...analytics, something that had never been done at Addgene before. This meant I was responsible for establishing...strategy. I really believe that the culture at Addgene cultivates trust between coworkers and that trust...retrospect, nonsensical. I have found the people at Addgene have become my friends, mentors, and some of my...

  10. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...from electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide ...cells expressing the optogenetic tool causing a change in cellular activity or gene expression. Experimental...activating the optogenetic tool, it can also cause problems when using multiple optogenetics or biosensors...information on hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide a powerful...! Since then, scientists have developed many optogenetic tools to control cellular activity with exquisite...exquisite precision using only light. Optogenetics, or light activated tools, can control everything from... post, we will discuss some broad classes of optogenetic tools, and what factors to consider when choosing...

  11. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...antibodies via Addgene to maximize the reach and impact of your work. Please email deposit@addgene.org if you ...Antibodies and Grow From There Addgene’s antibody resource is funded by a generous grant from the NIH’s Brain...reagents, share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If...Addgene is thrilled to announce the launch of our latest community resource: Recombinant Antibodies! ... your experiments. To promote reproducibility, Addgene will have unique Research Resource Identifiers ... preparations (Bradbury et al., 2015). All of Addgene’s antibodies are plasmid-based and recombinant. ...

  12. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    Published
    April 1, 2021, 1:15 p.m.
    ...You will be trying to find pictures of Addgene pets with Addgene-branded clothing on the blog. Below each...just fun and games this April Fool’s Day on the Addgene blog.  We’ve been mostly working remotely for the.... Now, we’ve collected photos of our pets with Addgene gear, and have unleashed them all over our recently...

  13. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ... also find information on using Addgene’s website, the latest Addgene news, and awards that we offer there...Say hello to the new Addgene blog! Same URL, same great articles to help you with your experiments, but...articles you want to find without even leaving the Addgene blog! Just click on the search bar at the top of...interview. Get in touch by emailing us at blog@addgene.org or tell us in the comments below. We’ll track...Be the first to know about new articles on the Addgene blog by subscribing to instant alerts below.   ...

  14. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ...their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s Science Video...change. Having been at Addgene for a month, I can say with certainty that Addgene is a company that cares...Sociology and Dance at Skidmore College. I was also Addgene’s Marketing Intern this summer! With the help of...made me intrigued by the marketing internship at Addgene. Although my interests are very clear, I had no...COVID was getting, I was hoping to walk into the Addgene office my first day and meet everyone but this ...Marketing Intern, I was tasked with continuing Addgene’s Meet an Addgenie Series on Instagram. I interviewed...project, I observed what it was like to work at Addgene. This is exemplified in my work interviewing fellow...

  15. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...all Addgene news Resources on Addgene.org Check out our job postings Learn more about Addgene's mission...about Addgene’s software development team and how we work.  Additional resources on the Addgene blog ...When you think of Addgene, chances are software development is not one of the things that comes to mind... is a lot of software development happening at Addgene under the hood. A significant portion of our development... benefits. We support everyone that works with Addgene in some way. For users, we just released a major...viral orders are being fulfilled more quickly. As Addgene grows and evolves, we will be here to increase ...development team and the development process at Addgene. Diversity supports our innovation Our software...

  16. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...blog@addgene.org.  Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos...Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience...decided to share some of our tips in our new series: Addgene Lab Tips! In each short episode of this series...Science: We Share Science Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol...

  17. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed nearly 2,400...deposited the associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these...associated with Addgene deposits accumulate more citations than those without Addgene deposits? Even more...with Addgene causes a paper to get cited more?   Articles with and without plasmids at Addgene To ...authors deposited with Addgene and those without plasmids deposited with Addgene. Together these journals...of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations So what... at Addgene – typically about four times as many as papers without plasmids deposited with Addgene (Figure...

  18. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Communications Manager Addgene's book club Indeed, reading is ingrained into Addgene’s culture. We have a ...still going on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product... of Addgene’s culture. Have you read any of these books? Whether you have or not, let the Addgene community...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene...and explains the origins of ABC: I founded the Addgene Book Club (aka ABC) in 2011 after having numerous... activities at Addgene ...

  19. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma...Developing the Addgene microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists...materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read Our ...The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see the ...with lots of researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors...dedicated to science communication through art. Addgene Advisory Board member Siouxsie Wiles gave us a ... about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...

  20. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... in most cases at Addgene, we check what we call the “insert,” (the gene or genetic element cloned into...information for a given plasmid are the backbone, inserted gene/functional mutations, antibiotic resistance, restriction... Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View our ...the best things about sharing plasmids through Addgene is that we provide an added level of confidence... plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we distribute...examples include Benchling, Serial Cloner, and Snapgene), and save these to a shared computer or server...physical sample and its associated information are at Addgene, we are ready to sequence verify the plasmids. ...
Showing: 281 - 300 of 924 results