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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 738 results for: HA

Showing: 281 - 300 of 738 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...pathways By Gabrielle Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting ...Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible...that is smaller and easier to deliver, but so far has few inhibitors identified.     New from the viral...

  2. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...available VSDs has been successful even at the single neuron level in vitro. In vivo monitoring has also been...biosensors, and generally as biological tools, our blog has you covered. Happy voltage sensing!   Figure...

  4. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ... of practice to ensure the proper injection site has been located and to avoid potential off-target pokes...researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing...: intermediate Your decision Hopefully this post has provided a good introduction to common injection ...

  5. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...DRSC/TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down...tools. The Vienna Drosophila Resource Center also has collections of flies for in vivo gene knock down ...finishing his immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila...

  6. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR ...of the sequence between the microhomologies.MMEJ has certain advantages over HDR. Some species don’t have...the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify due to NHEJ’s dominance ...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...expensive, it may be helpful to find an antibody that has already been validated for flow cytometry through...Characterization through Open Science) initiative has now started producing flow cytometry characterization...overexpression. Additionally, if the cell line used has endogenous levels of expression, this can make the...TIM-1 (Single pass type-1 membrane protein), which has been designated CD365 in the most recent HLDA workshop...as part of the YCharOS open science ecosystem. It has received in-kind support to produce this data from...

  8. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...cooperative education experience (co-op) is what has brought me, a second year bioengineering student,... that remote work would feel isolating, but that has not been the case for me at Addgene. There is a clear...

  9. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...manipulated, its anatomical origin, if cell line has ever been the target of an ‘-omics’ study or is resistant...can be used in some cases, but much of this work has required manually going through the pdfs of the 26,000...

  11. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...research and PhD studies, there lies a hidden gem that has the potential to redefine the course of one's research...inspiration. Additionally, journaling in the morning has proven invaluable for me, allowing me to pinpoint...

  12. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...bacteria in real time, but each of these reporters has its drawbacks. Acoustic reporter genes (ARGs) overcome... can colonize the mammalian intestinal tract and has been used for 100 years to clinically treat enteric...

  13. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary Ti vector for ...mosaic virus, the constitutively active M24 promoter has been modified with duplicated enhancer domains and...

  14. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...keynote speaker, Dr. Henry Bourne (shown in photo), has been speaking out and writing about issues in our...the same time, he pointed out, academic research has some big problems resulting from past practices and...

  16. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...research project can take several years to complete and has multiple benchmarks. Think about what your personal...Have a look at what others have done recently that has been a big hit, and especially look at the new tools...

  17. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...overexpression. Tim Formosa, at the University of Utah, has built a complete collection of yeast tagging modules...terminator-(selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site...

  18. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ... following IV injection. Compared to AAV9, PHP.B has a >40-fold increase in CNS transduction and transduces...adult mouse hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other Ly6 gene... line of viral vector permissiveness While PHP.B has impressive CNS transduction in some strains of mice...

  19. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...your research. RNA editing advantages RNA editing has multiple advantages over more traditional DNA editing...will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-...edited.   As discussed above, the ability to edit RNA has multiple advantages, including reversibility and ...

  20. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...months, COVID-19 has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused an estimated...contains requests from 5/22/14 to 5/19/20). Each paper has between 1–32 plasmids and a total of 2–2,590 requests...
Showing: 281 - 300 of 738 results