We narrowed to 405 results for: Lif;
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TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....gRNA library in plasmid format, a researcher must amplify the library, use next-generation sequencing to ...
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Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m.... 11 years he has studied various aspects extreme life on earth including extremophile microorganisms, ... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m....PID formats. For example, several journals like eLife, Nature, and Cell use Research Resource identifiers... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....conformational changes. These tiny movements are then amplified tens or hundreds of thousands of times by propagating... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m.... by controlled timing of CRISPR/Cas9 delivery." Elife 3 (2015): e04766. PubMed PMID: 25497837. PubMed ... -
Resume Writing for Non-academic Science Careers
TypeBlog PostPublishedDec. 18, 2014, 4:06 p.m....years. Does an academic career work well with your life and career plan? This might be a tough question ... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....fluorescent channels on the microscope. With the proliferation of fluorescent proteins in recent years, there... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral EBV or SV40... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....that those other components are still there in real life. Certainly referring to any DNA within the context... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....promoter and ceases repression of the target gene. A simplified lac inducible system is found in many bacterial... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m....scientists can start using Twitter and provide real-life examples of how they and others have used Twitter... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....explore a career in product management. A day in the life Now that you know a bit about what a software product... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....can be dissected at various times throughout the lifespan of the fly to determine the implications of your... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....and allele function via Cell Painting. Rohban MH, Elife, 2017. PubMed PMID: 28315521. PubMed Central PMCID... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m.... of enzymes: Library screening strategies. IUBMB Life, 61: 222-228 (2009). doi: https://doi.org/10.1002... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m.... the tag can recruit multiple frankenbodies to amplify the signal. Liu et al. Genes Cells. 2021. https... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....integration to express its cargo, in fact its viral life cycle does not include genomic integration. While... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....Janelia Research Campus and the University of California, Berkeley engineered an AAV variant that efficiently... -
Fluorescent Proteins 101: Luciferases
TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....chemical reaction takes place. Figure 2: Simplified chemical reaction catalyzed by luciferase, releasing...