We narrowed to 317 results for: MUT

1. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers...

2. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ...

3. Transferable Skills Guide: External Collaborations

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   Blog Post

   Published

   April 19, 2018, 1:25 p.m.

   ...leverage another organization’s expertise to our mutual benefit.  Our partnership with SnapGene, for example...

4. Viral Production at Addgene

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   Blog Post

   Published

   Oct. 24, 2023, 1:15 p.m.

   ...for viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids...

5. Viral Vectors 101: Preparing Pooled Libraries

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   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...transduction of cells, resulting in cell population mutated by the viral pooled library. These cells can then...

6. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ... This is to avoid the chances of insertional mutagenesis, which is the integration of transgenes into ...

7. Viral Vectors 101: Pseudotyping

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   Blog Post

   Published

   April 20, 2021, 2:30 p.m.

   ...2006) Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org...

8. Plasmids 101: Plasmid Incompatibility

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   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...plasmid replication control that are not necessarily mutually exclusive. In the first, the replication initiation...

9. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   Published

   Aug. 18, 2016, 2:30 p.m.

   ...upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity to a ...

10. The Challenges of Cell Culture

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    Blog Post

    Published

    May 5, 2016, 2:30 p.m.

    ...between cells lines, as this can be dependent on the mutation rate of the cell line, but it is broadly recommended...

11. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post

    Published

    Oct. 27, 2015, 3 p.m.

    ...fluorescent proteins and were re-engineered by mutating cysteine residues and N-glycosylation consensus...

12. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ... promoter with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus...

13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ... fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings of the...

14. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    Published

    March 5, 2019, 1:08 p.m.

    ...Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation...

15. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    Published

    Jan. 29, 2019, 2:27 p.m.

    ...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ...

16. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    Published

    Nov. 16, 2021, 2:15 p.m.

    ...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”...

17. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    Published

    June 3, 2021, 1:15 p.m.

    ...expression in strains of bacteria that are redox mutants so they have a more oxidizing cytoplasm. expression...

18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    Published

    June 17, 2025, 1:15 p.m.

    ... to knock down genes with potentially harmful mutations in therapeutic settings. Silencing of a target...

19. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    Published

    Sept. 29, 2020, 1:15 p.m.

    ...molecular tools, such as gene manipulation and mutagenesis techniques, are available to the scientific community...

20. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    Published

    Sept. 28, 2023, 1:15 p.m.

    ...damage other cellular structures, and genetic mutations are not easily reversible. Late last year, the...