We narrowed to 329 results for: NUC
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TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....traversing pons and medulla, bp= basilar pons, NST nucleus of the solitary tract. The arrows point the direction...
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Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m.... from transgenes to plants, chloroplast to the nucleus, or pathogens to their hosts. I spoke with her ... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....with SRM techniques, and adapted for detecting nucleic acids as well as proteins (Klimas, et al. 2023;... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....African descent have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....cell type, even within the same brain region or nucleus. Just optimizing AAV expression can take months... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....fluorophores were designed as antibody and oligonucleotide labels for fixed-cell imaging, not for live-cell... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....think of DNA as a linear polymer of A,T,G, and C nucleotides arranged in a double helix. People are wrong.... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m.... cloning by homologous recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID:... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....because they remain in episomal form in the cell’s nucleus. But since nothing’s ever perfect, neither is ... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....in 5). The RNA genome is encapsidated by the nucleoprotein, NP. NP associates with the viral polymerase... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m.... with a tag that localizes your protein to the nucleus? Or you want multiple rounds of purification to... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....retroviruses. Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing ... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....scatter roughly measures granularity, a proxy for nucleic complexity of the cell. The sample will have been... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m.... able to label proteins moving to and from the nucleus, ER, mitochondria, stress granules, and more. For... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....have accumulated more than 300,000 individual nucleotide sequences. More than half of these sequences ... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....DnaA-ATP in initiation of chromosome replication. Nucleic Acids Research, 46(12), 6140–6151. https://doi.... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...