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We narrowed to 644 results for: Rest

Showing: 281 - 300 of 644 results

  1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...first developed in the 1990s. While traditional restriction enzyme cloning uses short sticky ends to insert.... To start the SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology regions...multiple fragments without the need for specific restriction sites or DNA ligase! This post was originally...

  2. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...been hampered by the need to tag each protein of interest and, for in vivo work, generate a dedicated transgenic...GFP-nanobody rather than a specific protein of interest. This fusion protein can then be co-expressed ...expressing a GFP-tagged version of a protein of interest. The binding of the GFP nanobody to its target...

  3. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...activated B cell that produces the antibody of interest with a myeloma cell. This RNA was then used to...Constructs were then verified by both diagnostic restriction digest and colony PCR.   Fig. 1: (A) ... found that the recognition sequence for the restriction enzyme BciVI was present in the aberrant light...

  4. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...efficiency. However, gamma-retrovirus infection is restricted to dividing cells only, so is not suitable for...can target the virus to specific cell types of interest. This is a particularly nice feature as organ-...have an idea which ones could be helpful to you. Interested in learning more about a specific virus? We have...

  5. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...coli, carrying a plasmid expressing a protein of interest). Mesophilic microbes are highly preferable (and...delighted to engage with potential collaborators and interested audiences. Please visit our team wiki or contact...at the iGEM Competition 2019. He has an active interest in scientific writing and science communication...

  6. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...Researchers express genes of interest from plasmids in order to study gene function or to engineer cells...fluctuations due to copy number by introducing genes of interest into the chromosome. But this has its own set ...thereby intensifying repression of the gene of interest and keeping its expression level constant. In ...

  7. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...doing after the injunction? We are particularly interested in whether institutions planning to raise salaries...our FOIA requests, we are starting to see very interesting data on how much postdocs are actually getting...Executive Director of Future of Research. He is interested in advocating for and with junior scientists,...

  8. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...Health Science Center at Houston. Prakash became interested in studying TS when he was studying thoracic ... Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when I was a newly independent faculty ...channels in the brain.  “I have a long standing interest in the biology of GPCRs and G proteins, with an...

  9. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...fail. Our laboratory manager would definitely be interested to see which part of her procedures are the most...getting up and running and more time working on interesting biotechnology code.There are valid technical ...Shamloo is a Software Engineer at Addgene and is interested in writing software tools to help scientists....

  12. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...in plants to conditionally deplete proteins of interest in C. elegans (Zhang et al., 2015). In this system...spatially and temporally knock-down proteins of interest.  Plasmids for cGAL and split cGAL systems The...halves of cGAL can drive expression of your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene...

  13. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...which may be of particular interest to MoClo users!). If you’re interested in complex Golden Gate Assembly...overhang set by uploading the set with the Type IIS restriction enzyme and thermocycler protocol. It will also...

  14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...potentially signaling a rough road ahead for students interested in leading the process despite the recent advance...students will pursue this newly available avenue. Interestingly, both of these changes share a common thread...understand human disease.  Outside the lab, he is interested in communicating science and in the intersection...

  15. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...integrity of your virus, perform a diagnostic restriction enzyme digest and compare it to some reliable... use the virus immediately upon harvesting. Interestingly, freeze-thawing host cells immediately prior...24003179. PubMed Central PMCID: PMC3760253. 2. Rohwer, Forest. "Global phage diversity." Cell 113.2 (2003): 141...

  16. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...and type of target sites that flox your genes of interest, a powerful FLEx switch can be created for your... considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position...can be employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used...

  17. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...chance as entrepreneurs. To make things more interesting, we are working in a new technical discipline...things we could do for work, and they weren’t restricted to science jobs. Keira’s an optimist, I’m a realist...doing makes sense. I can support that.” That was interesting. If people could be against GMOs as food but ...

  18. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...gradients use dilutions of your sample or protein of interest to understand the dynamic or working range of ...particularly in the sizes around your protein of interest, but you don’t want to run so long your dye front...membrane. If you’re not sure if your protein of interest is over-transferring (going completely through... validated for your protein and application of interest or validating an antibody yourself. But even with...

  19. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...transferred from B. subtilis to the bacterium of interest following IPTG induction of conjugation genes ... transferred to a recipient bacteria strain of interest by tri-parental mating. Donor bacteria contain...ready to move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...

  20. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...with your transfer plasmid, carrying your gene of interest, and the two helper plasmids, commonly called ...Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process, which is what ...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...
Showing: 281 - 300 of 644 results