We narrowed to 347 results for: SAM-2

...contain the expected plasmid features.  Figure 2. Addgene’s basic QC process. Created with BioRender.com...ensure the entire team is using the same rubric and making the same decisions when analyzing the wide variety... that is provided, each deposit goes through the same QC process to ensure all plasmids pass our high-...

... enrich the microvesicles containing AAVs (Figure 2). Since the development of the exo-AAV isolation protocol... have a higher transduction efficiency, i.e. the same number of AAV particles is able to infect more cells...

... 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3, CHO, 293HEK... for cells that have taken up the plasmid in the same way. Selection methods, described below, are typically...

...to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched recombinant...detect multiple targets with different mAbs in the same experiment. The Trimmer Lab found that they could...

...aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...suggests a change to a slide, think about whether the same principle applies to other slides in your deck.  ...

...improve the brightness of previous Nano-lanterns by 2-4 fold and permit five-color fluorescent imaging! ...external light source. Therefore, especially thick samples may not be appropriate for use with Nano-lantern...

...promising monoclonal antibody with high specificity. (2) Narrow down the specific epitope that your antibody...between endogenous and exogenous versions of the same target. In the 1980s, Munro and Pelham set out to... ideal.  Unexpected connections Right around the same time that Munro and Pelham were devising their tagging...from this year.  But are we all really using the same antibody developed almost 40 years ago? Well…kinda...distributing monoclonal antibodies derived from the same hybridoma developed by Evans, et al. In other cases...

...al (Nature. 1988 Jul 28;334(6180):320-5.) PubMed. 2. A segment of the 5' nontranslated region of encephalomyocarditis...simultaneously express two or more separate proteins from the same mRNA. We've discussed promoters before so in this...expressing your gene as they are both derived from the same transcript. Of course multicistronic vectors do ...anytime you want to express multiple genes in the same cell. Although it is possible to drive co-expression...expression cassettes, having the genes expressed from the same cassette is sometimes advantageous, particularly...produce equimolar levels of mulitple genes from the same mRNA. The term "self-cleaving" is not entirely accurate...successfully utilized 2A and IRES elements within the same multicistronic vector, effectively making a construct...

...rate of 10-55% - the PASTE system.     Fig. 2: Schematic of programmable gene insertion with PASTE...lab often tests samples of new bacteria from anywhere they can find, whether the samples are from a rain...was safe to say the sample had their attention! They isolated DNA from the sample, amplified the CRISPR...range of mammalian cell lines. However, in those same comparisons, the AbuGoot lab reported no detectable...thousands of different mutations, all found in the same gene. If we only have small, directed editing abilities...

...divided by the off-target activity. Figure 2: Specificity is the measure of off-target activity...NGG, NGAN, NGNG, NGAG, and NGCG). Since then, the Osamu Nureki Lab described a rationally engineered Cas9...

...Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig...low immunogenicity, meaning it does not induce the same high immune response as retroviruses. This virus...

...PubMed PMID: 8491198. PubMed Central PMCID: PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human...designed to make you sick…well, you feel it. But the same features that make these familiar viruses such a...

... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware...very difficult to adapt the device to one's own samples and limits the use of microscopy in open science...

...and are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for...modify proteins yet they still share many of the same technical advantages that come with working with...

...PMID: 20543841. PubMed Central PMCID: PMC2920597. 2. Nathans, Daniel. "Puromycin inhibition of protein... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific...

...don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same three nucleotides...in a row; (4) no more than two nucleotides in the same position; (5) avoid overhangs with either 0% or ...71% (Pryor et al., 2020). And in 2022, using the same tools, they successfully assembled a 40 kb T7 phage...

...GUS-staining is a very useful visual reporter (Figure 2). The GUS enzyme cleaves a substrate, which will then...thanks to our guest blogger Samuel Mortensen from Northeastern University. Samuel Mortensen is a PhD candidate...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with...19457242. PubMed Central PMCID: PMC2693113. Mortensen, Samuel, et al. "EASI transformation: An efficient transient...

... 2008 Dec; 18(6): 617–623. Pubmed PMID 19349161.  2. Deshpande A, Bergami M, Ghanem A, Conzelmann KK, ...necessary and sufficient for retrograde transmission (Etessami et al., 2000, Wickersham et al., 2007). Thus, ...Mar 19;110(12):E1152-61 Pubmed PMID 23487772. 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, ...

...work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life Sometimes...whatever works for you, but most days go home at the same time. Use time management tools and tactics to plan...need to match up exactly when you fold them. The same with sheets – wash them and put them right back ...family by forming a social circle of people in the same situation. Go to church, join a club, meet the neighbors...

...PMID: 24901218. PubMed Central PMCID: PMC4047077. 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata ... this purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the...