We narrowed to 342 results for: SEF;

1. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   .... While the physical titer generated by qPCR is useful, I test a few different concentrations of virus...

2. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

   Type

   Blog Post

   Published

   Oct. 11, 2018, 12:30 p.m.

   ...assembly reactions in which a single component was purposefully omitted. Since any assembly is dependent on ...

3. Transferable Skills: Negotiation

   Type

   Blog Post

   Published

   March 3, 2022, 2 p.m.

   ...for opinions from a neutral third party can be a useful strategy to help get unstuck and move closer to...

4. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   Published

   July 2, 2020, 12:40 p.m.

   ...pictures” generated with current 3C methods provide useful information about chromatin interactions, they ...

5. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ...These tools are available at Addgene and could be useful to labs wishing to create this kind of animal models...

6. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...differentiate into multiple cell types makes them useful models for developmental processes and promising...

7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...gRNA in a wide variety of mammalian cell lines. Useful for difficult to transfect cell types and can be...

8. Great Results Start with Great Standard Curves

   Type

   Blog Post

   Published

   Aug. 15, 2024, 1:15 p.m.

   ...data points generated by replicate values provides useful information about the accuracy of the curve, which...

9. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ...sensitivity of the assay. Where possible, it can be useful to compare recombinant antibodies to their parental...

10. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning. MXS Chaining...

11. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ...Wu et al., 1997) inside of the capsid . This is useful for viral reproduction, and for causing contagious...

12. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ...while using less resources and are particularly useful when trying to screen a limited number of cells...

13. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...protein degradation pathways. This makes Cas9 RNPs useful for CRISPR applications where limited expression...

14. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...subclasses and different species, nanobodies are useful for colocalization labeling of up to 3 targets ...

15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...the dissociation of PIPs. Thus, whereas BiFC is useful in scenarios that call for a simple, yes-no answer...

16. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...experiences in graduate school. These skills will be useful anywhere I work in the future; they are transferrable...

17. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...in the real world, coordinates alone aren’t very useful. We’ll need something like Google Maps to help ...

18. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus...

19. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...regardless of which is your favorite, colony PCR is a useful tool to have in your molecular biology tool kit...

20. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ... to insolubility, and aggregated protein is not useful protein. MBP tags can help with solubility issues...