We narrowed to 399 results for: SEF;

...Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources can be found below: ...and track clones of cells over time. Examples of useful tools for studying cells that share clonal origin...Protein Fusion Construct Set - Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein...

...Cre , Flp , Dre , other recombinases , as well as useful empty backbones or reporters . Experimental Considerations...event. Toxicity Cre-lox recombination is a very useful tool, but Cre expression can be toxic in some systems...undergone recombination. Reporter plasmids can also be useful for lineage tracing, evaluating infection/transfection...

...luciferase have short protein half-lives, making them useful as transcriptional reporters, but are less-than-ideal...an illumination source. BRET can be particularly useful for in vivo imaging or creating biosensors. Read... And see PalmGRET , a membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet...

...transfect. However, note that this method is most useful for cell lines that you can transfect at high efficiency...types by using cell-specific promoters, which is useful for in vivo studies. While we don’t discuss CRISPR...population. Additionally, clonal populations are useful for determining if your edit is homozygous or heterozygous...

... to being useful for our bodies to fight off intruders, antibodies are also extremely useful to researchers...

... resources for the community by depositing your useful tools , or by suggesting plasmids for the collection...constructs for endogenous tagging. Some examples of useful worm CRISPR resources, along with links to protocols...

...tags such as NanoLuc, HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins...endogenous expression levels. NanoLuc fusions are useful for both intracellular and extracellular applications...

...have a map and know exactly what it should be. A useful restriction enzyme based technique for verifying...

...minipreps and RNA extractions. Plus, centrifuges are useful for simply collecting liquid to the bottom of a...

...Page . We hope that you'll find these protocols useful in your own work. Please feel free to email us ...

...together several oligos. This technique is especially useful for introducing promoters, terminators, and other...

...gene of interest in a target cell line and can be useful for short term studies of protein function. We ...

...cells. This is a relatively simple procedure and is useful for performing low efficiency transformations. ...

...Background due to vector re-circularization - most useful for phosphatase treated vector Insert or water ...

...Find materials useful for neurodegeneration research....related disorders. The site curates information on useful resources such as: Antibodies (Link opens in a ...

...The Rinehart Lab has developed several tools useful for the incorporation of phosphoserine into your ...library (Pooled Library #111704) is anticipated to be useful for laboratories performing phosphoproteomic experiments...

...previously acquired skills, notably loss of acquired purposeful hand movements and regression of speech. In contrast...syndrome of autism, dementia, ataxia, and loss of purposeful hand use in girls: Rett’s syndrome: report of...

...correct orientation in the recipient plasmid, it is useful to see if one set of enzymes will work in the same...

... constant regions of other antibodies. This has useful research applications, and is covered in the following...environmentally stable and more cost-effective. They are useful when epitope specificity is not a concern or in...

...Cell Migration Consortium have deposited plasmids useful for cell migration research at Addgene. CMC investigators...