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Showing: 281 - 300 of 573 results

  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...

  2. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ... helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The ...purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of ...

  3. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ... change across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials...detailed below. Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to...

  4. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ... tends to go something like this:   However, as simply as it seems laid out above, there are many places...potential programs and universities stressful or simply not feasible. Through the virtual systems created...

  5. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...End point PCR, which is commonly referred to as simply PCR, is used for an array of different molecular... or pathogen identification. Scientists can simultaneously detect several different pathogens in one specimen...

  6. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast bioproduction pathways... a reaction pathway, the MPyV system provides a simple, efficient method for protecting a single enzyme...

  8. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...after transforming them into your cells, you can simply look for blue colonies to determine which cells...cost yet. However, many fluorescent proteins face similar limitations, and many labs are already using these...

  9. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening ... CRISPR/Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR...

  10. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...figure below). The double-MMEJ strategy looks very similar to HDR, but it is accomplished using much smaller... of MMEJ. This double MMEJ strategy looks very similar to HDR, but it is completed using much shorter ...

  11. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...Pooled lentiviral CRISPR libraries (referred to simply as “CRISPR libraries”) consist of a heterogeneous...

  12. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from ...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

  13. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...forward-engineering the central dogma across many genes simultaneously. These parts have been carefully engineered...incredible hidden story here. The parts we are releasing simply plug into this existing economic activity. People...

  14. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated... mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by running ...

  15. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...only the linear portion. This allows you to use a simple linear regression trendline, which may be good ...

  17. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...non-scientist friends and family is Part Five, called simply “Bad Science.” Zimmer kicks off the section saying...provides several. In Part Three of the book, called simply “Old Science,” he describes the now infamous tale...

  18. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...to allow those who could not attend a meeting to simply stay caught up and to allow those who may be interested...own knowledge of the field. Perhaps interest has simply waned. When attendance slumps below a certain level...

  19. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...between 150-75 nucleotides upstream of the TSS. Similar to the Brunello and Dolcetto library, sgRNAs were...

  20. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... insert and one primer anneals to the insert. A simple way to create this type of primer pair is to mix-and-match...  Primer Design Pros Cons Insert-Specific Simple "yes or no" test results Doesn't tell you the...
Showing: 281 - 300 of 573 results