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Showing: 281 - 300 of 339 results

  1. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...range of the assay for a wide range of titers. We usually dilute our samples 1:6 million to 1:25 million....

  2. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...sequences are not easy to amplify by PCR, so we usually add DMSO (3%) and Betaine (1 M) to the PCR mix....

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...transcripts). A different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used...

  5. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the...

  6. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...potential generation of replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through...

  8. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...Finally, you can sound credible and cool when you casually mention “Haery et al., showed that MYC expression...

  13. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...Nanobodies are smaller in size, more durable, and usually much easier to clone, express, and purify compared...

  15. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...feature as done correctly. These initial stories are usually a result of collaborations between Addgene’s Product...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...Pandas, Numpy, and Biopython.  We started at the usual recommended place: documentation. We oriented ourselves...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...few scientists state that they’re not creative, usually saying that they’re not artists, or artistic, and...
Showing: 281 - 300 of 339 results