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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 351 results for: SUA

Showing: 281 - 300 of 351 results

  2. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...transcripts). A different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used...

  4. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...range of the assay for a wide range of titers. We usually dilute our samples 1:6 million to 1:25 million....

  5. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...sequences are not easy to amplify by PCR, so we usually add DMSO (3%) and Betaine (1 M) to the PCR mix....

  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...characterized and found to suffer from early and severe visual impairment that faithfully recapitulated the human...

  9. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...these components, one can generate constructs to visualize cell cycle dynamics, titrate inducible transgene...

  11. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...Damstra HG, Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue ultrastructure using Ten-...

  12. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...relies on repair from a template molecule, which is usually an endogenously available sister chromatid, though...

  13. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the...

  14. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...potential generation of replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through...

  16. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...raw data.  Addgene’s de novo assembly pipeline usually generates the correct plasmid sequence. However...

  18. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...Pandas, Numpy, and Biopython.  We started at the usual recommended place: documentation. We oriented ourselves...

  19. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...few scientists state that they’re not creative, usually saying that they’re not artists, or artistic, and...
Showing: 281 - 300 of 351 results