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Addgene

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We narrowed to 512 results for: Set

Showing: 281 - 300 of 512 results

  2. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...especially for systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...profiles have limited their usability in clinical settings. On the other hand, vectors derived from AAV have...the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until...

  8. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ... brightness is compared to that of EGFP which is set as 1. Some proteins are very dim (e.g. TagRFP657,...

  12. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...

  13. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... notice for the first meeting so that people can set aside time in their schedules and read the paper ...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...

  16. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... you identify bacterial and yeast contaminants. Set up a routine to split cells Don’t be lazy about splitting...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...allow for two sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus...protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a stop codon...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary for...for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA...creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific gRNA cleaves ...and 3’ microhomologies are added to the GFP-Puro cassette via PCR, and this construct is inserted into the...

  20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ... the use of a given timer in many experimental settings. Monomeric fluorescent protein timers Since the... simplifies cloning and makes your experimental setup less cumbersome. 3. Portability across multiple ...
Showing: 281 - 300 of 512 results