We narrowed to 520 results for: Set
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  TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....sample, we’ll then load it on to our new MiSeq and set up the run making sure to note the appropriate barcode...
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  Adeno Associated Virus (AAV) for Cell and Gene TherapyTypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....profiles have limited their usability in clinical settings. On the other hand, vectors derived from AAV have...the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until...
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  Plasmids 101: Knockout/Knock-In PlasmidsTypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....case, you design your targeting vector such that a set of three LoxP sites flank the resistance gene and...targeted gene. Upon recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted...sequence flanked by LoxP sites while the resistance cassette has been placed into an intron. Figure...
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  6 Tips for Grant WritingTypeBlog PostPublishedOct. 18, 2016, 2:30 p.m....Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you can ...
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  Savvy Advocates Needed to Navigate a Scientific Enterprise in FluxTypeBlog PostPublishedSept. 13, 2016, 2:30 p.m....recommended pay scale – used by many institutions to set postdoc wages. The second event of note is a ruling...Assistant Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology...
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  Plasmids 101: Terminators and PolyA signalsTypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....read-through. Chris Voigt’s lab has characterized a set of prokaryotic terminators and deposited several ...
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  The Materials Science of Optogenetics ExperimentsTypeBlog PostPublishedSept. 17, 2015, 2:30 p.m....of several lasers simultaneously, can be used to set the laser output without manual manipulation of the...will open but will not be given an opportunity to “reset” and become receptive to light once again. Therefore...
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  Genome-wide Screening Using CRISPRTypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....heterogeneous population of mutant cells, with each cell or set of cells containing a mutation in a different gene...
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  7 Simple Strategies to Resolve Conflicts with Difficult SupervisorsTypeBlog PostPublishedJan. 21, 2015, 1:30 p.m....supervisor, prioritize your action item list and set some approximate timelines. Be sure that you keep...
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  Sharing Your Lab Protocols: Using Apps to Save Time & Track Your ExperimentsTypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....steps in a protocol as you finish them, turn on pre-set timers for incubation steps, and save the entire ...
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  10 Basic tips for mammalian cell cultureTypeBlog PostPublishedJuly 12, 2018, 1:09 p.m.... you identify bacterial and yeast contaminants. Set up a routine to split cells Don’t be lazy about splitting...
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  The Scientific Conference Poster Session: Tips for SuccessTypeBlog PostPublishedAug. 29, 2019, 12:50 p.m....list of posters of interest, then you’ve already set yourself up for success. But sometimes, attendees...
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  Capturing a Moment with Marc Zimmer’s “The State of Science”TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....future in under 200 pages is an impressive task to set oneself. As a scientist who enjoys learning a little...
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  Meet an Addgenie: Insights into Grad School, Career Paths, and MoreTypeBlog PostPublishedJune 25, 2020, 1:15 p.m....have the idea that if you don’t get your PhD you’re set up for failure. Listening to Kenkel’s story has helped...
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  No Llamas Required - Synthetic Nanobodies Against Membrane ProteinsTypeBlog PostPublishedJune 18, 2020, 1:15 p.m....convex scaffold and are randomized with a defined set of different trinucleotides (corresponding to different...
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  AAV Vector Quality Control: Going the Extra Mile with NGSTypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....a large number of hits to one sequence/gene - we set our threshold at >100 hits - immediately raises a...
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  Cloning Mammalian Cells with the Agarose MethodTypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....containing serum in each well. A 200 ul pipettor is set to 200 ul, wiped down with 70% ethanol, and a box...
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  Of Myc and MenTypeBlog PostPublishedJan. 19, 2023, 2:15 p.m.... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...
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  A Guide to Starting Your Own Journal ClubTypeBlog PostPublishedFeb. 6, 2020, 3 p.m.... notice for the first meeting so that people can set aside time in their schedules and read the paper ...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...
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  Chromoproteins: Colorful Proteins For Molecular Biology ExperimentsTypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....chromoproteins are actually a subset of the fluorescent protein family. What sets them apart is the way they...