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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 612 results for: Sis

Showing: 281 - 300 of 612 results

  1. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...genes as drivers of peritoneal mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids...peritoneal mesothelioma cells lines and assess their tumorigenesis. These experiments will serve to validate these...

  2. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...they also went in search of genes involved in resistance to the cancer drug vemurafenib. Enriched libraries... screen for genes involved in bacterial toxin resistance, identifying 4 previously unknown genes.  “With...

  3. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ... evolution took over (with a little mutagenic assistance). Quicker CAST activity allowed certain mutated...evoCAST to the Liu lab’s own PASSIGE (Prime-Editing-Assisted Site-Specific Integrase Gene Editing), which combines...

  4. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

  5. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...  Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter ...presentation.  My first time in the lab! I got to assist production on a protocol video. Don't worry - no...

  6. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...microtubules. Tanenbaum et al. then tested their hypothesis that the lower expression levels of SunTag constructs...suitability in your experiments on a protein-by-protein basis. However, since many of Addgene’s SunTag plasmids...

  8. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...perception of head injury risk. In completing a master’s thesis in science communication, I had the chance to carry...necessary to inform the experimental aspect of my thesis: a survey instrument designed to measure how individuals...

  9. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...Instead, it targets a specific audience - giving consistency, purpose, structure, and intent to the messaging...your audience, and their expectations of you, consistent and clear.   And now that we’re all on the same...

  10. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...detection of the tagged protein. These peptides consist of as little as 8 or as many as several hundred...10.1007/s00253-002-1158-6 Baker, M., Reproducibility crisis: Blame it on the antibodies. Nature (2015) 521,...

  12. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...neuronal circuit tracing.     Marcus Leiwe is an assistant professor at Kyushu University. He has a particular...resolution using chemical cocktails and computational analysis." Cell 157.3 (2014): 726-739. PubMed PMID: 24746791...

  13. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ... dynamic process which involves autophagosome synthesis, delivery of materials to be degraded to the lysosome...mammalian dual-emission autophagy biosensor. Its resistance to lysosomal proteases is key to its pH-dependent...

  15. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ..., 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic element of...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12...

  16. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...degree and some marketing experience, with little emphasis placed on the marketing or business background...thought they would focus on “Has this person been consistent throughout their career?”, "Have they sought ...

  17. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...activity to start a discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring...series of mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer Mentor Groups Guide", "Tips...

  18. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...beginning makes the sharing process smoother, more consistent, and more pleasant for everyone, which in turn...including MTAs, is perfect, but it does provide consistency and transparency in an otherwise messy process...
Showing: 281 - 300 of 612 results