We narrowed to 608 results for: Sis;
-
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...
-
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog PostPublishedJune 29, 2023, 7 p.m.... Joining the Addgene crew! This pattern was consistent: everyone I spoke with at Addgene, no matter ...presentation. My first time in the lab! I got to assist production on a protocol video. Don't worry - no... -
SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....microtubules. Tanenbaum et al. then tested their hypothesis that the lower expression levels of SunTag constructs...suitability in your experiments on a protein-by-protein basis. However, since many of Addgene’s SunTag plasmids... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....Optogenetic Tools Like pDawn Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson, University... cry genes from the soil bacterium Bacillus thuringiensis and analysing the insecticidal potential of ... -
Antibodies 101: Epitope Tags
TypeBlog PostPublishedMay 11, 2023, 1:15 p.m....detection of the tagged protein. These peptides consist of as little as 8 or as many as several hundred...10.1007/s00253-002-1158-6 Baker, M., Reproducibility crisis: Blame it on the antibodies. Nature (2015) 521,... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....neuronal circuit tracing. Marcus Leiwe is an assistant professor at Kyushu University. He has a particular...resolution using chemical cocktails and computational analysis." Cell 157.3 (2014): 726-739. PubMed PMID: 24746791... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m.... dynamic process which involves autophagosome synthesis, delivery of materials to be degraded to the lysosome...mammalian dual-emission autophagy biosensor. Its resistance to lysosomal proteases is key to its pH-dependent... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....We believe that this simplified sequencing and analysis approach can be easily incorporated into standard... A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where Zdk1 binds...RNA localization in mammalian cells. These tools consist of cobalamin-binding riboswitches that bind to ... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m...., 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic element of...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m....perception of head injury risk. In completing a master’s thesis in science communication, I had the chance to carry...necessary to inform the experimental aspect of my thesis: a survey instrument designed to measure how individuals... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....Instead, it targets a specific audience - giving consistency, purpose, structure, and intent to the messaging...your audience, and their expectations of you, consistent and clear. And now that we’re all on the same... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....degree and some marketing experience, with little emphasis placed on the marketing or business background...thought they would focus on “Has this person been consistent throughout their career?”, "Have they sought ... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....activity to start a discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring...series of mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer Mentor Groups Guide", "Tips... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....beginning makes the sharing process smoother, more consistent, and more pleasant for everyone, which in turn...including MTAs, is perfect, but it does provide consistency and transparency in an otherwise messy process... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....controlling for journal. And so, while our quantitative analysis cannot rule out all other explanations, we do ...ssrn.com/author=1232466. Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT... -
Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....Ecologist Computer and writing skills Writing a thesis or dissertation demonstrates that you have advanced...what each of these roles involves on a dad-to-day basis. Often, it is hard to know what a job title actually... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased... plant surfaces. His system of choice is the Arabidopsis thaliana - Pantoea agglomerans interaction. But... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog PostPublishedApril 18, 2019, 12:53 p.m....visualized and results were consistent with copy number variation (CNV) analysis of the U2OS cells. ... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....2017. Pubmed PMID: 16371404 Sniderman et al, Athersclerosis 2012. Pubmed PMID: 23068583 Thierer et al, ...UAS). The Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif...