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We narrowed to 458 results for: Spr

Showing: 281 - 300 of 458 results

  1. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ..., they eliminate the expression of the gene (by CRISPR knockout, shRNA depletion, etc.) and ask what the...e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007...

  2. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...with the Addgenies on March 11, 2020 after rumors spread in our building that someone from a different company...enough funds to make most of our payroll for the spring and early summer months. We are also extremely ...

  4. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ....cell.2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and transcriptional activation screening...Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library  ...

  5. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ..., our journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People ...Blog Experimenting in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science...

  6. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org Browse Pooled...

  8. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... of this far-red/red light-responsive system is spread across three plasmids, with the response regulator...limited by a few factors. These bulky systems were spread over a number of plasmids, with one RR (ompR) encoded...

  9. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    .... The continued development of more and smaller CRISPR tools is an exciting area for future AAV application...- a primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources ...

  11. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...Discuss Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...

  12. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles...

  13. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...should be come available in the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now...now available as a lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human...

  14. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et...genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging...

  15. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...Save Time and Track Your Experiments Try out the CRISPR Software Matchmaker Additional Resources on Addgene.org...Protocols for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...

  16. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...needs to be added to media after autoclaving. If spread on top of pre-made plates, make sure it is evenly...Although blue-white screening is probably the most widespread way to select for plasmids containing an insert...

  19. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...

  20. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...happening within the keratinocytes. “We hope to use CRISPR libraries from Addgene to set up assays to see ... contraction defects. The lab also plans to use CRISPR plasmids to generate deletions of candidate genes...
Showing: 281 - 300 of 458 results