We narrowed to 323 results for: Stil

1. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...Don’t want to permanently modify the genome but still want to affect gene expression? dCas9 can be used...

2. Bioinformatics at Addgene

   Type

   Blog Post

   Published

   July 14, 2022, 1:15 p.m.

   ...processing of our sequencing data. However, our work still wasn’t complete. In order for our QC team to smoothly...

3. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ...faster, and more efficient than previous methods. Still, generating reliable results from CRISPR edit data...

4. AAV Titers: Where Do They Come From And What Do They Mean?

   Type

   Blog Post

   Published

   Nov. 15, 2017, 3 p.m.

   ...do our best to send you a particular lot (if we still have it available). References 1. Fagone, Paolo...

5. Career Insights: Technical Support Specialist

   Type

   Blog Post

   Published

   Nov. 9, 2017, 2 p.m.

   ...prepares one for numerous non-academic careers. Still, many start their scientific careers hoping to follow...

6. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre recombinase. FLPe codon ...

7. Plasmids 101: Common Lab E. coli Strains

   Type

   Blog Post

   Published

   Nov. 7, 2014, 2:56 p.m.

   ...hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated...

8. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   ...negotiations can be much easier. However, negotiations can still go horribly awry when several factors aren’t taken...

9. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   Published

   April 24, 2015, 2:39 p.m.

   ... models, and new Cre-lox based technologies are still being developed today. In this post, I will highlight...

10. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...). If so, the nicking mutagenesis protocol will still work if the orientation of the BbvCI site(s) of ...

11. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ...following gates, cells that are not of interest can still be gated out later. Based on personal preference...

12. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...customer support, but there are eight other teams who still retain a customer support element amongst their ...

13. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    Published

    Nov. 16, 2021, 2:15 p.m.

    ...Even with all of the resources available, it may still be difficult to find an antibody in which you are...

14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...GFP-like probes by supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional...

15. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ...are packaged, the physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene...

16. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...that have only gone through the single cross over still contain sacB and will not grow on sucrose. One ...

17. Transferable Skills Guide: Time Management

    Type

    Blog Post

    Published

    Dec. 5, 2017, 1:52 p.m.

    ...option. Even if the full experiment fails, you’ll still have made small accomplishments each day that should...

18. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...research in a lab in California. I felt then, and still feel now, that it is important to take advantage...

19. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ...common problems that many others have faced yet, still, this challenge did not hinder them from advancing...

20. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ... embryonic development and cancer. But there is still much to learn about their myriad functions and therapeutic...