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We narrowed to 693 results for: Tec

Showing: 281 - 300 of 693 results

  1. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening to microbes...vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression ... high spatial resolution are benefits of this technique. It is even possible to multiplex ultrasound dyes...

  2. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...blog post, keeping up with the newest CRISPR technologies and their applications can be exhausting. A ...addition to mutations in the spacer itself, will protect the HR template from the Cas9. Again, if it's within...of the Cong et. al (2013) Science paper). If you detect positive HR, isolate single-cell colonies, grow...editing, regulating and targeting genomes. Nature Biotechnology, 32(4), 347–355. https://doi.org/10.1038/nbt...

  3. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    Published
    Sept. 9, 2016, 2:43 p.m.
    ...perturbations – in the case of screens using CRISPR technology, gRNAs to knockout genes – are essential screening...the validity of your biological model and the technical performance of your assay. But, in many discovery-oriented... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...

  4. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...are also the foundation of multiple important technologies, including FRET and optogenetics. Even though...photoconvert GFP from green to RED! This simple technique has been shown to work in plant, Drosophila and...protonated form of GFP is photoconverted, so this technique may not be appropriate for high-pH cellular compartments...

  5. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...career goals, and celebrate accomplishments. These techniques are laid out in Vincent et al., and are easily...a recap of accomplishments, from learning new techniques to finally figuring out a tough experiment, sets... in every lab, but the principles behind this technique are beneficial for researchers at all stages. ...

  6. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...inner beauty of familiar flowers." This new x-ray technique provides scientist with a new method to explore...by Darwin on his voyage. Previous destructive techniques were incompatible with the need to conserve these...Richards’ work is held by The Museum of Jurassic Technology in LA, where it is currently on display.   Many...

  7. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...service. A small subset of AAV plasmids will have technical or legal restrictions that make them ineligible...be reviewed by Addgene for legal approvals and technical compatibility with our production process. This...found in our in-stock preps. If a request has technical considerations that may affect expected titers...

  9. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...to learn techniques from each other, access specialized equipment, or ensure that techniques are being...part of your collaboration. Luckily, there are techniques and tools that helped make my experience run ...

  10. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through...pathways.  This dissociation can be measured using a technique known as Bioluminescence Resonance Energy Transfer...effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists and ...

  11. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...the less acknowledged perks of scientific and technical training is that these educational paths prepare...encompasses millions of choices including pharma, tech transfer, management consulting, science communication...Small Company Big (e.g. Pharma) Small (e.g. Biotech) Develop expertise areas quickly Jack/Jane of...

  12. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-based toehold switches described.... These toehold switches can be used to quickly detect the presence of Zika virus. Read this post from...Combinatorial Genetic Screening CombiGEM is a technology that can be used to quickly barcode a set of ...

  13. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...more standardized methods for evaluating CRISPR technologies, including the ever important on/off-target ... are aimed at improving translation of CRISPR technology, but they would also bring benefits to researchers...pre-clinical research. However, standard terms and techniques are not enough to move the CRISPR field forward...

  14. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies Resources at Addgene.org...Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...

  15. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...excited about our new partnership with Allele Biotechnology which allows researchers to deposit plasmids...mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes...colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University...

  16. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070...vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585...4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019...

  17. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...with people, nurturing networks, as much as with technical knowledge. I leverage a breadth of knowledge to...I am constantly teaching – often explaining a technical piece of research to others outside that specialty... thinking about, for example, how to use new technologies to reach the right audiences and how open access...

  18. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...present in prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized...the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub 2016 May 2. PubMed PMID: 27136078. Find...Resources on Addgene.org Check Out Other Argonaute Technologies from the Doudna Lab Browse the CRISPR Collection...

  19. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...many developments in science and the plasmids, technologies, and researchers behind them. As part of our...started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed to provide...will be sure to keep our eyes open for any new technologies that will bring about the next revolution in...

  20. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...individuals in their 20’s who’ve built multibillion dollar tech companies using only laptops and some server space...our network.  We started with the idea of a biotechnology investment fund that would apply a percentage... biotechnology...
Showing: 281 - 300 of 693 results