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We narrowed to 703 results for: Tec

Showing: 281 - 300 of 703 results
  1. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...using the streak plate technique. Time for the #BioSci3319 #StreakPlateChallenge part 1. Lab B needs a...Sciences at Missouri University of Science and Technology, has been teaching the microbiology lab every...
  2. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...CRISPR continues to grow as the genome editing technique has developed and improved. The CRISPR posts written... for more detailed information about this new technology. Our Plasmids 101 series continues to grab scientists...questions or if you have a topic, researcher, or technology you would like us to feature in the coming year...
  3. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...got to learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale...DeNatale and programming with our Chief Architect Pam McA' Nulty (check out our blog post on CRISPR software...presentation on the emerging CRISPR genome editing technology was a rousing success! Many of the students were...
  4. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ... in skeletal muscle by exercise and endurance, protects the brain from depression. Mice that are genetically...stressors as usual, but somehow their brains were protected. Effects of Activation of the PGC-1a1 Pathway ...levels of kynurenine in the body, the brain is protected from stress-induced biochemical changes that could...
  5. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening to microbes...vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression ... high spatial resolution are benefits of this technique. It is even possible to multiplex ultrasound dyes...
  6. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...blog post, keeping up with the newest CRISPR technologies and their applications can be exhausting. A ...addition to mutations in the spacer itself, will protect the HR template from the Cas9. Again, if it's within...of the Cong et. al (2013) Science paper). If you detect positive HR, isolate single-cell colonies, grow...editing, regulating and targeting genomes. Nature Biotechnology, 32(4), 347–355. https://doi.org/10.1038/nbt...
  7. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    Published
    Sept. 9, 2016, 2:43 p.m.
    ...perturbations – in the case of screens using CRISPR technology, gRNAs to knockout genes – are essential screening...the validity of your biological model and the technical performance of your assay. But, in many discovery-oriented... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...
  8. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...are also the foundation of multiple important technologies, including FRET and optogenetics. Even though...photoconvert GFP from green to RED! This simple technique has been shown to work in plant, Drosophila and...protonated form of GFP is photoconverted, so this technique may not be appropriate for high-pH cellular compartments...
  9. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...career goals, and celebrate accomplishments. These techniques are laid out in Vincent et al., and are easily...a recap of accomplishments, from learning new techniques to finally figuring out a tough experiment, sets... in every lab, but the principles behind this technique are beneficial for researchers at all stages. ...
  10. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...inner beauty of familiar flowers." This new x-ray technique provides scientist with a new method to explore...by Darwin on his voyage. Previous destructive techniques were incompatible with the need to conserve these...Richards’ work is held by The Museum of Jurassic Technology in LA, where it is currently on display.   Many...
  11. Addgene’s Expanding Collection of Research Tools for Industry Scientists

    Type
    Blog Post
    Published
    Jan. 13, 2026, 2:15 p.m.
    ...editor deposited by Profluent Bio, maintains Cas9 architecture, but is almost two hundred mutations away from...expression analysis and tools for tracking and detecting proteins fused to NanoLuc, HaloTag, SmBiT, and...date information. If you are a depositing lab or Technology Transfer Office interested in expanding the availability...
  12. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...service. A small subset of AAV plasmids will have technical or legal restrictions that make them ineligible...be reviewed by Addgene for legal approvals and technical compatibility with our production process. This...found in our in-stock preps. If a request has technical considerations that may affect expected titers...
  13. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through...pathways.  This dissociation can be measured using a technique known as Bioluminescence Resonance Energy Transfer...effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists and ...
  14. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...to learn techniques from each other, access specialized equipment, or ensure that techniques are being...part of your collaboration. Luckily, there are techniques and tools that helped make my experience run ...
  15. Strengthening Foundations: Reflections on 2025 and What's Ahead for 2026

    Type
    Blog Post
    Published
    Feb. 5, 2026, 4:15 p.m.
    ...collection to uncover how key plant defense genes protect crops from devastating disease. The collective ...accessible to scientific entrepreneurs. From emerging technologies to underrepresented research areas, we're working..., M. (2025). Comparison of immunogenicity and protection efficacy of self-amplifying and circular mRNA...
  16. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...excited about our new partnership with Allele Biotechnology which allows researchers to deposit plasmids...mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes...colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University...
  17. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...more standardized methods for evaluating CRISPR technologies, including the ever important on/off-target ... are aimed at improving translation of CRISPR technology, but they would also bring benefits to researchers...pre-clinical research. However, standard terms and techniques are not enough to move the CRISPR field forward...
  18. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies Resources at Addgene.org...Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...
  19. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...with people, nurturing networks, as much as with technical knowledge. I leverage a breadth of knowledge to...I am constantly teaching – often explaining a technical piece of research to others outside that specialty... thinking about, for example, how to use new technologies to reach the right audiences and how open access...
Showing: 281 - 300 of 703 results