We narrowed to 644 results for: Vars

...size_unit":"px","font":"helvetica","fallback":null,"variant":null,"size":18},"button_color":{"color":"#0052a9...to the intended edit rather than some passenger variant that came along with the single cell clone (although...SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options that can...

...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...spent my senior year isolating pheromones from various C. elegans mutants by myself, I would have slowly...team should have a leader, other team roles will vary depending on the type of team. Knowing what roles...

...integrations per cell, and my stable cell pool had wildly variable Cas9 expression (Figure 1). This is a problem ...monoclonal lines (Figure 2) and I was surprised at how variable Cas9-expression was across the different cells...expressing A549 cells transduced with BRAF gRNA at various MOIs. The presence of the 428 bp Surveyor nuclease...

...work. Alina Chan is a postdoctoral researcher at Harvard Medical School working on human artificial chromosomes...Science in Albany, NY, many tables were set up with various science demonstrations, and students could walk...engineering synthetic microbial communities at Harvard University to studying an evolutionary arms race...

...delivered and expressed in multiple somatic cells via various methods. Some of the more common delivery methods...cells [31] Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...training from Massachusetts General Hospital and Harvard Medical School. Connect with her on LinkedIn @ ...

...lines, some of which are commercially available. Various proteomic and transcriptomic datasets can be used... confirm. As such, the approach used may need to vary according to the target, sample type, and available... J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon, V., Eckmann, P., Worrall, ...

...Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications (such as visualize...

...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...

...textbooks. I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the ...foundations of synthetic biology, DNA assembly, and various applications. I think these papers are good starting...

... feature step-by-step instructions to navigate various lab processes.  Our most recent protocol video ...feature interviews with real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their...

...for our audience of biological scientists from a variety of different fields. Exposure - With over 100,000... Network - Addgene will promote your blog on a variety of social media outlets meaning you’ll have the...

...a small start-up is very interesting. It’s very varied. There’s IP work to do, general corporate work,...is bringing experience and judgement to a wide variety of business decisions that are not “legal” at all...

...spend some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use his ...Seeding Labs Plasmid grant and is working on a variety of projects in Ghana.     Special thanks to our...

...’s molecular biology work has focused on using various cloning methods and tools to construct chimeric...like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins. Dr. Duedu’s plans for these...

...throughput washing devices that are compatible with a variety of pipette tips. This is part of a cultural change...hazardous. Then, they use this material to produce various grades of plastic lumber and distribute it to industrial...

...found. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...rush to give honest feedback to the manager for a variety of reasons. They may feel that giving feedback ...

...Caulobacter cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. ... expression of dCas9 and gRNAs whose expression varies depending on transgene design and delivery. They...

...prominence among all the noise. Scientists have a variety of metrics they can use to evaluate the impact ... this feature will draw attention to the great variety of plasmids in the repository. And last, but certainly...

..., and to understanding how nuclear organization varies across the life of a cell, or how organization ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...

...drawbacks. First, the efficiency of Cre recombination varies for different constructs and cell types. Second...shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical neurons in layer...