We narrowed to 643 results for: Vars
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  TypeBlog PostPublishedNov. 28, 2017, 2 p.m....researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...spent my senior year isolating pheromones from various C. elegans mutants by myself, I would have slowly...team should have a leader, other team roles will vary depending on the type of team. Knowing what roles...
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  With an Eye Towards the Future, We Look Back at the March for ScienceTypeBlog PostPublishedOct. 24, 2017, 1:54 p.m....work. Alina Chan is a postdoctoral researcher at Harvard Medical School working on human artificial chromosomes...Science in Albany, NY, many tables were set up with various science demonstrations, and students could walk...engineering synthetic microbial communities at Harvard University to studying an evolutionary arms race...
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  Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and TransdifferentiationTypeBlog PostPublishedNov. 6, 2018, 1:12 p.m....delivered and expressed in multiple somatic cells via various methods. Some of the more common delivery methods...cells [31] Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...training from Massachusetts General Hospital and Harvard Medical School. Connect with her on LinkedIn @ ...
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  Antibody Validation for Flow CytometryTypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....lines, some of which are commercially available. Various proteomic and transcriptomic datasets can be used... confirm. As such, the approach used may need to vary according to the target, sample type, and available... J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon, V., Eckmann, P., Worrall, ...
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  The 12 Days of CRISPRTypeBlog PostPublishedJan. 3, 2023, 2:15 p.m....CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...
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  It’s CRISPR Week at Addgene!TypeBlog PostPublishedMay 13, 2019, 12:01 p.m....Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications (such as visualize...
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  The Codon Guide to Synthetic BiologyTypeBlog PostPublishedSept. 14, 2023, 1:15 p.m....textbooks. I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the ...foundations of synthetic biology, DNA assembly, and various applications. I think these papers are good starting...
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  New Educational Resource: The Addgene Videos PageTypeBlog PostPublishedJune 16, 2017, 2 p.m.... feature step-by-step instructions to navigate various lab processes. Our most recent protocol video ...feature interviews with real Addgenies who have a variety of roles at Addgene. You’ll hear all about their...
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  Call for Guest BloggersTypeBlog PostPublishedMarch 2, 2016, 3 p.m....for our audience of biological scientists from a variety of different fields. Exposure - With over 100,000... Network - Addgene will promote your blog on a variety of social media outlets meaning you’ll have the...
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  Kwabena Duedu on Public Health, Biofuels, and Doing Science in GhanaTypeBlog PostPublishedDec. 20, 2016, 3:30 p.m....spend some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use his ...Seeding Labs Plasmid grant and is working on a variety of projects in Ghana. Special thanks to our...
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  Interview with Bart Newland, Addgene’s Newest Board MemberTypeBlog PostPublishedAug. 3, 2021, 1:15 p.m....a small start-up is very interesting. It’s very varied. There’s IP work to do, general corporate work,...is bringing experience and judgement to a wide variety of business decisions that are not “legal” at all...
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  Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding LabsTypeBlog PostPublishedJune 15, 2016, 1:30 p.m....’s molecular biology work has focused on using various cloning methods and tools to construct chimeric...like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins. Dr. Duedu’s plans for these...
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  Bringing Sustainable Practices to the Lab: InnovationsTypeBlog PostPublishedMarch 7, 2019, 2:39 p.m....throughput washing devices that are compatible with a variety of pipette tips. This is part of a cultural change...hazardous. Then, they use this material to produce various grades of plastic lumber and distribute it to industrial...
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  Management for Scientists: Seeking FeedbackTypeBlog PostPublishedJan. 8, 2015, 2:34 p.m....found. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...rush to give honest feedback to the manager for a variety of reasons. They may feel that giving feedback ...
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  What's New in CRISPR - March 2020TypeBlog PostPublishedMarch 10, 2020, 1:15 p.m....Caulobacter cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. ... expression of dCas9 and gRNAs whose expression varies depending on transgene design and delivery. They...
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  Increasing Your Work's Visibility with Addgene: Citation, Search, and CollectionsTypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....prominence among all the noise. Scientists have a variety of metrics they can use to evaluate the impact ... this feature will draw attention to the great variety of plasmids in the repository. And last, but certainly...
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  Mapping the 4D nucleome with CRISPR/Cas9TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m...., and to understanding how nuclear organization varies across the life of a cell, or how organization ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...
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  Cre-ating New Methods for Site-specific Recombination in DrosophilaTypeBlog PostPublishedMay 12, 2015, 1:32 p.m....drawbacks. First, the efficiency of Cre recombination varies for different constructs and cell types. Second...shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical neurons in layer...
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  Fluorescent Proteins 101: Fluorescent BiosensorsTypeBlog PostPublishedMay 18, 2017, 2:30 p.m....biosensors, but keep in mind that there is a lot of variety in how biosensors work, and you should always check...process (e.g. enzyme activity) over time or under various conditions Localization within cells Fluorescence...
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  SunTag and Fluorescent ImagingTypeBlog PostPublishedMarch 28, 2017, 2:30 p.m.... localization and expression may change across various conditions. However, this system is far from perfect...you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so...