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We narrowed to 642 results for: Vars;

Showing: 281 - 300 of 642 results

  1. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...vector for this step may save time as it reduces variability that may be encountered with DIO/FLEx vectors...off-target expression. Since recombination rates vary between every lot of AAV vector, a range of dilutions...rare for a methods section to include all of the variables that need to be accounted for (i.e. serotype, ...

  2. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...work. Alina Chan is a postdoctoral researcher at Harvard Medical School working on human artificial chromosomes...Science in Albany, NY, many tables were set up with various science demonstrations, and students could walk...engineering synthetic microbial communities at Harvard University to studying an evolutionary arms race...

  4. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...lines, some of which are commercially available. Various proteomic and transcriptomic datasets can be used... confirm. As such, the approach used may need to vary according to the target, sample type, and available... J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon, V., Eckmann, P., Worrall, ...

  5. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications (such as visualize...

  6. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...

  7. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...textbooks. I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the ...foundations of synthetic biology, DNA assembly, and various applications. I think these papers are good starting...

  8. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ... feature step-by-step instructions to navigate various lab processes.  Our most recent protocol video ...feature interviews with real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their...

  9. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...for our audience of biological scientists from a variety of different fields. Exposure - With over 100,000... Network - Addgene will promote your blog on a variety of social media outlets meaning you’ll have the...

  11. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...a small start-up is very interesting. It’s very varied. There’s IP work to do, general corporate work,...is bringing experience and judgement to a wide variety of business decisions that are not “legal” at all...

  13. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...throughput washing devices that are compatible with a variety of pipette tips. This is part of a cultural change...hazardous. Then, they use this material to produce various grades of plastic lumber and distribute it to industrial...

  14. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...found. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...rush to give honest feedback to the manager for a variety of reasons. They may feel that giving feedback ...

  15. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...Caulobacter cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. ... expression of dCas9 and gRNAs whose expression varies depending on transgene design and delivery. They...

  17. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ..., and to understanding how nuclear organization varies across the life of a cell, or how organization ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...

  18. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...drawbacks. First, the efficiency of Cre recombination varies for different constructs and cell types. Second...shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical neurons in layer...

  19. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors, but keep in mind that there is a lot of variety in how biosensors work, and you should always check...process (e.g. enzyme activity) over time or under various conditions Localization within cells Fluorescence...

  20. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ... localization and expression may change across various conditions. However, this system is far from perfect...you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so...
Showing: 281 - 300 of 642 results