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  1. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...textbooks. I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the ...foundations of synthetic biology, DNA assembly, and various applications. I think these papers are good starting...

  2. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ... feature step-by-step instructions to navigate various lab processes.  Our most recent protocol video ...feature interviews with real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their...

  3. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...for our audience of biological scientists from a variety of different fields. Exposure - With over 100,000... Network - Addgene will promote your blog on a variety of social media outlets meaning you’ll have the...

  5. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...a small start-up is very interesting. It’s very varied. There’s IP work to do, general corporate work,...is bringing experience and judgement to a wide variety of business decisions that are not “legal” at all...

  7. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...throughput washing devices that are compatible with a variety of pipette tips. This is part of a cultural change...hazardous. Then, they use this material to produce various grades of plastic lumber and distribute it to industrial...

  8. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...found. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...rush to give honest feedback to the manager for a variety of reasons. They may feel that giving feedback ...

  9. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...Caulobacter cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. ... expression of dCas9 and gRNAs whose expression varies depending on transgene design and delivery. They...

  11. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ..., and to understanding how nuclear organization varies across the life of a cell, or how organization ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...

  12. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...drawbacks. First, the efficiency of Cre recombination varies for different constructs and cell types. Second...shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical neurons in layer...

  13. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors, but keep in mind that there is a lot of variety in how biosensors work, and you should always check...process (e.g. enzyme activity) over time or under various conditions Localization within cells Fluorescence...

  14. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ... localization and expression may change across various conditions. However, this system is far from perfect...you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so...

  15. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...great overview of the development of CRISPR into a varied research toolbox, as well as additional resources...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and...

  16. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and reduced... Various Addgenies...

  18. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell types. Because the PhiC31 recombinase...

  19. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...into a vector, scientists may utilize one of a variety of cloning methods (restriction enzyme, ligation...research or for therapeutics Addgene has compiled various educational resources to facilitate plasmid use...
Showing: 281 - 300 of 633 results