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We narrowed to 340 results for: abo.4

Showing: 281 - 300 of 340 results

  1. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...the National Grants Management Association (NGMA). 4. Develop a professional development strategy  Build...on becoming grant writers. When we asked others about how they became grant professionals, mid- to late-career...late-career grant professionals have similar stories about a time when their passion for an organization or... logic models, established partnerships and collaborations, and commitments to and proof of compliance...grants. It is no surprise that the U.S. Bureau of Labor and Statistics shows that employment of grant writers... skills & passion  Skills such as research, collaboration across multiple disciplines, writing, project...development strategy builds on the steps listed above and formalizes your development. You may consider...

  2. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ... and worked as a technical support specialist for 4 years. Read on for insights from my time in technical...disadvantages of this overall rewarding role. Learn about Other Addgenies and Their Careers in Our Career ...general experimental guidance, and resolve complaints about products. As you might expect, different companies...order to enhance the OD signals and then complain about high background. In many instances, variations ...protocol. And customers may tell all sorts of details about the application, just not the ones that matter. ...communication, and is particularly excited to hear about novel developments in the fields of gene and cellular...Academic v. Non-Academic Career Decisions Learn about Science Career Options Explore Tips for Technical...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Cancer 2.4 (2002): 315-319. PubMed PMID: 12001993. 4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover...Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about the Dangers...Misidentified or cross-contaminated cells have been known about since 1966 when Stanley Gartler reported that most...there is a loss of reputation that can impede collaborations, especially with industry. Today we also have...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...relevant to avian magnetoreception. Nature Chemistry, 8(4), 384–391. https://doi.org/10.1038/nchem.2447.  Lee...our tissues unimpeded. Andrew and Maria dreamed about the possibilities of a magnetoresponsive fluorescent...of improved response. Then, just when Andrew was about to suggest she deprioritize the project, Maria’s...with a strong commitment to exploration and collaboration. Andrew is a physicist with a background in ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...interested in working on these questions or collaborating, the York Lab is eager to hear from you. Find...

  5. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...mediated knock-ins in mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016/j.jbiotec.2019.11.010 Wright...CRISPR Featured Topic Page Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to ... post on increasing HDR efficiency to read more about optimizing this distance. Homology arms and types...screening strategies for your experiment. Also learn about Addgene’s knock-in plasmids for reagents to help...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...the same sample, you could end up with a titer of 4 x 1012 genome copies/mL or 8 x 1012 genome copies/... droplet generator, you will have to be careful about transferring your droplets from the droplet generator...Additional resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts ...collection of viral vector related protocols Learn more about the Addgene viral service ...

  7. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... PMID 25417800. PubMed Central: PMCID PMC4282009. 4. Yosef, Ido, Miriam Manor, Ruth Kiro, and Udi Qimron...organizations are working to help educate the public about proper antibiotic usage. Although these steps are...laudable, they likely won’t solve the growing problem. Above all, we need new antimicrobials, ideally ones for...used to deliver any of the three systems discussed above, with the caveat that phages are very diverse, and...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...dissolved in DMSO which allows both biotin and biotin-4-fluorescein to be dissolved at a higher concentration... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's... a cell. The Goedhart lab, Gadella lab, and collaborators have an ongoing effort to improve genetically..., that are optimized for excitation wavelengths above 1,000 nm and enable two-color imaging with red fluorescence...

  9. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...beads are collected by centrifugation or a magnet. 4) Unbound proteins are washed away. 5) The target protein...proteins or nucleic acids. Read on to learn more about this versatile technique.  Immunoprecipitation ...proteins in an IP are not denatured (learn more about antibodies here!) When using Protein A/G to immobilize...eluted by boiling the beads in SDS. (To learn more about Western blotting check out our blog post The Basics...

  10. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...endotoxin-free plasmid purification kits.     Figure 4: Schematic of the barcode matching system and PCR-... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...generated from lentiviral preparations (described above) that are subject to a concentration step prior ...

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...Nature Genetics 28, 92-95. PubMed PMID: 11326284. 4. Bennett J, 2017. Taking Stock of Retinal Gene Therapy...famously visited Congress to help increase awareness about the potential of gene therapy!). It would take another...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...

  12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...PMID: 18355003. PubMed Central PMCID: PMC2802578. 4. Xue, Lin, et al. "Imaging and manipulating proteins... the frontiers of biological imaging.  *Worried about your budget? Email [email protected] Resources on the Addgene Blog Learn About Other Protein Tags Catch Up on Your Fluorescent ...

  13. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... aversion/anxiety. The mouse can move freely on a 4-arm cross and choose to stay on an open arm (risky...distant regions. They also don’t give any clues about the types of neurons involved or whether their involvement... Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...PMC5526198. Find plasmids from this paper at Addgene. 4. East-Seletsky, Alexandra, et al. "RNA Targeting by...potential applications of Cas13a given what we know about its structure and function? For starters, Cas13a... Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 ...

  15. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...276.32 (2001): 29621-29624. PubMed PMID: 11408473. 4. Subach, Fedor V., et al. "Monomeric fluorescent timers...Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch Up On Your...

  16. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...Mauch,  K. Deininger, et al. (2007). Nature Methods, 4, 331–336. Pubmed. P. Keller, M.B. Ahrens.  (2015) ...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...histories, accurate to within 40 minutes. Figure 4: Time-resolved protein ticker tape. A) Design of iPAK4...section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts, including...

  18. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...transcriptional activation screening. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 ...ordering a pre-made library, you don’t need to worry about amplifying and producing lentivirus. But pre-made...

  19. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...just need to figure out how to make it suspenseful. 4. Start preparing your talk early - like today Don’...interesting content Talk about something you know a lot about and that you care about. Don’t try to present...engaging and memorable. If you read my story above about the presentation faux pas, you are more likely...the room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs ...dynamite presentation?  To that end, let’s chat about giving science talks and how to make them serve ...other people give great talks We are not talking about lab meeting here. Lifeless presentation of experimental...You want to give the audience something to think about, argue with, or learn from. Some of the best talks...

  20. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...general mission to share- as opposed to sell- science. 4. You can prepare answers beforehand. Joanne answered...not too worried about selling yourself and really do just want to get information about yourself and your...where users can participate in forum discussions about a wide variety of topics. Individual forums are ...where users can ask designated scientists anything about a particular topic. Given Joanne’s expertise in... is well moderated. One of our original worries about sharing science advice on Reddit was that we’d be...answering questions. r/Science has pretty strict rules about not being too sales-y in posts which falls in line...any scientists to refer to if they have questions about science careers, women in STEM, work-life negotiation...
Showing: 281 - 300 of 340 results