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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 806 results for: cha;

Showing: 281 - 300 of 806 results

  1. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...methods measure different characteristics of the solution. These characteristics give us information on ...temperature parameters. For example, we found that changing the annealing temperature of the PCR from 60° ...al. "Systemic errors in quantitative polymerase chain reaction titration of self-complementary adeno-associated...PMCID: PMC3216358. 4. Lock, Martin, et al. "Characterization of a recombinant adeno-associated virus type...

  2. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...better prepare for your career, and increase your chances of landing that next coveted job! Professional...development (or lack thereof) One of the biggest challenges facing students today is that many graduate and...your credentials to HR, greatly increasing your chances of getting a phone interview! If you don’t have...touch and reach back out when you are looking to change positions in the future. Anyone looking for work... Michael G. Lemieux...

  3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...inverted vectors expressing light inducible ion channels (plasmid # 20298 for example) have revolutionized...FLEx vectors expressing the light activatable ion channel under the control of Cre recombinase are used in... M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range...Sadowski. 1985. “The FLP Recombinase of the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently...PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination...

  4. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...Monster probes at Addgene Uses of smFPs 1. Multi-channel connectomic tracers Fluorescent proteins such ...anti-FP antibodies. GFP emits light in only a single channel. In multi-color imaging experiments, the second...making them difficult to use in a multicolor/multichannel imaging set-up. In contrast, smFP tags can be...University Press, New York. pp 719. 3.Rizzo, Mark A., Michael W. Davidson, and David W. Piston. "Fluorescent ...4. Shaner, Nathan C., George H. Patterson, and Michael W. Davidson. "Advances in fluorescent protein technology...

  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...Enzymatic Domains Guide RNA Target PAM (PFS) Cleavage Mechanism Cas9 HNH, RuvC TracrRNA, crRNA DNA 5' NGG... Leptotrichia shahii (LshCas13a) to begin to characterize this new effector (Abudayyeh et al., 2016). ...and cleavage through a series of conformational changes in the Cas13a molecule. Like Cas9, Cas13a tolerates... plant cells (Abudayyeh et al., 2017). They characterized Cas13a from Leptotrichia wadei (LwaCas13a), ..., Sergey, et al. "Discovery and functional characterization of diverse class 2 CRISPR-Cas systems." Molecular...

  6. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...and Discovery As part of their quest to find, characterize, and develop new CRISPR systems and technologies...proteins that could cut RNA or DNA via a CRISPR mechanism. The weird black pellet's DNA yielded a new CRISPR...Prime editing — the ability to now make any small changes, including base edits, insertions, and deletions...right integrase for this new approach proved a challenge. The old standby of CRE/flp didn’t work – it turns...be independent of gene repair.) This lowers the chance of the insert being “dropped” in the wrong place...

  7. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...gRNA together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb...the group to focus their efforts on SaCas9 characterization for in vivo studies. One of the pitfalls of...genes, they saw indel formation and phenotypic changes as early as 1 week post-injection. Livers from ...jejuni (CjCas9) is the smallest Cas9 ortholog characterized to date. Kim et al. successfully used CjCas9...transgenes might seem daunting, it’s actually a challenge that the field has overcome with split AAVs. In...

  8. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...When you think of Addgene, chances are software development is not one of the things that comes to mind...the flexibility to adapt and easily respond to changing needs of the teams at Addgene. Another advantage...to bake a cake. Instead, you go to a store and purchase flour. However, buying a bag of flour is not the...updated in several years) or they make significant changes that make it incompatible with our software. When...Python 2.7 to Python 3.5. There are significant changes to the language and it took us a total of two years...

  9. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...to interfere with the function of resident ER chaperones and may impact cellular function or possibly ...For example, the formation of inappropriate inter-chain disulfide bonds between FPs blocks Golgi complex-localized...protein activity. We encourage investigators to characterize FP-fusion proteins functionally relative to ...moxFPs. The inherent brightness and spectral characteristics of the moxFPs are comparable to the non-optimized...postdoctoral fellow at the University of North Carolina at Chapel Hill. Under the co-mentorship Blossom Damania and...

  10. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...centered within GFP/RFP bicistronic reporters characterized in Cambray et al., 2013. These terminators can...: 2388-2393. Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification...metalloproteinase: López-Pelegrín, Mar, et al. "A novel mechanism of latency in matrix metalloproteinases." Journal...size from 4 to 156 kDa, enabling the user to characterise the efficiency of their cell-free system in ...5373-5383. Walkiewicz, Katarzyna, et al. "Small changes in enzyme function can lead to surprisingly large...

  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...sensor relies on a change in localization to the cell membrane and not a direct change in the fluorescent...better response kinetics and photochemical characteristics than previous NIR calcium reporters iGECI and...   Matlashov ME, et al. Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium... B) LiLac responses are highly resistant to pH changes. Image adapted from Koveal et al. 2022, under CC-BY...

  12. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...found it easy to drop into a chair and chat with me remotely via an audio chat (Fig. 4).  Learn: This works...small operation, so sometimes it can be a big challenge. But ask any filmmaker or videographer and they...you can usually see a director sitting in their chair not watching their actors live on set, but peering...

  13. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2024, 1:15 p.m.
    ...designed to be a helpful desktop reference. Each chapter brings together all of our 101 blog posts on an...to antibodies as research tools, you may find “Chapter 1: What is an Antibody” quite useful. If you’re... design your first flow cytometry experiment, “Chapter 7: Sorting” is there to help guide you through ...find an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody for Your Experiment...

  14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ... material, and variations between serotypes can change how the viral particle sneaks its way into cells...Dhiab, J., Cordova, G., Arandel, L., Marhic, A., Chapart, M., Vasseur, S., Benkhelifa-Ziyyat, S., Bigot,...Y., Jae, L. T., Wosen, J. E., Nagamine, C. M., Chapman, M. S., & Carette, J. E. (2016). An essential receptor...Gaj, T., Lavzin, M., Ritola, K. D., Lindo, S., Michael, S., Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen... Dudman, J. T., Hantman, A. W., Looger, L. L., Schaffer, D. V., & Karpova, A. Y. (2016). A Designer AAV...

  15. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...positively charged groups, such as aldehydes in fixed samples. To address this, block positively charged groups...the label control for all new samples, protocol changes, or when high background labeling occurs (Burry...critical for data interpretation, especially when changes in protein expression are the experimental readout...       References and resources References Burry, Richard W. “Controls for Immunocytochemistry.” Journal ...

  16. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...promoter, and titer.     Figure 1: Flow chart depicting a) steps for selecting serotypes and promoters... different receptors, co-receptors, and other mechanisms to gain entry into the cell (Dudek et al., 2018... different brain regions.  Image modified from Aschauer et al., 2013, Figure 1. Used under Creative Commons...the region you want to cover - and as your titer changes, so may your needed volume (de Backer et al., 2010....2014.00076 References and Resources References Aschauer, D. F., Kreuz, S., & Rumpel, S. (2013). Analysis...

  17. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method that harnesses the...First, you’d construct a plasmid with your desired changes, long homology arms and a selection marker, then...template. Although effective, ivTRT may be more challenging for sequences with complex secondary structure...-purified DNA template.   Many thanks to Channabasavaiah B. Gurumurthy, Masato Ohtsuka, and Rolen M....PMC5434640 Miura, Hiromi, Rolen M. Quadros, Channabasavaiah B Gurumurthy, and Masato Ohtsuka. “Easi-CRISPR...

  18. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...added, it binds tTA, resulting in a confirmation change that prevents binding to the promoter and turning...added, it binds cTA, resulting in a confirmation change that prevents binding to the promoter and turning...this way, you can interrogate the activity of uncharacterized promoters. Similarly, placing UAS upstream ...1472-6750-6-43Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Cumate-inducible gene expression system for ...genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197...

  19. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...had record readership with over 75,000 views. Changing Labor Laws Bring Increased Postdoc Wages Our most...information for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,” Future...for some of these institutions to manage these changes quickly and harmlessly. Learn much more about the...Reasons to Use Reddit for Science Communication Changing Labor Laws Bring Increased Postdoc Wages I hope...

  20. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    Published
    April 5, 2016, 2:30 p.m.
    ...to face global challenges, we hope to empower those that need to face these challenges locally. We look...themselves on the front lines of emerging global challenges. However, these same researchers face significant...international distribution As you can see from the chart below, we are making great progress toward spreading...
Showing: 281 - 300 of 806 results