We narrowed to 515 results for: gan
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TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...
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Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. CRISPR... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....techniques are also being applied to study model organisms such as Drosophila and zebrafish. Brainbow in ...important insights into the complicated physical organization of the brain and other biological systems. Interested... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m...., B. K., Aoi, Y., Iwanaszko, M., Howard, B. C., Morgan, M. A., Andersen, G. D., Bartom, E. T., & Shilatifard...Efficient proximity labeling in living cells and organisms with TurboID. Nature Biotechnology, 36(9), 880... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m....which allow AAVs to broadly spread throughout an organism, including difficult-to-target tissues like the...Byrne, L. C., Klimczak, R. R., Visel, M., Yin, L., Merigan, W. H., Flannery, J. G., & Schaffer, D. V. (2013... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly... -
Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m....Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....post was contributed by guest bloggers and IMP organizers Rosario F. Godino, Rocío López-Diego, & Zafira... Students also need more access to reputable, organized networks connecting international experts and ... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....skilled at presenting and answering questions. Organizing a thoughtful engaging talk takes significant ...successful writers about their strategies for organizing and self-critiquing their own writings. 8. Learn... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....expensive, and not to mention frustrating. One elegant solution to sidestep the antibody problem is to...References 1. Bressan RB, Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant ... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....classification While CRISPR systems now seem neatly organized into their respective categories, arriving at ...similar levels. It was only recently that researchers began building optimal plasmids to harness the power of... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture is preserved, ...from the bloodstream. Then, via a blood draw or organ harvest, the labeled cells can be analyzed using... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m....transdifferentiation factors are directly injected into the organ of interest for in vivo transdifferentiation. Multiple... Williams, L.A., B.N. Davis-Dusenbery, and K.C. Eggan, SnapShot: directed differentiation of pluripotent... -
CRISPR/Cas9 FAQs Answered!
TypeBlog PostPublishedMarch 13, 2014, 4:08 p.m....with Addgene Senior Scientist, Matt Ferenc, to organize the CRISPR forum's most popular questions and ...https://doi.org/10.1038/nbt.2842 Soldner, F., Laganière, J., Cheng, A. W., Hockemeyer, D., Gao, Q., Alagappan... -
28 Hot Plasmid Technologies from 2015
TypeBlog PostPublishedDec. 23, 2015, 3:30 p.m....acquired from the the fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which...including subcellular visualization of 6 different organelles, a cell cycle indicator for mouse embryonic stem...monitoring cargo trafficking, and for discovering organelle contact sites. This suite of deposited fluorescent... -
Plasmids 101 eBook 4th Edition
TypeBlog PostPublishedSept. 5, 2023, 1:15 p.m....Dimers and Multimers" post. It's also been re-organized and updated with a fresh new look. We think this... -
Addgene’s First AAV Data Hub Challenge
TypeBlog PostPublishedSept. 20, 2022, 1:15 p.m....even win a $500 travel prize in our open data extravaganza! Anyone can submit data from their CLOVER... -
A Case Study of Addgene Bioinformatics
TypeBlog PostPublishedMay 25, 2023, 1:15 p.m.... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog PostPublishedJan. 11, 2024, 2:15 p.m....decision allows us to remain aligned to our organizational values. To preserve the knowledge, conversations... -
New FLIS Plasmid (April Fools 2022)
TypeBlog PostPublishedApril 1, 2022, 1:15 p.m....size have allowed scientists to use it as a model organism to interrogate many scientific questions. But ...