We narrowed to 363 results for: nsf

1. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ...IF-ICC.) This method was chosen because cells transfected with a plasmid to express the target antigen ...

2. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   Published

   Nov. 24, 2020, 2:15 p.m.

   ...explaining more of her role, which eventually led me to transfer to her team and begin my product management (PM...

3. Plasmids 101: Screening Strategies Used in Plasmid Cloning

   Type

   Blog Post

   Published

   Sept. 15, 2020, 1:15 p.m.

   ...information (more on this below). Plating the transformation on ampicillin plates containing X-gal allows...

4. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   Published

   May 19, 2020, 1:15 p.m.

   ...sequencing as a first pass QC. The lab team manually transforms, innoculates, and performs DNA minipreps for ...

5. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...Scientists: What Makes a Good Leader Anyways?  Transferable Skills Guide: Managing a Team  ...

6. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   Published

   March 21, 2023, 1:15 p.m.

   ...of cell types that are typically ‘difficult to transfect’, such as primary cells. They can also be directly...

7. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ... host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a plasmid...

8. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...activation via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling...

9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...note that due to the size of the backbone, transformations require electroporation. References & Further...

10. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...cells after the plasmids have been introduced by transfection or electroporation. All you do to create a protein...

11. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ... include both scrambled RNA motifs and cells transfected with BirA* protein alone (i.e. no RNA bait). ...

12. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...off-target effects, but this advantage likely won’t transfer to all genes we’d like to target. In addition,...

13. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...on the Addgene blog: Check out this guide on Transferable Skills: External Collaborations Learn about ...

14. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    Published

    Jan. 14, 2019, 2:08 p.m.

    ...lauded by another set of stakeholders - the tech transfer community. In a recent law review article we received...

15. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...targeting experiments allows you to visualize transfection efficiency and even track localization in cellular...

16. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...bonds, monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used...

17. What's New in CRISPR - November 2021

    Type

    Blog Post

    Published

    Nov. 18, 2021, 2:15 p.m.

    ...They further develop a version of SEND for gene transfer with pseudotyped viral particles, expanding options...

18. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...t have a system through which she could easily transfer her instructions for dealing with the samples ...

19. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ...production and storage, we grow a little extra and transfer 100 µl of glycerol stock to each of 2 96-well ...

20. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    Published

    Nov. 24, 2015, 3:30 p.m.

    ... in their 293 cell culture system. In cells cotransfected with GFP and the GBPs, GFP linked the two GBPs...