We narrowed to 324 results for: pho

1. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...using cloning rings in terms of growth rates, morphology or other physiological properties. An advantage...

2. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

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   July 13, 2017, 2:30 p.m.

   ...mother. Inside the pupal case, the larva will metamorphose into its adult form. In all, it takes roughly...

3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

4. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...change is usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether...

5. Degrading DNA with Cascade-Cas3

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   Blog Post

   Published

   Feb. 11, 2025, 2:15 p.m.

   ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...

6. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...

7. Fluorescent Tagging of Endogenous Genes with SapTrap

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   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

8. Genome-wide Screening Using CRISPR

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   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...

9. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

10. Great Results Start with Great Standard Curves

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    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...

11. Simplify Cloning with in vivo Assembly

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    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...

12. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

13. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

14. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...used in C. roseus, but processes specific to photosynthetic active tissues cannot be studied in hairy roots...

15. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...

16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ... regulated by guanosine [corrected] 3', 5'-bispyrophosphate: a model for programmed bacterial cell death...

17. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...provided moral support was invaluable.  Mixing metaphors and scientists - Culturing a scientific network...

18. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    Published

    Nov. 14, 2017, 2 p.m.

    ...HR, greatly increasing your chances of getting a phone interview! If you don’t have a particular employer...

19. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...support roles are primarily office based, and email/phone are the most popular ways to communicate with customers...

20. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator...