We narrowed to 317 results for: pho;

1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

2. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...change is usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether...

3. Genome-wide Screening Using CRISPR

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   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...

4. Simplify Cloning with in vivo Assembly

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   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...

5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

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   Sept. 6, 2018, 12:02 p.m.

   ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

7. Great Results Start with Great Standard Curves

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   Blog Post

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   Aug. 15, 2024, 1:15 p.m.

   ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...

8. Plasmids 101: Protein tags

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   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

9. Designing Your Chalk Talk for the Academic Job Interview

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   Blog Post

   Published

   March 12, 2019, 1:37 p.m.

   ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...

10. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ... regulated by guanosine [corrected] 3', 5'-bispyrophosphate: a model for programmed bacterial cell death...

11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...provided moral support was invaluable.  Mixing metaphors and scientists - Culturing a scientific network...

12. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...used in C. roseus, but processes specific to photosynthetic active tissues cannot be studied in hairy roots...

13. Hot Plasmids - November 2023

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    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ... promoter. For example, neurons treated with a phorbol ester activator gave fluorescent signal in the ...

14. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ... as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but ...

15. Hot Plasmids Spring 2024

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    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

16. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, ...

17. Transferable Skills Guide: Career Planning Resources

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    Blog Post

    Published

    Nov. 14, 2017, 2 p.m.

    ...HR, greatly increasing your chances of getting a phone interview! If you don’t have a particular employer...

18. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...support roles are primarily office based, and email/phone are the most popular ways to communicate with customers...

19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator...

20. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    Published

    Oct. 20, 2016, 2:30 p.m.

    ...notes which Evernote even allows me to access on my phone. I use it for everything from receipts to blogs ...