We narrowed to 321 results for: pho;

1. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...change is usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether...

2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ... of the highly conserved glutamine-fructose-6-phosphate aminotransferase (glmS) gene in the recipient ...

3. Degrading DNA with Cascade-Cas3

   Type

   Blog Post

   Published

   Feb. 11, 2025, 2:15 p.m.

   ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...

4. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...

5. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...form teratomas [11]. iPSCs also possess similar morphologies, growth properties, and express stem cell markers...

6. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...

7. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...

8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

9. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

10. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...

11. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator...

13. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...used in C. roseus, but processes specific to photosynthetic active tissues cannot be studied in hairy roots...

14. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...

15. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ... as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but ...

16. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

17. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, ...

18. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ... promoter. For example, neurons treated with a phorbol ester activator gave fluorescent signal in the ...

19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ...direction (which is not easy to see in colony PCR electrophoresis), is free of undesired mutations, and that ...

20. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...provided moral support was invaluable.  Mixing metaphors and scientists - Culturing a scientific network...