We narrowed to 325 results for: pho;

1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

2. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...change is usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether...

3. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...

4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ... of the highly conserved glutamine-fructose-6-phosphate aminotransferase (glmS) gene in the recipient ...

5. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...form teratomas [11]. iPSCs also possess similar morphologies, growth properties, and express stem cell markers...

6. Fluorescent Tagging of Endogenous Genes with SapTrap

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   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

7. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...’d like us to discuss. We are also available by phone or at [email protected] to answer your questions ...

8. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...using cloning rings in terms of growth rates, morphology or other physiological properties. An advantage...

9. Genome-wide Screening Using CRISPR

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   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...

10. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...

11. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

12. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...

13. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

14. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

15. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ... as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but ...

16. Hot Plasmids Spring 2024

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    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

17. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, ...

18. Hot Plasmids - November 2023

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    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ... promoter. For example, neurons treated with a phorbol ester activator gave fluorescent signal in the ...

19. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    Published

    Nov. 14, 2017, 2 p.m.

    ...HR, greatly increasing your chances of getting a phone interview! If you don’t have a particular employer...

20. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...support roles are primarily office based, and email/phone are the most popular ways to communicate with customers...