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Showing: 281 - 300 of 515 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...is displayed on the coats of bacteriophages, a process which links genotype and phenotype. Incubating ...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences 85:5879–5883...

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...frequency of each variant during the selection process. Each variant is assigned a functional score based...library frequency after selection. Key to this process is the ability to generate full libraries of mutant...

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...for biological spectroscopy and microscopy." Proceedings of the National Academy of Sciences 114.23 (2017... real-time multicolor luminescence imaging." Proceedings of the National Academy of Sciences (2015): 201418468...

  4. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...looking for, you are ready to begin the screening process. Want to set yourself up for a screening success...then a single, bulk validation may be enough to proceed with your experimental plan. Validating knockout...

  5. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ... mature tissues - no problem, as exon skipping proceeds through the non-homologous end joining (NHEJ) ...is upregulating HDR in mature tissues, as this process is necessary to precisely edit point mutations....

  6. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...value that helps you determine whether or not to proceed with a used or new item. To further illustrate ...we mentioned previously.  There is value in the process of creating a budget; you can share your budget...

  7. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...template. HDR, however, is a very low-efficiency process, and usually involves the need for single cell ...successful edits. This is a very time consuming process and should not be undertaken lightly! Indeed, truly...

  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms ...light-gated cation-selective membrane channel. Proceedings of the National Academy of Sciences, 100(24),...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...affect expression, before starting the selection process. Factors to think about are (1) your experimental...will allow you to efficiently move through this process.  Many other factors can also affect your AAV experiments...

  10. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but ...spatial analysis of cells in complex tissues. Proceedings of the National Academy of Sciences, 117(52),...

  11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...useful for the biotech industry, which is in the process of capitalizing on phage RBPs as diagnostic tools...DNA fragments with live host cells Now you can proceed with incubating this membrane with your cells of...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...not always guaranteed. To learn more about this process check out our Mouse Modeling blog posts: Part 1...have germline competent ESC lines. Second, the process is much quicker. It takes 3 months to generate ...

  13. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was constantly...the technology is so advanced that most of the procedures in the lab are done with robots. Young scientists...

  14. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...career and you'll become a better scientist in the process. 9. Avoid burnout The suggestions in this blog ...a diet rich in vegetables, foods with minimal processing, and low in empty calories will make you feel...

  15. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...incorporation of that sequence into the zygote’s genome, a process that’s highly unpredictable. Transposons, however...injection into the cytoplasm of a fertilized egg, a process which is inefficient when DNA is injected. Sleeping...

  16. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...medical practice, and understanding the scientific process can help us understand how new therapies can be...experience. Previous Addgene blogs have discussed the process that goes into choosing a good scientific mentor...

  17. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...your virus. Virus purification can be a tricky process. You have to centrifuge the culture supernatant...that favours virus precipitation. My tip for this process is to test several protocols that have been described...

  19. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...most important steps in the CRISPR experimental process is validating edits. Regardless of which CRISPR...not an edit in the target gene. The validation process, also known as CRISPR genotyping, is critical to...
Showing: 281 - 300 of 515 results