We narrowed to 308 results for: reporter

1. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional analysis of proteins...

2. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   Published

   June 28, 2016, 10:59 a.m.

   ...expressing Cas9 and single guided RNA." Scientific reports 3 (2013). PubMed PMID: 24284873. PubMed Central...

3. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol...

4. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   Published

   Nov. 25, 2015, 4 p.m.

   ...(the current median postdoc starting salary as reported by Paula Stephan is $40,000) Increase Knowledge...

5. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID: 25242321. Pubmed...

6. Harnessing Bacterial Toxins for Allelic Exchange

   Type

   Blog Post

   Published

   Aug. 15, 2019, 12:30 p.m.

   ...genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason...

7. Five Popular Model Organisms

   Type

   Blog Post

   Published

   April 11, 2019, 12:39 p.m.

   ...important gene co‑expression modules." Biomedical Reports 7.2 (2017): 153-158. PubMed PMID: 28804628. PubMed...

8. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...fluorescent proteins in budding yeast." Scientific reports 9.1 (2019): 2234. PubMed PMID: 30783202. PubMed...

9. March for Science

   Type

   Blog Post

   Published

   April 21, 2017, 2:30 p.m.

   ...Immigrants National Science Foundation: Universities Report Fourth Straight Year of Declining Federal R&...

10. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...already commercially available although they are reported to have high error rates (10). It is projected...

11. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...ten colors (see table to the right); in various reports, Sanes and Lichtman have observed from 90-160 distinct...

12. Finding Your Science Policy Path

    Type

    Blog Post

    Published

    Sept. 22, 2020, 1:15 p.m.

    ...science policy skills in graduate school via this report from the Scientific Citizenship Initiative.    ...

13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ...Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756 . https://doi.org/10.1016/j.celrep.2017.06.064...

14. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ...of reproducibility for preclinical studies, as reported by drug companies attempting to apply the findings...

15. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ..., et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated...

16. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating large DNA ...

17. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ... complexes to study neural function. Often the reporter proteins associated with these complexes are so...

18. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    Published

    March 14, 2017, 2:30 p.m.

    ...), and ability to metastasize. There have been reports of clinical trials going amiss and patient deaths...

19. Antibodies 101: Flow Cytometry

    Type

    Blog Post

    Published

    July 20, 2021, 1:15 p.m.

    ...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...

20. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...protein kinase dynamics in living cells with FRET reporters." Methods 40.3 (2006): 279-286. PubMed PMID: 16908183...