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Showing: 281 - 300 of 306 results

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Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

Published

Feb. 22, 2017, 3:30 p.m.

... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

Published

Oct. 11, 2016, 2:30 p.m.

... through Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

Published

April 9, 2019, 1:13 p.m.

...C-terminus of the fluorescent protein. I prefer to describe the different parts of the fusion from N- to C-terminus...
Five Popular Model Organisms

Type

Blog Post

Published

April 11, 2019, 12:39 p.m.

...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...
Fluorescent Proteins 101: When GFP lets you down

Type

Blog Post

Published

Aug. 23, 2018, 12:05 p.m.

...probes are based on cofactor binding proteins as described above. The smallest tags are peptides that are...
Transferable Skills Guide: Conflict Resolution

Type

Blog Post

Published

Jan. 4, 2022, 2:15 p.m.

...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...
10 Steps to a Perfect Science Talk

Type

Blog Post

Published

Aug. 23, 2016, 2:30 p.m.

...listeners to be thinking “What happens next?” Describe how you pushed to a solution or overcame obstacles...
How to Negotiate a Successful Lab Start Up Budget

Type

Blog Post

Published

Sept. 1, 2015, 2:30 p.m.

...importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools, skills...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

Published

Jan. 16, 2025, 3:42 p.m.

... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...
Viral Vectors 101: Optogenetic Tools

Type

Blog Post

Published

June 27, 2023, 1:15 p.m.

...In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

Published

May 23, 2023, 1:15 p.m.

...between the targets. Or, it might be sufficient to describe or classify the labeled structures visually; for...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

Published

Sept. 29, 2016, 2:30 p.m.

...identify possible gene function. In this post, I will describe a useful and cost-effective method that our lab...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

Published

June 24, 2020, 5:45 p.m.

...histone modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Type

Blog Post

Published

Dec. 15, 2016, 3:57 p.m.

...BAC DNA can be modified with any of the below described approaches. Bacterial strain with integrated...
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Type

Blog Post

Published

Oct. 17, 2019, 12:28 p.m.

...the opsin into the correct orientation to be transcribed (Figure 2) (Sharma and Zhu, 2014). How does FLEx...
Plasmids 101: Using Transposons in the Lab

Type

Blog Post

Published

July 6, 2021, 1:28 p.m.

... on a reverse transcriptase enzyme to reverse transcribe back into DNA before being inserted into new ...
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Type

Blog Post

Published

Dec. 17, 2020, 1:30 p.m.

...available through Addgene. In 2019, Farboud et al. described another similar co-conversion marker (repair of...
Important Considerations When Using AAVs

Type

Blog Post

Published

June 13, 2017, 2:30 p.m.

... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...
Plasmids 101: Modular Cloning Applications and Kits

Type

Blog Post

Published

May 14, 2024, 1:15 p.m.

...are designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila...
Cancer and the Immune System: Deciphering the Relationship

Type

Blog Post

Published

March 14, 2017, 2:30 p.m.

... tested and will help healthcare professional prescribe and identify potential beneficiaries. Collaborative...

Showing: 281 - 300 of 306 results

Results per page:

Deep Mutational Scanning with One Pot Saturation Mutagenesis

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Five Popular Model Organisms

Fluorescent Proteins 101: When GFP lets you down

Transferable Skills Guide: Conflict Resolution

10 Steps to a Perfect Science Talk

How to Negotiate a Successful Lab Start Up Budget

Viral Vectors 101: AAV Serotypes and Tissue Tropism

Viral Vectors 101: Optogenetic Tools

Antibodies 101: Multiplex Immunofluorescence

Technique: Probe Phage Genomes for Host Binding Proteins

CRISPR 101: Epigenetics and Editing the Epigenome

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Plasmids 101: Using Transposons in the Lab

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Important Considerations When Using AAVs

Plasmids 101: Modular Cloning Applications and Kits

Cancer and the Immune System: Deciphering the Relationship

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