We narrowed to 9 results for: scrib

... polymerases that all transcribe different genes: RNA polymerase I — transcribes genes encoding ribosomal...of the RNA polymerase to DNA. RNA polymerase transcribes DNA to mRNA, which is ultimately translated into...upstream (5’) of the sense or coding strand of the transcribed gene (Figure 1). The coding strand is the DNA...noncoding strand, as this is the strand that is transcribed by the RNA polymerase. Figure 1: Simplified promoter...can bind), and the structural genes that are transcribed together. Operon regulation can be either negative... in the opposite way. The operon is normally transcribed until a repressor/corepressor binds to the operator...translated into a protein. RNA polymerase II — transcribes messenger RNA (mRNA), which is the RNA responsible...

...validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment.... mRNA and Proteins In addition to the methods described above, you can also directly deliver mature mRNA...cells. In this case, your target cells are not transcribing DNA to mRNA, nor do they need to translate mRNA...directly combine purified Cas protein and in vitro transcribed gRNA to form a Cas-gRNA complex. You then delivery...endosomal approach. Similar to the mRNA method described above, this method is also transient, as the RNPs...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...

... made of RNA. This retroviral RNA is reverse transcribed into DNA before being integrated in the genome...encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus, the cDNA that will be integrated...lentiviral packaging methods . The three plasmids described above (envelope, packaging, and transfer) are ...

...that proteins are translated from RNA, which is transcribed from DNA. The Genetic Code The genetic code is...fact, most DNA is non-coding. These regions are transcribed into non-coding RNA (ncRNA) and play important...regulation. Regions that code for proteins are transcribed and processed into mature messenger RNA (mRNA...

... made of RNA. This retroviral RNA is reverse transcribed into DNA before being integrated in the genome...encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus, the cDNA that will be integrated...does not explain the lentiviral preference for transcribed genes. Studies comparing the lentivirus HIV and...

...cell membrane. Microbial opsins, such as those described below, can be targeted and expressed in specific...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity...

...Collection of guides that describe how popular scientific techniques work...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...