...recent resurgence of “Do-It-Yourself Science” described in Chapter 3, and of course the rapidly changing... science.”  The three chapters in this section describe the challenges of trusting science in a “fake ... of the book, called simply “Old Science,” he describes the now infamous tale of Rosalind Franklin and...changed significantly over the last decade. He describes the detection of gravitational waves--a story ...collaboration from scientists around the world. The paper describing the waves’ detection had over 1,000 authors. ...This last section of the book, Future Science, describes the author’s thoughts on science’s biggest challenges... doesn’t need to. The problems and challenges described in The State of Science have not changed in “COVID...

... friends on the Cambridge 2010 iGEM team that describes the basics of Gibson assembly as a parody of “...assembly The Gibson assembly technique was first described by Dr. Daniel Gibson and colleagues at the J. ...combined with the popular CRISPR technology and is described in a recent publication by the Lockey lab (Wang...followed by the standard Gibson assembly techniques described above, this resulted in the direct and seamless...using Gibson Assembly along with CRISPR was also described recently (Jiang et al. 2015), in which very large...challenging. Other homology based technologies We've described Sequence and Ligation Independent Cloning (SLIC...homology based assembly methods that have been described--CPEC (circular polymerase extension cloning) ...

...Detect Zika Virus The RNA-based toehold switches described in this post are engineered to bind RNA sequences...Boston University School of Medicine, this post describes a tool you can use to study basic virus biology...researcher in the Silver Lab at Harvard University, describes R-bodies, “force generating protein machines that... Connie Cepko’s Lab at Harvard Medical School describes how AAVs function and indicates important things... postdoctoral researcher in Jacob Corn’s lab, describes how you can better design your donor DNA template...from the Jackson Laboratory for Genomic Medicine described Casilio, a system that uses modular RNA binding...

...plasmids with the original publication where you described them. Search for your article as you would search...purpose of the plasmid. Once you've named and described all of the plasmids in your deposit, you should...process, you will enter plasmid data in the pages described below. You can complete these pages sequentially... Use the "Relevant mutations/deletions" box to describe any mutations within your insert using standard...lowers biosensor affinity for calcium". Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc...the page will open a new set of entry fields to describe the next insert. Repeat as needed for more inserts...Addgene plasmid #52961)".   The vector type should describe how your plasmid is intended to be used. For instance...

...minigenome is capable of being replicated and transcribed in the cell when the appropriate viral proteins...EBOV polymerase replicates the viral genome and transcribes viral genes. Four viral proteins are required...minigenome. These genes and the minigenome are transcribed (and the viral mRNAs are translated) by cellular...polymerase will use the minigenome as a template to transcribe the reporter gene (e.g. firefly luciferase). ...minigenome system Minigenome systems, such as the one described here, are extremely useful research tools. In ...polymerase also needs to be present in the cells to transcribe the viral mRNAs and minigenome. This can be accomplished...

...SSC-H, or FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram, which graphically represents... of the cell’s illumination (see Figure 2). H describes the maximum signal strength, while W results from...photocurrent vs. time. The resulting histograms is described through maximum current (H), the time the cell...depends on cell type and laser voltage. The voltage describes an electric potential that can be applied to the...comparing two markers simultaneously, we commonly describe the resulting populations as double positive (...that there are three parameters — H, W, and A — describing the intensity and duration of the detected signal...

...the transcribed gene and its 5’ UTR. Various self-cleaving hammerhead ribozyme parts are described in ...of an RSM is defined by the sequence within a prescribed region of DNA termed the “register”. Each chemical...registers within a sample using a metric that we describe in the Materials and Methods section of our paper...to read states by qPCR or Sanger sequencing as described in the Material and methods section of our paper... Multiple large serine recombinases have been described in the literature (5, 6) and may be substituted...

...of Amsterdam. The previous post in this series described a practical approach to selecting a bright fluorescent...literature are performed in different ways. We describe a number of issues with some of the experimental...fluorescence, which, in its simplest form, can be described by a mono-exponential decay (analogous to radioactive...). Consequently, the intensity decay is often described by the t½, which is the time after which half ... al, 2008; Bindels et al, 2017) and cannot be described by a single parameter. Therefore, it is necessary...

...specifically refers to the organizational schema described above, and not any particular cloning technique... functional units composed of multiple parts transcribed together. They can express one or more gene(s...assembled into larger functional units which are transcribed together. Image courtesy of Robert Hurt. ...cellular engineering goal, several of which are described below. Multigene circuits assembled through MoClo...relationship between genotype and phenotype is best described with “it’s complicated.” Applying MoClo Interested...

...or gene copy. The coding sequence for GFP is transcribed and translated along with the gene, producing... last exon of the gene to ensure it would be transcribed and translated. For an N-terminally tagged protein...each cell line. The editing strategy and design described above can be used to create similar donor plasmids...Endogenous gene tagging with fluorescent proteins as described here enables live cell imaging to study cellular...

...may not actually help. Ultimately, if you cannot describe the likely outcome with some specifics, the search...cannot either. Therefore, you need to be able to describe experimental approaches in terms of the question...the aims and clarify what type of aim you are describing. It's easy to be overly ambitious – a common ...accomplished in 5-10 years? You need to be able to describe what success looks like. If you are successful...includes the overview and the individual aims. Describe the question, the bare minimum of background needed...

...insert into RNA. In practice, the term "promoter" describes the combination of the promoter (RNA polymerase...expressed.      The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase (RNAP). In bacteria...promoter, whereas small RNAs (such as shRNA) are transcribed from the RNAP III promoters. This post features... expression mRNA Yeast adjacent, divergently transcribed promoters Inducible with galactose; repressible...identify the right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max...

...article describing a sucrose supplementation strategy to improve yield. In addition, as described in this...you time and improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification ...lab tips, and more video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources...

...database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks to identify and describe all ...authors of the paper in which the cell line was described. Using Cellosaurus Cellosaurus is searchable by...Cellosaurus received a report that HT-55, which was described as a colon cancer cell line, is actually a rectal...

...fly fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome... Mehmet Fatih Bolukbasi from Scot Wolfe's lab described a chimeric Cas9 that is improving the nuclease...also got to show off our new CRISPR page (which describes how to plan your CRISPR experiment for our new...next week, so follow us on Facebook, Twitter, or subscribe to this blog. Find more CRISPR and Genome Engineering...

... Rather than being transcribed from different promoters, the gRNAs are transcribed together and are flanked...like AAV. In addition to the mammalian option described below, plasmids for making polycistronic gRNAs...oligonucleotide assembly.  Unlike some of the plasmids described above, this vector does not contain Cas9, so you... are similar to the Csy4 polycistronic system described above, except that they use an endogenous nuclease...

...for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et ...www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found here: https...Aptamer base: a collaborative knowledge base to describe aptamers and SELEX experiments. Database, bas006...

...Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity of Cas9s isolated from different..., and NGCG). Since then, the Osamu Nureki Lab described a rationally engineered Cas9, SpCas9-NG, which...Nishimasu et al., 2018). The David Liu Lab similarly described xCas9, which displayed the ability to target multiple...post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung Lab had developed...

... to our website through the use of alt text to describe images in online spaces. Alt text is used by screen...example. Remember, our goal is to use words to describe the image in a way that helps visually impaired... your alt text, use all of this information to describe the image helpfully. You and your readers are ...write alt text for any image you’re struggling to describe in the results. And many journals accept alt text...

...both prokaryotic and eukaryotic systems must be transcribed into RNA, which occurs in three phases: initiation...Terminators are found downstream of the gene to be transcribed, and typically occur directly after any 3’ regulatory...Polymerases I, II, and III), each responsible for transcribing different types of RNA: Polymerase I is responsible...responsible for mRNA and miRNAs, and Polymerase III transcribes tRNA and other short RNAs. Although not as well...