...more closely about the wealth of high-throughput sequencing (HTS) data we generate and maintain. It became...begin! Figure 1: Discussing next-generation sequencing at the Addgene bioinformatics journal club! ...

...plasmid. Of course, other follow up methods like sequencing are more definitive, but visual identification...optimization programs to engineer 14 chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed...

... engineered genetic sequences; but it’s only the promises and associated sequence information. Addgene...

...circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that...of proteins that are highly similar in terms of sequence and thus very difficult to distinguish using standard...

...positive or negative selection). Next-generation sequencing (NGS) to identify genes of interest. ...Kampmann, M. (2023). CRISPR screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specificin...

...fluorescent signal. As for linker sequences, the amino acid sequences RSIAT and RPACKIPNDLKQKVMNH have ...

...Droplets that fluoresce contain the amplified target sequence. After all of the droplets have been read, the...reason, it can be used in the detection of rare sequences and single cell analysis. It is also being used...

...cytometric antibodies, downstream of the TUJ1 coding sequence. ES cells that stably express the dCas9-Suntag...cells were then isolated and their genomic DNA sequenced. Guide RNAs targeting several known and some unknown...

... and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional and lab...to the associated data (spreadsheets, images, sequencing data, etc.) used and/or referenced in the experiment...

...examined approximately 1,900 sgRNAs and identified sequence features and adjustments that improved sgRNA activity...Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences can also be modified to include RNA domains that...

... still able to bind to the upstream activation sequence (UAS) of its target gene, in this case a reporter...downstream of a promoter containing the target sequence of the DBD. The DBD-POI fusion (commonly referred...

...However, iChIP requires insertion of recognition sequences of an exogenous DNA-binding protein such as LexA...to suppress PCR amplification of specific DNA sequences. Thus, we've made some contributions in technology...

...is quality controlled through next generation sequencing and spectrophotometry to test purity (260/280...

... 3-6 letter representation of the gene (or DNA sequence). pBACKBONE-hGene Tip: Often researchers will ...

...science and more! All Posts from October 2016: Sequencing Options for CRISPR Genotyping Grad School Advice...

...testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged DNA and...

...for your research.     Improved nucleic acid sequence detection Feng Zhang’s lab recently developed ...

...the University of California, Santa Cruz, offers sequence data on five strains of Ebola representing the...

...and archaeal immune systems has led to extreme sequence divergence that requires a multi-pronged analysis...classification system relies on a combination of sequence similarity, phylogenetic analysis, neighborhood...that are in the same class and type don’t share sequence similarity but are grouped due to mechanisms of...

...the EBNA-1 sequence that encodes for the DNA binding protein EBNA-1 [7]. These two sequences are necessary...