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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 430 results for: sequence

Showing: 281 - 300 of 430 results

  1. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ... to ship plasmids or viral vectors containing sequences from rabies and all other lyssa genus members ...

  4. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...the PCR product should contain the edited BRAF sequence), and PCR-amplify the BRAF locus from control ... PCR product should contain the wild-type BRAF sequence). Then, mix the PCR products and use temperature... was challenging because you’re limited by the sequence of the human genome. I used the UCSC genome browser...

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...more closely about the wealth of high-throughput sequencing (HTS) data we generate and maintain. It became...begin! Figure 1: Discussing next-generation sequencing at the Addgene bioinformatics journal club! ...

  6. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...plasmid. Of course, other follow up methods like sequencing are more definitive, but visual identification...optimization programs to engineer 14 chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...itself and the degron off at the protease cleavage sequence. The tag is then shuttled to the proteasome due...the protein around, leaving a minimal additional sequence (7 kDa). This system also requires less genetic...

  8. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences are not copied...

  9. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura Duo), growing your bacteria... pCMV-Rep 78/68 SSM Library Amplification and Sequencing Protocol (DOCX, 32 KB) Screening Rinehart...

  11. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...positive or negative selection). Next-generation sequencing (NGS) to identify genes of interest. ...Kampmann, M. (2023). CRISPR screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specificin...

  13. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...Droplets that fluoresce contain the amplified target sequence. After all of the droplets have been read, the...reason, it can be used in the detection of rare sequences and single cell analysis. It is also being used...

  14. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...examined approximately 1,900 sgRNAs and identified sequence features and adjustments that improved sgRNA activity...Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences can also be modified to include RNA domains that...

  15. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that...of proteins that are highly similar in terms of sequence and thus very difficult to distinguish using standard...

  16. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...cytometric antibodies, downstream of the TUJ1 coding sequence. ES cells that stably express the dCas9-Suntag...cells were then isolated and their genomic DNA sequenced. Guide RNAs targeting several known and some unknown...

  17. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional and lab...to the associated data (spreadsheets, images, sequencing data, etc.) used and/or referenced in the experiment...

  18. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ... still able to bind to the upstream activation sequence (UAS) of its target gene, in this case a reporter...downstream of a promoter containing the target sequence of the DBD. The DBD-POI fusion (commonly referred...

  19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...However, iChIP requires insertion of recognition sequences of an exogenous DNA-binding protein such as LexA...to suppress PCR amplification of specific DNA sequences. Thus, we've made some contributions in technology...
Showing: 281 - 300 of 430 results