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We narrowed to 710 results for: tat

Showing: 281 - 300 of 710 results

  1. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...glutaraldehyde crosslinking: representative micrograph (C), particle orientation distribution and Fourier-...natively isolated complexes. To address these limitations, the Funabiki Lab developed a technique called...of LEAs may bias the distribution of particle orientations, this could be overcome with methods such as...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...recombination target sequences, denoted by triangles] facilitate tissue or cell type specificity. An on/off genetic...administered intravenously. To address these limitations, labs have developed techniques like CREATE (...broad):  enhanced coverage for inhibitory versus excitatory neurons Mice, rats Yes (LY6A) Chan et al...AAV.CAP-B10 CNS: balanced coverage of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et... its tropism is relatively more biased toward excitatory neurons than AAV-PHP.eB (Fig. 4c, Jang et al....administration of novel engineered AAV capsids facilitates enhanced transgene expression in the macaque ...

  3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...first generate your desired mutation in mouse ES cells, select for the mutation and inject the ES cells into... models. For simple knockout alleles or point mutations, the 8-10 month process of conventional gene targeting...PAM; if not, you can add a silent PAM or guide mutation. Designing an Easi-CRISPR conditional allele The...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...hearing about researchers' favorite features and limitations of existing backbones, we're excited to share...5: TurboCas protein labeling compared to immunoprecipitation. The top panel depicts the identification...gene promoter using TurboCas, streptavidin immunoprecipitation (IP), and tandem mass tag (TMT) mass spectrometry...interneurons — those marked by parvalbumin (PV), somatostatin (SST), vasoactive intestinal peptide (VIP), ...

  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...which can be switched from a default OFF to an ON state, and how you might use these in your research. Today...which can be switched from a default ON to an OFF state, as well as repressible binary systems. In recent...domain VP16. cTA binds to promoters containing putative operator sequences (CuO) (linked in groups of ...QUAS promoter is less leaky than UAS in the basal state, and co-expression of the inducer QF can increase...

  6. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...nucleotide sequences with high specificity and quantitative accuracy and even include profiling single nucleotide...Electrochemiluminescent assay for detection of extremely rare mutations based on ligase reaction and bead enrichment."...direct identification of allelic composition of mutated DNA in a mixed population of excess wild‐type DNA...probe for the detection of low-abundance point mutations." Analytical chemistry 86.1 (2013): 900-906. PubMed...

  7. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...of the population as a whole, scientists gain statistical power in their observations. However, while flow...Techniques is Best for Me?  An overview of the instrumentation A flow cytometer combines three systems to ...fusions by binding to the protein in its native state.  Antibodies-based flow cytometry can use both direct...cytometry: retrospective, fundamentals and recent instrumentation. Cytotechnology 64:109–130 . https://doi.org...

  8. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...the product either via the Sodium Acetate/isopropanol precipitation method or using a silica spin column...column available from several vendors. The precipitation method has an unfortunate side effect of messing...

  9. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ... V2.0 from it's predecessor, which had a point mutation in the puromycin resistance gene that made it ...less effective in certain cell lines. This point mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro...than creating double stand breaks and may better facilitate homology directed repair. You can find all of...

  10. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    Published
    Sept. 9, 2016, 2:43 p.m.
    ...is limited by definition, the kinome is a well-annotated collection of genes that can provide a useful ...useful for imaging or gene expression assays necessitating the screening of individual plasmids separately... gRNAs that score as hits in pooled screens, facilitating more rapid follow-up. We hope the biological...

  11. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...Also in June, the G8 Science Ministers issued a statement after a meeting in London on the global science...available to others got a nine percent bump in citations for their trouble, even when all kinds of other... as much about open access as it does about the state of peer review. 8)     That same issue of Science...

  12. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...eyestalk and transform them into a pulsating display imitating a moving caterpillar. The worm controls the snail...discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are lethal, associated with defective..."Tongue replacement in a marine fish (Lutjanus guttatus) by a parasitic isopod (Crustacea: Isopoda)." ...

  13. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...has led us to develop a searchable and sortable datatable that contains validated gRNA sequences. Validated...of the validated gRNA table You can access the datatable here. Current table columns are target gene, species... interested in. You can use the carats in the datatable headers to sort alphabetically or in ascending...

  14. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...plasmid sharing during COVID-19 Realizing this limitation, we worked closely with the legal partners and...As infection rates were increasing in the United States, and many universities and Technology Transfer ...Proteins, and plasmids from several labs at Florida State University became openly available. Find plasmids...

  15. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...but many throughout the world are affected by devastating diseases that affect far fewer people and therefore...provide hope to patients suffering from these devastating afflictions. Elia Di Schiavi - Spinal muscular...Nabavi is a Postdoctoral Fellow at the Vancouver Prostate Center and is affiliated with the University of...

  16. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...for scientific titles generated from the United States Standard Occupational Classification System  and...they are based on the US Occupational Employment Statistics (OES), but international Twitter users were included...female to male scientific authorship in the United States (0.428). 4. The #1 (re)tweeted domain by scientists...

  17. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...its gRNA, facilitating the live study of chromatin dynamics at a particular locus. A limitation of this ..., or how organization may be altered in disease states. dCas9 gets a colorful makeover Even without its...

  18. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...Office of Postdoctoral Affairs at UNC, called her presentation “Disequilibrium, Disillusion and The Postdoc...pretty much said it all.  She cited the oft quoted statistic that only 15% of current postdocs will be academic...Biology at Harvard Medical School, titled her presentation “How to Train Your PI”.   She focused her talk...

  19. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today, that changes... Plasmid sharing in Europe: Addgene’s request statistics Since 2004, Addgene has shipped more than 75,...Years of Plasmid Sharing: Interested in more statistics from Addgene's 10 years of plasmid sharing? ...

  20. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...you communicate your science, whether it’s in presentations, manuscripts, or social media. If you want ...usually ok for informal settings like lab meeting presentations. You can download larger images without the ...means the images are probably better suited for presentations and digital articles where it’s easier to give...
Showing: 281 - 300 of 710 results