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...Siliques that are mature prior to transformation are just full of seeds that you know won’t harbor your transgene...Emami, S., Yee, M. and Dinneny, J. R. (2013) ‘A robust family of Golden Gate Agrobacterium vectors for...PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method of identifying transformed Arabidopsis thaliana...

...purposes, with the Kim and Morrison method, you can just adjust the number of glass filters you use depending...columns, but the Zhao and Deng labs found they could just make their own buffers when the kits buffers ran...

...of genome math. Figure 1: Genome math illustrated. Intersect (left) and set minus (right). ...embryonic stem cells (ESCs). Your ChIP-seq finds just over 13,000 significant binding peaks for NANOG ...explore. I hope that this brief guide can help you do just that. Good luck, and always keep going. Appendix...

... is located in. Otherwise it could take us hours just to find one sample! Luckily, if a rack of tubes ...and in a way that can be easily traced. If you’re just starting a lab, or are looking for a way to organize...around or misread.  2. Track the container - It is just as important to track the location of the container...

...matter how busy I am. Believe it or not, teenagers just go to bed and you’ll never know it so don’t miss...almost every morning. Of course, this is more than just fun, as your health needs to be a priority. Pick...friends – no spouses or kids. The bottom line We don’t just “do science” – we are scientists. We have to figure...

...(Karmakar et al., 2024). This version of TnpB is just 408 amino acids long — making it one of the most... for each strand), while TnpB, like Cas12a, uses just one to cleave both strands. Similar to how Cas proteins...like Cas12a PAM sites. For Cas9, the PAM is located just downstream (3′ end) of the target sequence, while...

...countries, import and export regulations, differing customs processes, and the distances the packages needed...other things, could ensure our packages navigated customs and arrived at their destination in a timely manner...materials and information are truly accessible, not just available.   Looking forward Over the past twenty...

...an X,Y axis to generate a dot plot (Figure 1). Clustered events are called a population.                           ..., like a box, ellipse, or circle, drawn around clusters to define populations. This shape is called a ...show frequency of events, instead of relying on clustered dots. In a contour plot, each line contains the...

...prefer to review their notes, process what they’ve just learned, and ask questions later – maybe even the...your bench mentor’s training time is time set aside just for you – so do your best to show up as your most...with…now”. Ask for feedback so you can make adjustments. This could look like “What are some things I...

...ideal for studying vertebrate embryogenesis. To illustrate some of the reasons that zebrafish have been ...genes affecting diverse biological processes—not just development—are available from strain distribution...established (Felker and Mosimann, 2016). One common and robust strategy uses a transposon called Tol2 to randomly...

... or vacuum infiltration. Syringe infiltration (D'Aoust et al., 2009): Usually a syringe without a needle...MoClo system you can easily and quickly create customized vectors. For more information on MoClo for plants...his work in the Lee-Parsons Lab.     References D'Aoust, Marc-André, et al. "Transient expression of antibodies...

...the protein and RNA components, and several sgRNAs must be screened to identify effective ones. Simplified...sgRNAs targeting either transcript variant led to robust and specific upregulation of the targeted variants...neuron-optimized CRISPR/dCas9 activation system for robust and specific gene regulation." eNeuro 6.1 (2019...

...model is a standard scientific strategy. However, a robust research proposal will distinguish between competing...delivery. Design simple figures and diagrams to illustrate your vision. Practice drawing and writing everything...create a sculpture or print a 3D model to better illustrate your point.  Practice the full chalk talk with...

...The Rett Syndrome Research Trust (RSRT) has an ambitious goal of curing Rett Syndrome and Addgene is ...Syndrome Rett Syndrome was first described in 1966 by Austrian physician, Andreas Rett, who tried to raise awareness...Meyer KC, Solano S, Nguyen K , Lang E, Kaspar AA, Foust KD, Thomsen G, Fugere M, Kaspar BK (2019) Rett syndrome...

...co-infection of two AAVs. Cas9 orthologs: shorter, but just as potent and specific? The two AAV strategies described...still fitting Cas9 and its gRNAs into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9...published that deliver these Cas proteins with AAV, at just 1,108 and 986 amino acids, respectively, the size...

...array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated editing...induce the translation of any detectable protein, not just fluorescent proteins. Figure 4: Reporters ...that contained the cytidine base editor exhibited robust induction of GFP fluorescence confirming the efficacy...

...results from CRISPR edit data requires the help of robust software tools. As a consequence, a critical step...performed over multiple experiments and comparing the robustness, accuracy, and speed with existing tools. For... that a particular parameter was automatically adjusted to generate ICE results. If there are no results...

...the antitoxin is unstable, meaning that the cells must continually produce antitoxin to avoid cell death...RNA-regulated systems Toxin-antitoxin systems are not just restricted to the protein realm. Let’s take a look...-antitoxin system discovered. Unlike the systems just described, the hok/sok systems consist of three ...

...that works with Addgene in some way. For users, we just released a major redesign of how search works on...accountability on the part of each developer and creates trust amongst the team. Whether we are a highly collaborative...develop better software.  Maintenance  We’re not just about developing new features and fixing bugs: we...

...less likely to read about our work. We therefore must discover new ways to reach our colleagues and other... in many disciplines has been a long brewing frustration for me. I read a variety of psychology, business...Scott Spicer at the University of Minnesota helps illustrate this: “In terms of total view count and videos...