We narrowed to 397 results for: ARA-2

...partitioning each type of plasmid into a separate daughter cells (Figure 2) (Ebersbach et al., 2005). However...to plasmid loss (Diaz et al., 2015).  Figure 2: The partitioning problem. For low copy plasmids...Plasmids. Microbiology and Molecular Biology Reviews 62(2): 434–464. Pubmed PMID: 9618448 Diaz R, Rech J, Bouet...model, termed the “handcuff” model, iterons of two separate plasmids bind to the same RepA molecules, forming...NTPase is recruited and “walks” each plasmid to a separate daughter cell. When plasmids are compatible, different...bind to each plasmid type, and different NTPases separate the plasmid pairs into the new daughter cells.... Pubmed PMID: 22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial plasmids: classification...

...evolutionary tree at least 6-7 times and on at least 2 separate continents. Mimulus (Andrea Sweigart) Monkeyflowers...presented in a series of quick (10 minute) or very quick (2 minute) talks so we got to hear from everyone. Addgene...Organisms, Part 1 Five Popular Model Organisms, Part 2 .... Sticklebacks demonstrate unusual parallel adaptation to disparate environments so can be used study ...difficult). Cuscuta (James Westwood) Dodders are parasitic plants that steal all their food from other plants...have economic importance as harmful weeds. The parasitic fusion is such that the two plants share RNA, ...

...A-B+ cells. If you look at the dot plot in Figure 2, you might assume that all cell populations are positive... the different populations. In the case of Figure 2, one population is B- and another two are B+. Our ...are in the lower right corner.      Figure 2: This flow plot has two populations that are B+ and...actual flow cytometry run, all markers are read in parallel.  Let’s imagine we’re looking for cells in a sample...markers to most specific, the first step is to separate cells from debris and roughly group the cells ...gated population, two types of FSC are used to separate single cells from doublets, ignoring anything ...population of B cells and dendritic cells is further separated into three different populations using conjugated...

...-induced changes in root-to-shoot ratio in Arabidopsis, (2) expression patterns in plants with enhanced... go back and finish the review.  Peer-review step 2: Detailed assessment After this initial assessment... of Arabidopsis HapMap population in Amsterdam. Currently, she is a postdoc at KAUST, Saudi Arabia, in...Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. From the perspective of an author submitting ... specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories will ensure that the ...guest blogger Magdalena Julkowska from KAUST, Saudi Arabia. Magdalena Julkowska did her Ph.D. on salt-stress...

...resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after herbicide was ... CLIPf tag and a C-terminal SNAPf tag; see Figure 2), the authors developed a cost-effective expression... when associated with microtubules. Figure 2: CLIP-SNAP fusion proteins for single-molecule FRET... to drive prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable...enables heritable desired edits in tomato and Arabidopsis. Nature Plants, 10(10), 1502–1513. doi: https...

...improve solubility and stability (Figure 2).      Figure 2: Crystal structures of CRBNmidi alone...fluorescent tags in Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126...K., & Russell, W. C. (1987). Isolation and characterization of monoclonal antibodies to simian virus 5...matured considerably faster, with expression levels comparable to GFP during early embryogenesis (Figure 3)....Merritt D.M., Pe K., Cesar M., Hobert O. (2024) Comparative analysis of new mScarlet-based red fluorescent... and the LgBiT luciferase fragment (expressed separately) assemble and catalyze the luminescence reaction...

...host range plasmids. FEMS Microbiology Letters, 348(2), 87–96. https://doi.org/10.1111/1574-6968.12241  ...mobilization of broad host-range IncQ plasmids. Plasmid, 62(2), 57–70. https://doi.org/10.1016/j.plasmid.2009.05.001...negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit.22695  Schmidhauser...Understanding the characteristics of natural plasmids and how they function in nature can inform us on...trichosporium, Myxococcus xanthus, Neisseria spp., Paracoccous denitrificans, Pseudomonas spp., Rhizobium spp...Moraxella spp., Mycobacterium aurum, M. smegmatis, Paracoccus denitrificans, Pasteurella multocida, Porphyromonas...Escherichia coli, Gluconacetobacter xylinus, Paracoccous denitrificans, Pseudomonas fluorescens, P. putida...

...Brainbow mouse neural labeling system. Brainbow-1 and -2 have been further refined to create Brainbow 3.2. ...M-CFP expression; these deletions are defined using 2 loxP variants (only identical loxP sites can mediate... each fluorophore. Third, Brainbow-1 and Brainbow-2 each contained a “default” state; for example, Brainbow...processes not previously visible with Brainbow-1 and -2. The general structure of Brainbow-1.0 is retained...fluorescent proteins to find those with ideal characteristics (low aggregation, high photostability, and ...

...is unique compared to the rest of the genome and (2) the sequence is located just upstream of a Protospacer...pyogenes Cas9 variants with varying PAM sequences, 2) the use of Cas9 homologs derived from species other...Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell 163:759–771 . https://doi.... PAMs, respectively (Walton et al., 2020). Characterization of Cas9 from additional bacterial species ...additional Cas9 species PAM sequence may not be characterized Non-SpCas9's bind a variety of PAM sequences.... Furthermore, non-SpCas9's may have other characteristics that make them more useful than SpCas9. For...

...our deposit spreadsheet to complete Step 1. Steps 2 and 3 are usually very easy — our tech transfer team...columns are shown in red in the spreadsheet (Figure 2) and are described in Table 1. Some cells have drop-down...Filling out and sending the deposit spreadsheet Figure 2: Example of the deposit spreadsheet showing the first... Answer “What does this plasmid do?” in <200 characters. A descriptive purpose focuses on the practical...