We narrowed to 904 results for: CAN

...out to your peers and other mentors who can help you. You Can Talk to Your Editors I wish I had been... was “How many graduate students/post-docs?” You can find some of the more detailed questions the organizers...chosen career path,” but individual institutions can have many different names for the positions commonly...universities have simple postdoc numbers sorted out there can be gaps in knowledge of demographics and the information...environments. Slide from Paula Stephan’s Talk (All slides can be found here). Importantly, the panel offered some... panel, focused on showing researchers that they can take steps to achieve success in their careers. The...the coming months and welcome guest bloggers who can provide additional advice to trainees navigating ...

...that can happen is someone will say no, but if you don't ask, you will never get the help that can make...the most effective tactic a scientist in training can adopt to ensure opportunities in the future. Studies...relationships as part of the job application process can dramatically increase the chances of being seriously... submitted The structure and content of a resume can be crucial for a successful application. It takes...good at learning new things—this is a strength you can draw on and demonstrate in your application). Beware...During the entire process, use whatever means you can to get your resume into the hands of the hiring manager...great way to find an appropriate liaison, but you can use alumni databases, friends, and even relatives...

...probes. You can follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section... best fluorescent proteins for live cell imaging can be excited many times, thereby producing a large ...power is a property of the fluorescent protein. This can differ between fluorescent proteins in the same spectral..., the photostability of the fluorescent proteins can be directly compared. An example of a photostability...distribution. Therefore, photostability measurements can be performed with soluble fluorescent proteins or...make these measurements and hope this information can help guide your understanding of reported data. Photobleaching...loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay (analogous...

...needed, a layer can quickly be removed and disposed of without risking exposure. You can request disposable...meaning it can’t replicate and continue the infection cycle once inside the host. While the virus can still... to integrate into proto-oncogenes, which can lead to cancer. These viruses need to be handled in a BSL...useful because they’re derived from viruses that can infect people, cells, and animals. Some of these ...these viruses are completely inert, but others can cause diseases. Nervous about handling viruses? That’s ...to take, and if something does happen, ensure you can receive appropriate treatment and/or monitoring. ...as part of the viral infection process (though it can integrate at chromosome 19q13.4 qtr. (AAVS1)). For...

...examination of specific proteins within a system. This can be pretty complicated given that a single cell has... purification. In this strategy a single protein can be isolated from a complex mix using affinity chromatography... tag, on the protein. While a variety of methods can be used to successfully isolate proteins, this blog...metal ions such as Ni2+ or Co2+ (Hochuli 1988), and can elute proteins with up to 95% purity (Janknecht 1991...histidines are present in most production systems and can bind and co-elute with the protein of interest (Bornhorst...denaturants (Hochuli, 1988). In some cases, proteins can be bound under denaturing conditions to expose the...preferable over the other however, tag placement can affect protein function. B, The string of histidines...

...sequences determines the orientation of the ITR, which can either be “flip” or “flop” (Figure 2). Additionally... elements of AAV and rAAV vectors, modifications can affect gene transfer efficiency and transgene expression...2017). For example, deleting the B-B' or C-C' arms can reduce the packaging efficiency but increase transgene...important for viral genome replication, these mutations can affect transduction efficiency and subsequent transgene...sequence containing transcription factor binding sites can increase transgene expression and transduction efficiency...efficiency (Shitik et al. 2023). Transgene expression can be altered by using ITR sequences from different ...activity (Earley et al. 2020), but non-AAV2 ITRs can be used to drive gene expression at varying capacities...

...markers can be challenging! But we hope this guide can help you get started. Remember, you can always ...This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells and cancer immunotherapies...By compensating between fluorescent channels, you can correct for this spillover and more easily separate...your samples. This means that, unlike voltages, you can adjust compensation values later in your analysis...for APC (Figure 1B). For single-color samples, you can only change the compensation values for whichever....     In general, most spillover between samples can be fixed with fairly low compensation values (0-50...machines or the program you’re using, but usually you can click on the correct box and either manually change...

...stock, while you can use the remaining vial to grow and freeze additional cells that can be used as your...UV light 3-4 times a week for up to half an hour can help keep your hood contamination free, but you should...disinfectant (most people use 70% ethanol, but you can also use Incidin wipes) before and after your work...materials you’ll need to bring into the hood and can wipe them all down before you start. This will also... difficult to clean your workspace properly, but can also disrupt the laminar air flow around your work...have a light microscope close to the hood so you can view your cells regularly (both before entering the... you’re working with so that cross-contamination can be identified. Even if some mammalian cells lines...

...soul of Addgene’s mission), but legal complexities can arise when scientists are sharing their materials...publications, and commercial use often come up. An MTA can resolve those questions! MTAs protect you and your...all those benefits, we know that legal documents can get tricky. Here’s how Addgene helps: For Providers...distribute your materials under the UBMTA terms, so you can rest easy knowing your rights – and the rights of...generates the MTA and emails it straight to you, or we can send it directly to your institution’s MTA coordinator...Organizations that have opted into our eMTA system can even sign electronically or choose to auto-approve...result in quick approvals, so your plasmid requests can get to your lab faster. For Everybody: Friendly...

...performed at Addgene, this adds up to a significant time savings. You can also save yourself time if you're ...step. Read on to find protocols and tips that you can use in your own lab. How to make your own competent...competent cells because cells prepared using this kit can be transformed without heat shock. Detailed protocols...cells using the protocols provided above, then you can find the subsequent transformation protocols below... Cells prepared using the Z-competent TM buffer can be easily transformed without a heat-shock step. ...resistance gene time to be expressed.  However, you can skip this incubation step and go straight to plating...other competent cells) to ensure that your cells can be readily transformed. Compare the number of colonies...