We narrowed to 798 results for: KIN

...the clock for others to dive in. The real cost of making data accessible A more reasonable concern with ... the scientific community is thus not averse to making data available, but there remains concern about... by others. Other new data initiatives are also taking root. Nature Publishing Group recently launched...efficient searching and extraction of information. Looking a little further ahead, journals may move towards...Instead of providing a “dead end” figure or table, clicking on the published chart could reveal the underlying...reader as possible. Rather than adding to demands, making effective data sharing the norm should open up ...Canada where he studies protein-serine/threonine kinases implicated in human diseases. His spare time is...

...scientific discovery and career development. They are working with limited resources but limitless potential....by facilitating connections to mentorship and networking opportunities, publishing assistance, and more...international scientific community and are always taking steps to increase plasmid distribution and disseminate...distribution As you can see from the chart below, we are making great progress toward spreading the Addgene community...countries. As part of these efforts, we will be working with Seeding Labs to award plasmids to research...well as shine a light on some of the great minds working in developing countries. As the world continues...

...been extremely helpful for scientists that are looking for more detailed information about this new technology...topics you want to learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular...additional questions. We’re happy to see scientists making their own cells to save money and create the tools...feature in the coming year. Finally, we are always looking for guest bloggers who want to share their research...Replication By Kendall Morgan Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids...Marcy Patrick Will You Be My Mentor? Finding and Asking for Mentoring Support By Joanne Kamens Plasmids...

...of naked DNA. Other investigators who had been working to develop high efficiency gene targeting approaches...the addition of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred... CRISPR-based approaches for the generation of knockins via homologous recombination with a homologous...application - the creation of sequence-specific knockins - for which the AAV approach is most well suited...suited, since it routinely generates knockin efficiencies of 1-40% with G418-selected clones. However, there...approach requires G418 selection of infected clones, making it unsuitable for most in vivo applications.  Third... CRISPR-based approaches for the generation of knockins, it is not always ideal. Because of these limitations...

...epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment...minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds of particles...molar ratio) with 10 minutes of glutaraldehyde crosslinking: representative micrograph (C), particle orientation...could be overcome with methods such as chemical crosslinking or tilted-stage imaging. A truncated form of...compatible with the buffer additive MgCl2 and the crosslinking agent glutaraldehyde. Adding RvLEAMshort to ...j.cell.2021.03.025.    Recombinant antibodies for marking cell types in the nervous system By Ashley Waldron...filament expressed in astrocytes throughout the brain, making it a popular target for labeling these cells. Check...

... pathway. Those can include cytokines and chemokines; enzymes like kinases, phosphatases, and ubiquitin...false positive signal. Dump gating When you are working with heterogeneous cell mixes, like bulk lympho... buffer solutions. Fixing causes protein cross-linking, killing the cell but preserving the cellular state...gating uses the same color for different markers, making it easy to gate on the negative cell population...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

...in vitro kinase reactions. Often, however, researchers lack knowledge of the regulatory kinase, access ...access to active kinase for the reaction, or have difficulties with kinase specificity in vitro. The establishment...phosphorylation sites. If knowledge of a candidate regulatory kinase is known, it’s possible to generate non-phosphorylated...phosphoserine into recombinant proteins, such as the kinase MEK1 (5). MEK1 is inactive without requisite dual...create a pSer/phosphomimetic dual mutant) elevated kinase activity over 70-fold over dual phosphomimetic ...

...every day, sharing ideas for the company, but also talking about sports, movies, and just about anything. ...cite lunch as one of their favorite parts about working at Addgene. We’re a small non-profit, so we can...Chef-style competitions Every few months, we’ll have a cooking competition featuring a selected ingredient. Anyone...cook-off last week, and the featured ingredient was pumpkin. Everyone gets to vote for their favorites, and...the stiff competition or says something about my cooking.   This is a creative (and delicious) team building...work better and keeps us smiling even when we’re packing the 400th plasmid that day.   As we head into Thanksgiving...

...Does this sound familiar? Well, when it comes to working with Addgene, we certainly hope not! One of our...email. You need help right at the moment you’re looking for it, or you may just prefer to troubleshoot ...icon, search for answers, and choose a result. By clicking on the result links themselves right from within...ll navigate to articles answering your questions making it easier than ever to get the support you need...some reason you can’t find the answer that you’re looking for, or you just have a general question or concern...valuable feedback. This is Addgene after all, and making your research easier always comes first. Resources...

...have an opportunity to improve my networking with mentors who are working in USA and Europe, something that...who migrated from their country some years ago looking for their career improvement) and bright students...world in flux. The professional development and networking resources offered by programs like IMP USA-Europe...Institute of Technology, Boston University, Johns Hopkins University, the Salk Institute, and the Karolinska...and contacts with Spanish laboratories to start working on projects together. List of discussion topics...Addgene Read Other Career Blog Posts Read the Networking for Scientists Series Read our Blog Post about...