We narrowed to 511 results for: ATO

1. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...world using words like OR and AND. These logical operators are so useful they are implemented in most materials...

2. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

3. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...

4. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   Published

   Dec. 3, 2019, 11:38 a.m.

   ... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your...

5. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...

6. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   Published

   July 27, 2023, 1:15 p.m.

   ... Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological...

7. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...

8. Plasmids 101: Protein tags

   Type

   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity...

9. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   Published

   Dec. 2, 2014, 7:23 p.m.

   ...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for...

10. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ...optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection...

11. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    Published

    April 23, 2014, 1:08 p.m.

    ... the proper cell line is used; several common laboratory cell lines including 293 were immortalized with...

12. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson...

13. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...

14. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ...successfully to derive iPSCs from PBMCs. Most laboratories use Sendai virus to reprogram PBMCs efficiently...

15. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    Published

    Nov. 14, 2017, 2 p.m.

    ...and post-doctoral programs lack any formal or mandatory professional development training. Further, even...

16. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ... and is commonly used to enrich adult mouse hematopoietic stem cells. Its ligand(s) has not been identified...

17. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...

18. Supporting Reproducibility with a Connected ELN

    Type

    Blog Post

    Published

    Jan. 17, 2019, 1:38 p.m.

    ...researchers and PIs, science librarians and data curators, IT managers, the commercialization office, the...

19. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...much information as possible from depositing laboratories and associated publications, but our quality...

20. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    .... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...

21. Transferable Skills Guide: Creativity

    Type

    Blog Post

    Published

    Jan. 18, 2022, 2:15 p.m.

    ...This is why perfectionists tend to also be procrastinators.  If you fall into this category, get out of...

22. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation...

23. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type

    Blog Post

    Published

    April 13, 2021, 1:15 p.m.

    .... The pDonor plasmid pRE57-Ter with multiple terminators can serve to interrupt genes (or gene clusters...

24. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...antibiotic works. Another example is Zeocin, a DNA intercalator, which affects all kingdoms of life. Of course...

25. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...seeing our resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit...

26. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...fluorophore. The fluorescent signal can be used as an indicator to determine whether the target sequence is present...

27. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    Published

    Dec. 13, 2016, 3:30 p.m.

    ...several years ago – recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban...

28. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    Published

    Oct. 25, 2016, 2:30 p.m.

    ...paid. Institutional salary policies are one indicatator of how salaries may changes due to the FLSA. ...

29. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

30. Plasmids 101: Control Plasmids

    Type

    Blog Post

    Published

    April 9, 2015, 3:29 p.m.

    ...hypothesis: proper controls! For every experiment, an investigator needs a standard against which the results can...