We narrowed to 662 results for: Clu

...can obtain excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in ...

... 1: Schematic of Class 1 CRISPR types. Class 1 includes type I with a Cas3 effector, type III with a Cas10...

...effectors allows for a wide variety of screens, including interference, activation, and knockout. Most...

...virtual and hybrid options is more accessible, inclusive, and accommodating for everyone involved in the...

...our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors. Both inhibitory...

...multiple avenues for professional development including workshops and networking events. I took advantage...

...additional resources provided by the depositor including genbank files and protocols here. Articles Citing...

...available in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid...

...encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer (H2O2 ...

... molecules into regulation of gene expression including growth or fluorescence. There are a few examples...

...transformation method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium...

...identification of several disease-causing genes, including the RPE65 gene responsible for an autosomal recessive...

...mammalian cells. The modules used in this method include fluorescent proteins, promoters, enhancers, polyadenylation...

...various gram-negative bacterial hosts of interest, including within mixed communities and into species not ...

...PPIs that regulate numerous cellular functions, including ubiquitinoylation, kinase signaling, integrin ...

... ability to edit RNA has multiple advantages, including reversibility and use in non-dividing cells. With...

...insights into the cytoskeleton of malaria parasites including the conservation of a conoid. PLoS Biology 19:...

... multiple approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate...

...Cpf1 found and tested? Class 2 CRISPR systems, including the type II Cas9-based system, require a single-component...

...assigning a physical location to your samples, include as much detail about that location as possible....

...people in the same situation. Go to church, join a club, meet the neighbors and create a network of support...

...smFPs can result in better resolution images. Conclusion By combining advantages of FPs and epitope tags...

...Get sponsors for a training weekend for a sports club, find speakers for a meetup or recruit volunteers...

...other distant regions. They also don’t give any clues about the types of neurons involved or whether their...

...activity, for example. Useful positive controls could include: pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166...

...whether you realize it or not. These skills might include collaborating, managing, and communicating. After...

... use of other tools they use in research. This includes chat apps like Slack, Microsoft Teams, and Google...

...can also find links to cell line repositories including patient-derived iPSCs, MECP2 animal models, tissue...

...Different rates of translation by different tRNAs, including those that exhibit wobble base-pairing (a tRNA...

...dissenting opinions is to foster a culture of inclusivity. Work with and value people who think differently...

...about the whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage...

...process. When enough copies of ATP-DnaA are bound, including at the low-affinity sites, the proteins work cooperatively...

...host ranges. Popular replicons from these groups include: RK2 (IncP), RSF1010 (IncQ), and pSa (IncW)  (Schmidhauser...

...PEG-mediated transformation into plant cells. Conclusion & Future Directions TnpB represents a new generation...

... your cloning. Most sequence analysis software includes Golden Gate cloning tools that simulate the restriction–ligation...

...systemic changes, like using alt-text, captions, and inclusive design in our digital content. We have implemented...

... from many with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons...

..., Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti...

...administered, which leads to many complications, including the inability of some institutions to know exactly...

...past fifteen years. Some examples from their lab include using AAV to introduce epitope tags into the endogenous...