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Showing: 601 - 640 of 879 results

  1. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ..., the lack of caveolin 1 in breast cancer also induces changes of stromal cells in the microenvironment...transforming growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such...molecules that can be transferred to other cells, inducing epigenetic changes in targeted cells. In this ...our cancer research plasmids and resources page  Find plasmids for your research Learn about Addgene's...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...numerical designations, separated by a slash, that indicate the ITR and serotype, respectively. For example...polarities are rescued from the vector plasmid and individually packaged into the AAV capsids. As a genome conversion...scAAV) vectors promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001)....Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All Viral Vector Resources ...

  3. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...you have to know what resources exist and where to find them! Keeping good records and an organized lab ... are typically more expensive, so you’ll have to find a balance between time savings and budget savings...factors to help you choose the right cloning method Find blog posts about scientific sharing here Download... out our special collection pages to more easily find plasmids Search for a plasmid in Addgene’s repository...

  4. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...some of its dsRNA intermediates into a healthy individual to provoke an immune response and then, hopefully...degrade mRNA transcripts. RNAi activates the RNA-induced silencing complexes (RISCs), which detect dsRNA...dysfunction, it is unclear what the precise mechanism behind the production of these dsRNAs is. A major source...infection. Similarly, if endogenously synthesized dsRNA finds its way outside of the mitochondria, the same inflammatory...

  5. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...sequence information. Collect plasmid information in a binder or digital notebook and make sure you note the ...deposit your plasmids with us, it will be easy to find all of the information needed for the process. Always...primer, you need some space between your primer binding site and the first feature you want to verify because...this region, as outlined above. Avoid primers that bind within or must read through repetitive regions (...

  6. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ... are also beginning to address these issues independently; for example, Harvard’s Therapeutics Graduate... has a compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains...again gather in Boston - this time with the aim of finding solutions to some of the problems that were identified...there for STEM graduates and postdocs in academia, industry and other areas of employment? How many graduates...

  7. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...with a body of literature related to the topic. Finding reliable references and sorting out what they mean...Searching for literature My go-to search engines for finding scientific literature are Google Scholar and PubMed...When embarking on a new literature search, try to find a recent review article to give you an overview ...data. They should explain some of the rationale behind the line of experiments, what data they collected...

  8. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... developed to treat a multitude of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular...more than 300 different coactivator proteins individually contribute to cell regulation in a coherent ...and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear Receptor Signaling. Dr. McKenna...and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear Receptor Signaling.   Further ...

  9. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...protein variant lacking its endonuclease ability, to bind to genes without editing the genome (Qi et al., ...complementary to the sequence of interest. Upon binding, CRISPRa systems recruit transcription factors ...VP64 fusion protein engineered with aptamers that bind to MS2 proteins. These MS2 proteins then recruit...Resources on Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your research ...

  10. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...during undergrad. Lauren Flynn is the Addgenie behind our "Meet an Addgenie" series. Before heading off...is a more emphasis today about non-academia and industry jobs. Since there are a variety of different career...can make big changes in your career path and still find comfort and happiness there. Read all of the Meet...process, my advisor encouraged me to keep my values in mind as I applied. Thinking about this made me curious...

  11. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...poster In almost all scientific posters you will find a title, author names and affiliations, introduction...University. The idea is that the main research finding is written across the center of the poster in a...be cognizant of audience members that are color-blind; avoiding red and green is a good idea for this ... this blog post on navigating the poster session Find tips to make the most of your conference attendance...

  12. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...access to some cells and tissues, such as human induced pluripotent stem cells (hiPSCs). Nanoblades, a ...different types of CRISPR cargo including RNPs for indel formation via NHEJ, precise modification with Homology...was not detected and Nanoblade injection did not induce morbidity. Together these results demonstrate that...Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral ...

  13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ... new blog post, and live tweet from conferences. Find more tips on using social media as a scientist here...Network – Mingle with pros Where do you meet like-minded science communicators? Attend the annual flagship...started writing three years ago on a field trip in Indonesia. Since then, I have tried many things: photography...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...

  14. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...methionine (i.e. the plasmid complements the deletion). Find the Plasmids Here Plasmid features enable multiple...met deficiencies (manuscript in preparation), indicating that Met protein functions have been largely ... in communicating science to the public. You can find out more about Clare at the Biology Dept. site.  ...Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check Out Our Molecular...

  15. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... is a small piece of DNA that can replicate independently from its host’s chromosome. Although we usually...of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact...antibiotic resistance-mediated selection is indispensable for cloning work, antibiotics and plasmid-encoded...biology, especially when combined with natural inducible expression systems that allow tuning of metabolic...

  16. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...given site. While it is possible to "get lucky" and find a great gRNA with a proximal PAM right where you...solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that uses a PAM with ...flexibility could in theory promote off-target binding. The activity of SpRY was validated in double stranded... the genome! Want to get your hands on SpRY? Find a mammalian plasmid here and edit on! References...

  17. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...these tools with the Zika virus research community. Find the Zika Sensor Constructs at Addgene As we report...forward, our short-term efforts are focused on finding the resources necessary to advance the technology...Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green...Check out All the Plasmids from the Collin's Lab Find Synthetic Biology Plasmids for Your Next Experiment...

  18. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...technical challenges they can present. Many researchers find themselves plagued by troubleshooting a virus-based... during transfection, and does not necessarily indicate the production of virus. Alternatively, if the...gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes." Biophysical...Addgene.org Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ...

  19. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...become increasingly restricted to academic and industrial labs, but recently there has been a counter movement...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to ...when starting a new project at a makerspace is finding the financial resources to pursue it. Since makerspaces...area, please stop by. And if not, I suspect you can find a makerspace somewhere near you. The place to start...

  20. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ... way to bring out these interesting stories and remind the panelists of the stories or topics you know...correct date, location and parking information and remind them about the event as it approaches. Room set-up...if slides are allowed. Don't put your panelists behind a table. It adds a formality and distance that ...few questions. Who is out there? What do they do? Find out their areas of science or expertise by listing...

  21. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ... exciting story, and are ready to present your findings at a conference. But what you get out of a conference...easier through social media. Use social media to find out who’s going to the conference and who you’d ...for any relevant hashtags and handles to help you find other scientists attending. Reach out if you’d like...live-tweeting any sessions that the presenter doesn’t mind sharing on social media. If you could not attend...

  22. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...However, they can also be given retro-orbitally, behind the eye. IV injections are used for a broad range...scruffing or mechanical restraint Location: back or hind legs Gauge: 25–27 Volume: < 2–3 ml (~1 ml per site... Back scruffing or mechanical restrainer Back or hind legs; between skin and muscle 25–27 < 2–3 mL (~1...Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (2011). Administration of substances to...

  23. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ... Off Resources on Addgene.org Find Mammalian Expression Vectors Find Cre-Lox Plasmids Learn More about...with a gRNA). Be sure to keep these techniques in mind when choosing a knockout strategy! Knockout plasmids...knocked out. Use positive and negative selection to find correctly recombined cells. Recombination is a rare...

  24. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...they are transferrable across most, if not all, industries and work environments. This is why they are called...co-workers want to know that you are a well-rounded individual with more going for you than your technical expertise...develop your nascent leadership skills. You might just find that you’re more of a leader than you thought! When...into pretty much any other workplace. To get your mind working, take a look at the short list of transferrable...

  25. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...located in. Otherwise it could take us hours just to find one sample! Luckily, if a rack of tubes is dropped... barcode also identifies the contents of each individual tube.  If a plate of samples is ever dropped,...know what is in each one. Even if we lose a tube behind a freezer or under a lab bench, we will know that...container a specific location to know exactly where to find your samples. Additionally, giving each sample a...

  26. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...37C Y66W Causes the chromophore to form with an indole rather than phenol component (cyan derivatives)...one fused to each protein or domain being tested. Find FRET plasmids here. Split EGFP: An alternative to... more about optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell... Gal will share his list of 10 things to keep in mind when choosing the best fluorescent protein to use...

  27. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...the years we have accumulated more than 300,000 individual nucleotide sequences. More than half of these...Quick-stepping to a FASTA file   With our outline of individual steps to automate plasmid sequence assembly, ... of one-off scripts or programs, our goal was to find a suitable software tool that could orchestrate ... More resources on the Addgene blog:  The Power Behind NGS Plasmid Validation: SeqwellHow to Keep a Lab...

  28. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...immunology or subpopulations of cells, you’ll soon find yourself encountering flow data in the literature... will have numbers by gated populations; this indicates the percentage of total events contained in the... gated population. Heat maps are often used to indicate the relative density of cells. You can see both...presented. A horizontal line across the peak will indicate the cut-off value for a positive signal (Figure...

  29. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ... also depends on the individual library. So, on each library’s page, you can find protocols for sequencing...amplification. And as anyone who has worked with individual plasmids knows, some plasmids amplify well and...troubleshooting blog should you run into any issues.  Keep in mind that a low MOI will increase the chances of a cell...

  30. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...presenters! Labs and benches! Everything was out the window. So, much like SynBio, it was time to start our... was going to be time consuming and difficult. Finding volunteers who had space to shoot was another hurdle...people enough.  Build: If you’ve ever watched any behind the scenes footage in the last several years, you...distance. Since everyone in these shots (and me behind the camera) would remain masked, it freed us up...

  31. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...cancer cells more resistant to chemotherapy. You can find these plasmids at Addgene. Another advantage of ...model organism in scientific research. Yes, the same kind we use in breads and other baked goods! Yeast is...plasmids. Then using HOTcre, a method that uses heat induction to control the temporal expression of different...collection pages Read our molecular biology reference Find plasmids for your research ...

  32. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...talk with them about science: Representatives: To find contact information for your representative in the...you need to know is your area code. Senators: To find contact information for your senators all you need... do is select your state. State Legislatures: To find the website to contact representatives at the state...objectivity of science.  Scientific American: Scientists Find a Voice at Massive Rally for Immigrants National...

  33. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...that can be used with mutational scanning: ligand binding assays, phage display assays, ubiquitination activity...activity assays, toxin activity, small molecule binding, growth rate, and many more. See Fowler et al for...a single mutagenic primer pair to generate an individual mutant, this technique uses mutagenic primers... Mutate Your Plasmids Resources on Addgene.org Find Plasmids from the Whitehead Lab Use CRISPR Pooled...

  34. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...various research applications, they have become indispensable tools for manipulating cells and organisms. ... to cells for genome editing, and introducing inducible systems into cells, among many other uses. Lentiviral...lentiviral production protocol.  One thing to keep in mind is that titering these IDLVs may require a slightly... (2013). High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature ...

  35. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Resources on Addgene.org Find CRISPR Plasmids for Plants Read Our CRISPR Guide Pages Find Validated gRNAs for... for targeting your favorite plant gene; you can find many plasmids for a wide variety of CRISPR applications...Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool. MMBR 67:16–37 . https...

  36. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...researcher is searching for), then the probe will bind to the sample thereby labeling it. The label can...southern blotting are conducted on a sample from an individual without sickle cell anemia, two DNA fragments...sequencing could move from sequencing cores to individual labs and even out into the field. Current versions...Resources on Addgene.org Check out Our Protocol Pages Find Molecular Biology Tools Analyze Your Plasmids by...

  38. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...2015) made this step as simple as heat-shocking to induce the expression of a Cre recombinase contained within...editing efficiencies, obtaining as many as 100 independent GFP knock-ins from a single injected animal.  ...elegans has an AT-rich genome, it can be difficult to find an appropriate NGG PAM site to target. The (T)TTN...visualize specific genomic loci (CRISPR-imaging), or to find the proteins associated with a given genomic locus...

  39. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...examples of survival of the fittest. Cancer cells find a way to grow haywire, access and create more vasculature...inflammatory responses can augment tumor growth. Indeed, lasting responses to viral infections have resulted...lack of oxygen in the tumor, activates hypoxia inducing factor (HIF-1), which promotes the production ...response against tumors. Some chemotherapeutic agents induce immunogenic tumor cell deaths that help activate...

  40. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...Fluorescent Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ...system relies primarily on contrast to identify individual objects and perceive the world around us. Without...reflected. This reflection occurs due to the refractive index mismatch between the glass and the water-like buffer...
Showing: 601 - 640 of 879 results