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  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...EvolvR-ing to targeted mutagenesis." Nature biotechnology 36.9 (2018): 819. Additional resources on the...

  3. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ... not contain the trademarked Tet-On(R) sold by Clontech. For additional information, see the manual provided...

  5. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...immune response to the virus to allow it to evade detection by the host. Of note, recombinant adenovirus is...

  6. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...as FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging from ...

  7. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...over a hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around...

  8. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...added baseline component. Alternative screening techniques While CRISPR and drugs dominate the screening...

  9. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...Kaufman, et al, 2022.    This new barcoding technology would allow researchers to avoid the off-target...

  10. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... of Murine Monoclonal Antibodies by Hybridoma Technology. JoVE. https://doi.org/10.3791/54832  Lipman ...

  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...proteins yet they still share many of the same technical advantages that come with working with prokaryotes...

  17. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...achieved any end goal not available with current techniques like PGD.  Reference Ma, Hong, et al. "Correction...

  19. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...Towards Gene Therapy For Duchenne Muscular Dystrophy. InTech. doi: 10.5772/53023 Veldwijk, Marlon R., et al....

  20. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... RNA-induced silencing complexes (RISCs), which detect dsRNA in the cytoplasm and use it as a guide to...

  21. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...sensitive drug delivery systems. Elsevier Science & Technology Books. Bates A, Power CA. David vs. Goliath: ...

  26. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...dedicated scientists with expertise in sequencing technologies and bioinformatics. Through their efforts, the...

  27. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... enables rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org...

  30. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...Find protocols for standard molecular biology techniques Browse our video library Check out our molecular...

  35. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...genomic landscapes should be editable with CRISPR technology.     Fig. 1: SpCas9 has sparse PAM coverage...

  36. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods Many calcium reporters use a single fluorescent...

  38. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Gi-coupled receptor in transgenic mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth...

  39. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...AID* into genomic sites of interests via CRISPR techniques. This tool-kit includes an array of FPs as well...

  40. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...of lentiviral vector titration methods."BMC biotechnology 6.1 (2006): 1. PubMed PMID: 16836756. PubMed...
Showing: 601 - 640 of 684 results