We narrowed to 812 results for: Cre

...signaling pathway.  As a proof-of-principle, the team created an OptoNB to control the Ras/Erk signaling pathway...

...flexibility of AAV genome design in remarkably creative ways. The continued development of more and smaller...

...basic guidelines below, you’ll likely be able to create a small, usable panel.  First, fluorophores vary...

... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

...many major cancer genes. These should be used to create ever more complete “look-up-tables” of mutant function...

...leadership approach to each person, I was able to create a more productive and cohesive team environment...

...cells but with far more predictability. The beads create an artificial positive (bound) and negative (unbound...

...The catch The rapid expansion of GCE led to the creation of many new tools and potential applications. ...

...keep in mind that dam-/dcm- strains may have an increased rate of mutation (as these would also be deficient...

...harnesses the power of bacterial recombineering to create insertions, deletions, and substitutions - at the...

...Genome-Wide Control for Large-Scale Functional Screens        ...

...can make a big difference, if you can find one. Create your own peer mentoring circle. This type of program...

...toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical...

...highlights of 2018 for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline...

...inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding...

...neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However...

...limitations. New efforts have recently been made to increase cutting efficiency and diversify PAM sequences...

...proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest...

...protein-protein interactions with other peptides. Created with BioRender.com.     Many biologists can...

...more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed...

...Roth BL (2007) Evolving the lock to fit the key to create a family of G protein-coupled receptors potently...

... double-edged sword, however, and has greatly increased the burden associated with identifying plasmids...

...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

...change gave her the balance she needed. It is incredibly helpful to me to know that you can make big changes...

...Well, it used to be a lab assistant, but with increased plasmid demand it was too time consuming to complete...

...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation...

...parallel. More broadly, we drew on the combined creativity and expertise of the group and yet never hesitated...

...Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors modulating...

... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage...

...Twitter in real life. If there is not a tweep up, create one yourself. Pick a time (ex: lunch, happy hour...

...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...

...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...

...17,560 CRISPR plasmids. That’s almost a ninety-fold increase in size! From this collection, we’ve shared almost...

...hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for the membrane, where it will ...

...were having a positive experience, I was still incredibly nervous about leaving academia. A lot of academics...

...starting vector that the depositing lab modified to create their new plasmid.  The Addgene Vector Database...

...addition, there is a drop in image brightness with increasing depth caused by light scattering and absorption...

... the time it takes the FP to correctly fold and create the chromophore. This can be from a few minutes...

...improvements and adaptations that can provide an increased understanding of DNA binding and gene regulation...

...appears to return to normal. The model fit these two discrete phases with an estimate of 62.3% for the COVID...

...AAV-RPE65 treatment, famously visited Congress to help increase awareness about the potential of gene therapy!...

...makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early...

...don’t have to optically magnify quite so much. Created with biorender.com Steps 2-4 are not found in...

...deposit. Using barcoded tubes is part of Addgene’s secret to success. It helps us quickly process samples...

...’t support reproducibility as not all ELNs are created equal. Many thanks to our guest blogger, Rory ...

...with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of...

... a singleton), and is proudly "too weird to be credible." Joe is the founder and President of Binning ...

...match your expertise with that of the company. To increase your chances at successfully applying for a position...

...dLwaCas13a as a programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for...