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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 791 results for: Cre

Showing: 751 - 791 of 791 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... double-edged sword, however, and has greatly increased the burden associated with identifying plasmids...

  2. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

  6. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...Well, it used to be a lab assistant, but with increased plasmid demand it was too time consuming to complete...

  8. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage...

  10. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...

  11. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...17,560 CRISPR plasmids. That’s almost a ninety-fold increase in size! From this collection, we’ve shared almost...

  12. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

  13. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for the membrane, where it will ...

  14. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...were having a positive experience, I was still incredibly nervous about leaving academia. A lot of academics...

  15. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...

  16. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...starting vector that the depositing lab modified to create their new plasmid.  The Addgene Vector Database...

  17. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...addition, there is a drop in image brightness with increasing depth caused by light scattering and absorption...

  18. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ... the time it takes the FP to correctly fold and create the chromophore. This can be from a few minutes...

  21. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...don’t have to optically magnify quite so much. Created with biorender.com Steps 2-4 are not found in...

  22. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early...

  23. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...AAV-RPE65 treatment, famously visited Congress to help increase awareness about the potential of gene therapy!...

  25. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of...

  32. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...

  35. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...technical replicates or repeated measurements will increase the power of the results. In this case, mouse ...

  37. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ... 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert...

  39. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...to not have any effect on each other, they have discrete effects on the dependent variable, and those variables...

  41. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...detected in the same way. The standard curve that is created from the OD and concentration of the known samples...
Showing: 751 - 791 of 791 results