We narrowed to 932 results for: Gene

...the dye and the endogenous target, and thus no genetically encoded tag is required. These dyes can also ...SNAP-tag® and HaloTag®. These tags consist of a genetically encoded enzyme variant that covalently reacts... with far-red and near-infrared fluorophores. Addgene's collection contains many self-labeling protein...maintained through mechanisms like fission, fusion, biogenesis, and mitophagy, can be tracked using dyes like...Keller, P. J., Ji, N., & Lavis, L. D. (2017). A general method to fine-tune fluorophores for live-cell ...10.1016/j.bbamem.2013.09.005  More resources from Addgene Live and Let Dye: Self-Labeling Protein Tags Fluorescent...

...in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to...Fluorescent proteins (FPs) are the gold standard for genetically-encoded FRET donors (Fig. 1). Scores of FP variants...presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already...Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein of interest,...PubMed Central PMCID: PMC4380784. Resources on the Addgene Blog Get Tips for Using FRET in Your Experiments...Your Fluorescent Protein Basics Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids...

...external collaborations Resources on Addgene.org Browse Addgene's COVID-19 plasmids and resources ...efforts to shift scientific culture towards a more generous and rewarding way of making science.   How...currently based in Spain and works in the field of genetics and cancer. You can follow her on Twitter at @...mahesa2504         Resources Additional resources on Addgene's blog Check out our blog post about remote collaborations...

...reading, you can calculate the DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA...samples and are often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, .... This method is often used preceding next-generation sequencing or microarray studies and is not used...suited than another. Additional resources on the Addgene blog Read more about molecular biology protocols...different grades of plasmid DNA preps Resources on Addgene.org Visit the Molecular Biology Reference Find plasmids...

...Fatima's work and keep checking back in with the Addgene blog for future award opportunities. Xin Zhou:...when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa domains flanked...Proteins, or FLiPs.  Xin later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation...Chemical Biology! Additional Resources on the Addgene Blog Learn More about Photosactivatable Fluorescent...Fluorescent Protein for Your Experiments Resources on Addgene.org Find Fluorescent Protein Biosensors Browse Plasmids...

...specifically how dry ice can affect virus from Addgene. Bottom line: there’s good news and there’s bad... results. Order Ready to Use Viral Preps from Addgene Measuring the pH of our samples: What we did To... recommend that once you receive your AAV from Addgene, you store the AAV at -80°C for 96 hours prior ... PMCID: PMC4420252 Additional Resources on the Addgene Blog Learn How You Can Use the Viral Service Tips...Your Lentivirus Preps Additional Resources on Addgene.org Check out the Viral Service Find Virus Protocols...

...your foot in the door Resources on Addgene.org Check out how Addgene uses videos for science communication...yourself how every piece of feedback you receive generalizes.  If someone suggests a change to a slide, think... science communication, including the teams at Addgene, ComSciCon, and many more great organizations. ...entertainment industry.       Additional resources on the Addgene Blog Learn about careers in science communication...

... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...expression and protein interactions, including epigenetic modifications. But it's not an approach for the... this investment can well be worth it once you generate your data. However, it may be a good idea to add...  References and Resources More resources on Addgene’s blog Antibodies 101: Immunoprecipitation  Antibodies...

... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics:...antibody for a…less sensitive moment.   Fig 1: Blugene fading into darkness because they accidentally ...accidentally left their antibodies out. It's okay, Blugene!    Buffers  You know what temperature to store your.../help/spectral-overlap/   More resources on Addgene’s blog Antibodies 101: Monoclonal Antibodies Antibodies...

... Labeling in Flow Cytometry Resource on addgene.org Addgene's Antibody Guide rAb Affinity Purification...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...Harvard Medical School.   More resources on the Addgene blog Antibodies 101: Flow Cytometry Conventional...Purification Protocol Video rAbs in the Addgene repository...

.... Different setting, different population, new genetic variation, or different handling can all be important... tested with a new experiment; and hypothesis generation, in which data are investigated to look for unexpected...trends or differences between groups. Hypothesis generation is critical for advancement, but we are too often...using hypothesis testing statistical tools that generate a p-value.  As an example, if you hypothesize ...clear distinction between hypothesis testing and generation, can be sure that results will be reported regardless...

... can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced in animals...instead of via recombinant DNA, they can’t be genetically tagged and instead are conjugated to reporters...monoclonal antibodies, but they are laborious to generate and costly to share. What is a RANbody? A RANbody...PubMed PMID: 29440485. Additional Resources on the Addgene Blog Learn more about using the secondary nanobody...Check out this post. Additional Resources on Addgene.org More nanobody expression plasmids can be found...

...Additional Resources on the Addgene Blog Volunteer to write for the Addgene blog Listen to Our Podcast...Communication Posts Resources on Addgene.org Explore careers at Addgene Other Resources The National ...NEI), where she wrote about NEI research for a general audience. She then got a full time position with...

...attendees to read and comment online! Want the general public (say, policymakers or community organizations...the manuscript prior to submission. Preprints at Addgene Preprints are all about sharing scientific knowledge...more openly — which is also our mission here at Addgene! Along with other open-science organizations, we...significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in the final journal...10.1371/journal.pbio.3000116 More resources on the Addgene blog Summer SciComm Series: Modes of Communication...

...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting...currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments...because it helps me see problem solving as a generalizable process.  Step One: Identify the problem ...choose.    Resources Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

...  Resources on addgene.org Addgene Antibody Guide Ready-to-use antibodies from Addgene More Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to ..., Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant...

...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and...Solution: In Zhou et al, the Lin lab present a generic design for creating photoswitchable kinases and...possible to create other photoswitchable kinases? The general design of psMEK1 also worked for creating three...PMCID: PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent...Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable...

... develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene Blog Which...the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural affinity and...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...

...Science Careers Resources on Addgene.org Find Career Opportunities at Addgene  ...leading up to this date, as discussed in a previous Addgene blogpost and more recently in a paper published...There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary and Beth Israel...Research @FORsymp.   Additional Resources on the Addgene Blog Read Gary's Previous Article: Changing Labor...

...Resources on Addgene.org: Sign up to receive Addgene’s Science Career Guide Check out Addgene’s job openings...interviewer,” you can find lists that have lots of general questions about job responsibilities, performance...but then relax.  Additional resources on the Addgene blog: Tips for writing a good cover letter 6 Steps...

...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...started:  Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one...today, Soapbox Science (hosted by Nature), and Addgene are open to guest contributions. If you decide ...BananarootsBlog.   Additional Resources on the Addgene Blog Check out Other Science Communication Posts...

...especially when trying to explore the links between genetics and anatomy, which are not yet fully understood...many different mutants because the Caenorhabditis Genetics Center (CGC) at the University of Minnesota has...Makerspaces like ours can also make accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at...folks at the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline...resources to pursue it. Since makerspaces are generally funded on a membership model - everyone contributes...

...partner with Addgene. What are the most important issues for our partners and how can Addgene work with ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting...Richard D. Lewis Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

...and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant...Overall, spectral flow cytometry has the ability to generate incredible amounts of data from a single experiment...early life.    Resources More resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies...

...be able to tell you the laser configuration. In general, if two fluorescent markers are read by the same...to become a perpendicular vertical line.     In general, most spillover between samples can be fixed with...anyone just starting to use flow cytometry, it’s generally recommended to start compensating with single-... compensation panels below each plot.  The general rules of compensation apply to your experimental...Harvard Medical School.   More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

... overexpress a recombinant protein? It is not generally in a cell’s best interest to overexpress a protein...for affinity and purification An affinity tag, generally a relatively small sequence of amino acids, is...interest would eventually get ridiculous (you generally don’t want more tag than protein), 2-3 tags is...using plasmids found in this paper (available at Addgene).   Table 2: Protease recognition sites commonly...purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts of interest: Which Fluorescent...

...Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection... it also has several drawbacks. Direct ELISAs generally have higher levels of background staining. Antigen-containing...luck and happy binding! More resources on the Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting...

... Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...that person before ever asking them for work. Addgene’s very own Joanne Kamens, a strong advocate for ...Scientists on docjobs.com Additional Resources on the Addgene Blog Check Out Career Topic Page Tips on Writing...

...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about...tests must be met. In the Department of Labor’s general guide to the effect on higher education, you can...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...

... resources at Addgene Addgene's Antibody Guide What is an Antibody? animation Addgene's Antibody 101 eBook...sure where to start or what your options are? Addgene's latest animation is here to help! "Antibodies...

... Here at Addgene we’re getting into the holiday spirit and we hope you are, too! Bluegene is eager to...and tweeting it with the tags #DeckTheLab and @Addgene. Christmas, Hanukkah, ugly sweaters, Flannel Fridays...lucky winner? Happy holidays from all of us at Addgene! We look forward to seeing how you #DeckTheLab!...

... are essential for processes such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction...transduction. Biotinylation is the general process by which biotin is added to a protein or macromolecule... plasmids that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin can be added to...PubMed PMID: 8245422. Additional resources on the Addgene blog In vivo biotinylation of bacterial fusion...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Check out additional microbiology resources Stem cell models for...additional plant biology blog posts Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...particularly genetics research, for decades. Its short life cycle, reasonably close genetic homology to...demonstrate the new system in a video filmed at Addgene:     If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...

...We’re big fans of all our depositors here at Addgene — your contributions make the repository what it... designing a novel topology, then iteratively generated 3D models and amino acid sequences that got closer...Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution Ras...crosslinked peptides proposed as a scaffold for a new generation of drugs, all developed with their protein design...

...signs of holiday spirit still linger on around the Addgene office as we peruse the #DeckTheLab submissions...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan... @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com/bDRqAKrWHz — Dyer Lab...winners will receive a holiday care package from Addgene. We hope all of our entrants had fun decorating...

...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn...approaches for lab sustainability Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

... event – all while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help...all the words of support from scientists using Addgene in their labs. And it has been exciting to learn... I heard an impressive talk about the use of optogenetics tools to inhibit epileptic seizures in a mouse...McGovern Institute for Brain Research) – and an Addgene depositing scientist – stop by our booth to say...

...KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is...custom KTR design, plasmids are available from Addgene! References Kudo, T., Jeknić, S., Macklin, D.N...KTR plasmids here! Additional Resources on the Addgene Blog Learn about photoswitchable kinases Learn...protein phosphorylation Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors...

...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.... During FACS, the sample stream oscillates to generate droplets that are charged as they pass through.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...fluorescent reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will ...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...

...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Find...Find lab tips for your research Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration...sequencing capabilities to complement our next-generation sequencing, setting new standards for plasmid...online and in-person, you helped us understand how Addgene can better serve the global scientific community...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...

...protein. The pCri system, recently deposited to Addgene by F. Xavier Gomis-Rüth, aims to take the guesswork... either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The ...visualization of the data from RSCB PDB Resources on the Addgene Blog Plasmids 101: E. coli strains for protein...expression Plasmids 101: Protein tags Resources on Addgene.org Find Additional Cloning Kits ...

...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...reviewing this blog post. Additional Resources on the Addgene Blog Learn about Homology Directed Repair 3 Tips...Efficiency How to Design Your gRNA Resources on Addgene.org Check out CRISPR Topic Page Find CRISPR Nickase...

...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...body, cellular biology, medical specialities, and general items. Individual images as well as collections...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...

...scientists here Resources on Addgene.org Learn more about careers at Addgene ...posts in this series! Additional Resources on the Addgene Blog Learn more about alternative science careers...creative science education tools? Check out this Addgene podcast with educator and science rap mastermind...

...communications efforts.  Scicomm definitions (as used by Addgene)    Science communication, or science writing, ...of knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers...AP biology classes Creating a podcast about genetics using dog breeds as an example adults 45-55 yrs... you next time!  Additional resources on the Addgene blog 10 Ways to Share Your Science Science Communication...

...Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...not permeate through your tissue samples as homogeneously as a single antibody. Using a secondary antibody...10.1177/38.7.2355173  Additional resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence...