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TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry...
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New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....(2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded... -
How to Be an Excellent Trainee
TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m....days or weeks), look over your notes and protocol(s). Is there enough information there for you to be ... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....992. https://doi.org/10.1038/nbt.3625 Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m.... Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse ... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m.... standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very similar buffers... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m.... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m.... Find PE6 plasmids here! Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m.... --very-sensitive-local -L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X. Since the... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m..... 2007 Sep; 32(9): 407-14. Zimmermann T, Terjung S. FRET Basics and Applications: An EAMNET Teaching ... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....interest will be expressed. The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m.... not to do this. For a family with working parent(s), the sooner your kids can make a decent meal, fold...