We narrowed to 354 results for: {{{s}}}

1. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ...traces in the region around the guide binding site(s). The sequence base calls from the .ab1 file are also...

2. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   .... 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed...

3. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...Nature Methods 9, 787–790 (2012). 3. Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli...

4. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with...

5. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry...

6. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ...Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies: site-specific labeling...

7. Transferable Skills Guide: Career Planning Resources

   Type

   Blog Post

   Published

   Nov. 14, 2017, 2 p.m.

   ...20140417174121-34888774-linkedin-etiquette-guide-20-do-s-don-ts Resources for getting into grad school: https...

8. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ...the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell...

9. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

10. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ...

11. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ... standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very similar buffers...

12. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ... Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein...

13. How to Be an Excellent Trainee

    Type

    Blog Post

    Published

    Sept. 26, 2023, 1:15 p.m.

    ...days or weeks), look over your notes and protocol(s). Is there enough information there for you to be ...

14. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    ...10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...

15. Getting to Know Expansion Microscopy

    Type

    Blog Post

    Published

    June 20, 2023, 1:15 p.m.

    ...992. https://doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical...

16. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...

17. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome...

18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID...

19. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ... Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse ...

20. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ... --very-sensitive-local -L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh...