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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 705 results for: ANG

Showing: 301 - 320 of 705 results

  1. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ... tremendously complicated entities with a large range of characteristics (color, brightness, photostability...FPbase.org FPbase is a project written in Python & Django, using a popular database management system (PostgreSQL...users to see when a given page has unmoderated changes and to view previous versions.  Currently, all ...

  2. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas ...In addition to publishing in Science, Professor Zhang deposited the associated plasmids with Addgene. ...School of Management. His research focuses on a range of topics including biotech and research tools, ...

  3. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...can feel lost. Use this post to explore the wide range of careers available to scientists and open your...carry out in-depth research is applicable to a wide range of industries and is a highly desirable quality ...SAS, HTML, Matlab etc) are highly desirable in a range of roles, including: Science Reporter Technical...

  4. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...This guest post was contributed by Ally Huang is a 4th year PhD student at MIT. While I had always enjoyed...make learning biology more fun – bit by bit. Ally Huang is a 4th year PhD student at MIT in Biological Engineering... can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular synthetic...

  5. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1), some of the exciting...recombination. Find PhoCl plasmids at Addgene. Zhang W, et al. Nature Methods. 2017. PubMed PMID: 28288123... also provided a detailed protocol at Protocol Exchange. Plasmid maps can be found at Addgene and in Figure...flame depositor David Liu’s lab increase both the range and specificity of base editing. Kim et al. used...

  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...the VSV-G envelope provides the widest tropism or range of cells a virus can infect. Note: While both lentiviruses...and γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (... vice versa. Envelope plasmids may be used interchangeably. Are γ-retroviruses replication incompetent...

  7. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...microbial SSAPs. Image from Wang, et al, 2022.  Find dCas9-SSAP plasmids here! Wang et al. Nat Cell Biol....target. Across all loci tested, error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error...

  8. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...heavy (blue) chains as driven by two CMV promoters (orange). Figure from Andrews, et. al., Elife  A limitation...step would be to validate your new construct by Sanger sequence analysis. But for this research, it was...express multiple subclasses of IgG antibody. Broad range secondary antibodies are commonly used in research...

  9. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...these reporters, editing typically results in a change in the fluorescent readout. In this blog we will...for easy screening of different gRNAs by simply changing the gRNA used when transfecting your cells.  A...GEmCherry2. The Denham lab made several other small changes creating 2 other versions but these did not surpass...

  11. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...bacteria out of their optimally suited temperature ranges and make them grow better at suboptimal ones, which... of Earth’s harshest environments: corrosive pH ranges, crushing pressures, near-melting temperatures,...discussions. Not only are such endeavors a welcome changeover from conventional academic settings, these are...

  12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...enzymes function across a much broader temperature range than BioID, which only functions optimally at 37... Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way we detect viral outbreaks Article contributed...equipment. What’s more, detection is based on color changes to the assay paper, so no special equipment or ...

  13. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome...tracrRNA? Yes No dsDNA cleavage Blunt end 5' overhang PAM sequence NGG TTTV PAM site preserved? ...Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease...

  14. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...articulating why the results you've measured should change established theory. So, what can you do to ensure...you receive generalizes.  If someone suggests a change to a slide, think about whether the same principle...explain why the work is important and how it should change how the members of your audience think or act.   ...

  15. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...overlaps with the XbaI cut site (depicted as an orange line) because the restriction site is followed ...mutated plasmids which can later be verified by Sanger sequencing. How can I tell if my enzyme will cut...will be blocked by methylation and how that may change your expected digest results. If Dam methylation...

  16. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...upon themselves to teach me Python, a programming language favored by biologists. Almost as soon as I began...like a wet lab experiment I use the text editor TextWrangler to record the following, which are later printed...that further helps you keep track of your exact changes. Alternatively, you can simply make a note in your...

  17. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...paper, Yinong Yang’s group did just that as part of their CRISPR work in rice plants. Yang was intrigued...endogenous tRNA-processing system. Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11)...

  18. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ....  Here are some tips to get out of the rut of hanging out with only your lab mates (let’s face it, you... can also see and offer congratulations on job changes and promotions.  Follow academic scientists on ...just a meeting, but having lunch twice turns a stranger into a friendly colleague. Recommended Reading...

  19. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and grown ...much learning, the focus of the application was changed, but Sample6 still leverages the core engineering...better than current technology and get customers to change behavior. When you are ready to commit to the company...

  20. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...nutrient and ionic balance of the cells,” says Dr Sangeetha Iyer, ACD’s Scientific Advisor. It’s possible ...Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when I was a newly ... Jennifer Tsang...
Showing: 301 - 320 of 705 results